1997
DOI: 10.1073/pnas.94.22.11917
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Abstract: Understanding the mechanism for sucroseinduced protein stabilization is important in many diverse fields, ranging from biochemistry and environmental physiology to pharmaceutical science. The current study measures the preferential exclusion of 1 M sucrose from a protein drug, recombinant interleukin 1 receptor antagonist (rhIL-1ra). It is proposed that the degree of preferential exclusion and increase in chemical potential are directly proportional to the protein surface area and that, hence, the system will … Show more

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Cited by 197 publications
(215 citation statements)
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References 33 publications
(44 reference statements)
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“…Based on thermodynamic arguments, it has been argued that certain viscogens, such as in particular sucrose and glycerol, may be entirely excluded from the protein hydration shell. 63,64 This conclusion has found support from experimental techniques such as high precision densimetry, 46 neutron scattering experiments, 47 or the vibrational lifetime of a surface exposed protein labels. 48 While the question whether or not specific interactions of the viscogen molecule with the protein play a role might depend on the particular process studied, in our case they clearly do.…”
Section: Discussionmentioning
confidence: 55%
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“…Based on thermodynamic arguments, it has been argued that certain viscogens, such as in particular sucrose and glycerol, may be entirely excluded from the protein hydration shell. 63,64 This conclusion has found support from experimental techniques such as high precision densimetry, 46 neutron scattering experiments, 47 or the vibrational lifetime of a surface exposed protein labels. 48 While the question whether or not specific interactions of the viscogen molecule with the protein play a role might depend on the particular process studied, in our case they clearly do.…”
Section: Discussionmentioning
confidence: 55%
“…For example, it has been argued that both glycerol and sucrose are highly preferably excluded from the protein surface, [46][47][48] that gylcerol preferentially hydrates proteins, 60 and that these viscogens affects ligand dynamics in myoglobin only via the solvent viscosity, but not in a viscogen-specific way. 3 On the other hand, these viscogens also act as osmolytes and/or crowders, which often work through direct interactions with the protein, e.g., by attenuating the H-bond strength between polar protein residues and water, 61 or by interaction to the protein backbone with different strength.…”
Section: Merging Experimental and MD Resultsmentioning
confidence: 99%
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“…trehalose, sorbitol and mannitol) via their influence on the organization of and properties of solvent water. Other variables that can affect solubility of aqueous or membrane proteins include salt type/concentration, pH, temperature, reducing agents, type/concentration of detergent and other surfactants Arakawa & Timasheff, 1985;Bhat & Timasheff, 1992;Kendrick et al, 1997;Chi et al, 2003;Kaushik & Bhat, 2003;Lins et al, 2004;Timasheff, 1998;Valente et al, 2005). As noted previously, measured B values provide a qualitative assessment of protein-protein interactions, with more positive or more negative B values indicative of increased repulsion or increased attraction, respectively.…”
Section: Figurementioning
confidence: 99%
“…those conditions with B values within the crystallization slot). The approach of using an incomplete factorial (Kendrick et al, 1997) of crystallization conditions and outcomes combined with ANN analysis has previously been reported by DeLucas and coworkers (DeLucas et al, 2003(DeLucas et al, , 2005. The ability to improve or maximize the solubility of purified proteins is beneficial to many different protein research disciplines.…”
Section: Figurementioning
confidence: 99%