Prediction of breast cancer using volatile biomarkers in the breath

Phillips, Michael; Cataneo, Renee N.; Ditkoff, Beth Ann; Fisher, Peter; Greenberg, Joel; Gunawardena, Ratnasiri; Kwon, C. Stephan; Tietje, Olaf; Wong, Cynthia M.

doi:10.1007/s10549-006-9176-1

Cited by 200 publications

(158 citation statements)

References 10 publications

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“…Our study showed that compared with lung squamous cell carcinoma, these VOC levels were significantly higher in lung adenocarcinoma (17). Breath analysis was used to detect not only lung cancer but also breast cancer (18,19) and tumors of the head and neck (20). Regretfully, there were no related reports to date available on the diagnosis of HCC.…”

Section: Introductionmentioning

confidence: 76%

The Screening of Volatile Markers for Hepatocellular Carcinoma

Qin

Liu

Song

et al. 2010

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Breath analysis became promising for noninvasive diagnoses of cancer with sophisticated spectrometry technology introduced. This study aimed to screen volatile markers for hepatocellular carcinoma (HCC).Methods: Breath samples were collected from 30 HCC patients who were comorbid with type B hepatitis and cirrhosis and from 27 hepatocirrhosis patients and 36 healthy persons, both taken as controls. The volatile organic compounds in the samples were analyzed with gas chromatography/mass spectrometry and the markers were selected by comparing their levels between groups. Each of the markers was evaluated by receiver operating characteristic (ROC) curves and a discriminant function using the markers was established. The relationships of α-fetoprotein (AFP) levels and clinical stages with the concentrations of the markers were also investigated.Results: 3-Hydroxy-2-butanone, styrene, and decane were screened as potential markers, among which 3-hydroxy-2-butanone was found to have the best diagnostic value. The diagnostic function using these markers had a sensitivity of 86.7% and a specificity of 91.7% between HCC patients and normal controls and a sensitivity of 83.3% and a specificity of 91.7% by cross-validation. No statistically significance (P > 0.05) was found for the concentration differences of these markers between HCC patients with AFP >400 or <400 μg/L or between stage I-II and stage III-IV patients.Conclusion: These volatile organic compounds could be useful as breath markers of HCC patients, independent of AFP levels or clinical stages.Impact: Breath analysis could be useful for early diagnosis of HCC, especially for AFP-negative HCC.

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Section: Introductionmentioning

confidence: 76%

The Screening of Volatile Markers for Hepatocellular Carcinoma

Qin

Liu

Song

et al. 2010

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Factors affecting the great variability in the composition of human breath include physical condition, general health of the subject, food intake, environmental influences and overall lifestyle (Amann et al, 2004;Libardoni et al, 2006). (Wehinger et al, 2007), acetaldehyde , heptanal (Phillips et al, 1999a,b;Deng et al, 2004a,b;Phillips et al, 2006); hexanal (Phillips et al, 1999a,b;Deng et al, 2004a,b) Ketones 1-Phenylethanone (Phillips et al, 1999a,b) Hydrocarbons Benzene (Yu et al, 2005;Phillips et al, 1999a,b;Poli et al, 2005); toluene (Poli et al, 2005); styrene (Yu et al, 2005;Phillips et al, 1999a,b;Poli et al, 2005); xylenes isomers (Phillips et al, 1999a,b;Poli et al, 2005); trimethylbenzenes isomers (Phillips et al, 1999a,b;Poli et al, 2005); propylbenzene (Yu et al, 2005;Phillips et al, 1999a,b); ethylbenzene (Poli et al, 2005) 2-Methylheptane (Phillips et al, 1999a,b); 3-methylnonane (Phillips et al, 1999a,b); 3-methyloctane (Phillips et al, 1999a,b); 2-methylpentane (Poli et al, 2005); 2,2,4,6,6-pentamethylheptane (Phillips et al, 1999a,b;Poli et al, 2005); 2,4-dimethylheptane (Phillips et al, 1999a,b); pentane (Poli et al, 2005); heptane (Poli et al, 2005); octane (Poli et al, 2005); decane (Yu et al, 2005;Phillips et al, 1999a,b;Poli et al, 2005); undecane (Yu et al, 2005;Phillips et al, 1999a,b); methylcyclopentane (Phillips et al, 1999a,b); isoprene (Phillips et al, 1999a,b;…”

Section: Endogenous Volatile Markers and Their Clinical Applicationsmentioning

confidence: 99%

“…It is a major cause of death among adult and its incidence is increasing globally. A number of studies have been carried out in order to characterize substances in breath samples from patients with and without lung cancer (O'Neill et al, 1988;Petri et al, 1988;Phillips et al, 1999a,b;Deng et al, 2004a;Poli et al, 2005;Yu et al, 2005;Phillips et al, 2006). In 1988, two groups of researchers separately reported some VOCs as potential lung cancer marking substances (O'Neill et al, 1988;Petri et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Human exhaled air analytics: biomarkers of diseases

Buszewski

Kęsy

Ligor

et al. 2007

Biomedical Chromatography

674

531

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Over the last few years, breath analysis for the routine monitoring of metabolic disorders has attracted a considerable amount of scientific interest, especially since breath sampling is a non-invasive technique, totally painless and agreeable to patients. The investigation of human breath samples with various analytical methods has shown a correlation between the concentration patterns of volatile organic compounds (VOCs) and the occurrence of certain diseases. It has been demonstrated that modern analytical instruments allow the determination of many compounds found in human breath both in normal and anomalous concentrations. The composition of exhaled breath in patients with, for example, lung cancer, inflammatory lung disease, hepatic or renal dysfunction and diabetes contains valuable information. Furthermore, the detection and quantification of oxidative stress, and its monitoring during surgery based on composition of exhaled breath, have made considerable progress. This paper gives an overview of the analytical techniques used for sample collection, preconcentration and analysis of human breath composition. The diagnostic potential of different disease-marking substances in human breath for a selection of diseases and the clinical applications of breath analysis are discussed.

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“…Thousands of VOMs in trace amounts are present in human breath [12][13][14][15][16] and different studies have shown that the VOMs profile in patients with lung cancer can be discriminated from those of healthy subjects [12,17,18]. Urine has been the preferred biological fluid since compounds are concentrated by the kidney before excretion [19].…”

Section: Introductionmentioning

confidence: 99%

Solid phase microextraction, mass spectrometry and metabolomic approaches for detection of potential urinary cancer biomarkers—A powerful strategy for breast cancer diagnosis

Silva

Passos

Câmara

2012

Talanta

144

119

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a b s t r a c tA sensitive assay to identify volatile organic metabolites (VOMs) as biomarkers that can accurately diagnose the onset of breast cancer using non-invasively collected clinical specimens is ideal for early detection. Therefore the aim of this study was to establish the urinary metabolomic profile of breast cancer patients and healthy individuals (control group) and to explore the VOMs as potential biomarkers in breast cancer diagnosis at early stage. Solid-phase microextraction (SPME) using CAR/PDMS sorbent combined with gas chromatography-mass spectrometry was applied to obtain metabolomic information patterns of 26 breast cancer patients and 21 healthy individuals (controls). A total of seventy-nine VOMs, belonging to distinct chemical classes, were detected and identified in control and breast cancer groups. Ketones and sulfur compounds were the chemical classes with highest contribution for both groups. Results showed that excretion values of 6 VOMs among the total of 79 detected were found to be statistically different (p < 0.05). A significant increase in the peak area of (−)-4-carene, 3-heptanone, 1,2,4-trimethylbenzene, 2-methoxythiophene and phenol, in VOMs of cancer patients relatively to controls was observed. Statiscally significant lower abundances of dimethyl disulfide were found in cancer patients. Bioanalytical data were submitted to multivariate statistics [principal component analysis (PCA)], in order to visualize clusters of cases and to detect the VOMs that are able to differentiate cancer patients from healthy individuals. Very good discrimination within breast cancer and control groups was achieved. Nevertheless, a deep study using a larger number of patients must be carried out to confirm the results.

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Prediction of breast cancer using volatile biomarkers in the breath

Cited by 200 publications

References 10 publications

The Screening of Volatile Markers for Hepatocellular Carcinoma

The Screening of Volatile Markers for Hepatocellular Carcinoma

Human exhaled air analytics: biomarkers of diseases

Solid phase microextraction, mass spectrometry and metabolomic approaches for detection of potential urinary cancer biomarkers—A powerful strategy for breast cancer diagnosis

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