Predicting and Testing Physical Locations of Genetically Mapped Loci on Tomato Pachytene Chromosome<i>1</i>

Chang, Song Bin; Anderson, Lorinda K.; Sherman, Jamie; Royer, Suzanne M.; Stack, Stephên M.

doi:10.1534/genetics.107.074138

Cited by 36 publications

(41 citation statements)

References 40 publications

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“…For tomato, SC spreads were prepared using the technique described by Peterson et al (1999) as modified by Chang et al (2007). For EM, the same procedures were used except the slides had been coated with 0.6% polystrene-acrylo-nitrile (Sigma) in dichloroethane.…”

Section: Sc Spreadsmentioning

confidence: 99%

“…In addition, sucrose-spread SCs from tomato were prepared using the procedure first described for lily (Anderson et al 1994). The initial procedure was the same as described by Chang et al (2007) except that primary microsporocytes from five tomato buds were used. After digestion, the protoplasts were treated with an equal volume of medium that contained 1% Triton X-100, and the mixture was layered onto a sucrose-step gradient composed of equal (0.4ml) volumes of 1.5 and 2.4M sucrose solutions (each containing 1% polyvinyl pyrrolidone and 0.1% Triton X-100, pH5.1) in 7 × 50-mm centrifuge tubes.…”

Section: Sc Spreadsmentioning

confidence: 99%

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Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

et al. 2008

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Early recombination nodules (ENs) are multiprotein complexes that are thought to be involved in synapsis and recombination, but little is known about their components or how they may be involved in these events. In this study, we describe the cytological behavior of a possible EN component, MRE11, a protein that is important for the repair of the numerous, programmed deoxyribonucleic acid double-strand breaks (DSBs) that occur early in the meiotic prophase. By immunofluorescence, many MRE11 foci were associated with chromosomal axes during early prophase I in both wild-type Arabidopsis and tomato primary microsporocytes. Similar patterns of MRE11 foci were observed in two Arabidopsis mutants (Atspo11-1 and Atprd1) that are defective in DSB formation and synapsis. In tomato chromosomes, MRE11 foci were more common in distal euchromatin than in proximal heterochromatin, consistent with known EN patterns. However, electron microscopic immunogold localization demonstrated that only about 10% of ENs were labeled, and most MRE11 label was associated with synaptonemal complex components. Thus, in plants, MRE11 foci are not dependent on DSB formation, and most MRE11 foci do not correspond to ENs. More generally, our results show that the simple presence of large numbers of fluorescent foci associated with synapsing chromosomes is insufficient evidence to equate these foci with ENs.

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Section: Sc Spreadsmentioning

confidence: 99%

Section: Sc Spreadsmentioning

confidence: 99%

Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

et al. 2008

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“…Meiotic pachytene chromosomes are superior to somatic metaphase chromosomes for FISH mapping 1 resolution (Cheng et al 2002). Integration of genetic linkage maps with pachytene chromosome-based cytogenetic maps has been reported in several plant species (Cheng et al 2001;Islam-Faridi et al 2002;Howell et al 2005;Kim et al 2005;Kulikova et al 2001;Walling et al 2006;Wang et al 2006;Amarillo and Bass 2007;Chang et al 2007;Koo et al 2008;Szinay et al 2008;Tang et al 2008, accompanying article, this issue). We report FISH mapping of 30 genetic marker-anchored BACs on the pachytene chromosome 6 of potato.…”

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Chromatin Structure and Physical Mapping of Chromosome 6 of Potato and Comparative Analyses With Tomato

et al. 2008

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Potato (Solanum tuberosum) has the densest genetic linkage map and one of the earliest established cytogenetic maps among all plant species. However, there has been limited effort to integrate these maps. Here, we report fluorescence in situ hybridization (FISH) mapping of 30 genetic marker-anchored bacterial artificial chromosome (BAC) clones on the pachytene chromosome 6 of potato. The FISH mapping results allowed us to define the genetic positions of the centromere and the pericentromeric heterochromatin and to relate chromatin structure to the distribution of recombination along the chromosome. A drastic reduction of recombination was associated with the pericentromeric heterochromatin that accounts for 28% of the physical length of the pachytene chromosome. The pachytene chromosomes 6 of potato and tomato (S. lycopersicum) share a similar morphology. However, distinct differences of heterochromatin distribution were observed between the two chromosomes. FISH mapping of several potato BACs on tomato pachytene chromosome 6 revealed an overall colinearity between the two chromosomes. A chromosome inversion was observed in the euchromatic region of the short arms. These results show that the potato and tomato genomes contain more chromosomal rearrangements than those reported previously on the basis of comparative genetic linkage mapping.

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“…For this approach, genetic mapping data, fluorescence in situ hybridization (FISH), and BAC sequencing analysis provide a framework, in which 84 anchored seed and extension BACs were positioned, covering a total of 2.4 Mb for the short arm and 10.2 Mb for the long arm (our unpublished data). Similarly, a BAC-FISH map has been published for tomato chromosome 1 showing the relation of the linkage map to pachytene chromosome structure (Chang et al 2007). Moreover, a BAC-FISH map is being completed at http://www.sgn.…”

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Cross-Species Bacterial Artificial Chromosome–Fluorescence in Situ Hybridization Painting of the Tomato and Potato Chromosome 6 Reveals Undescribed Chromosomal Rearrangements

et al. 2008

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Ongoing genomics projects of tomato (Solanum lycopersicum) and potato (S. tuberosum) are providing unique tools for comparative mapping studies in Solanaceae. At the chromosomal level, bacterial artificial chromosomes (BACs) can be positioned on pachytene complements by fluorescence in situ hybridization (FISH) on homeologous chromosomes of related species. Here we present results of such a cross-species multicolor cytogenetic mapping of tomato BACs on potato chromosomes 6 and vice versa. The experiments were performed under low hybridization stringency, while blocking with Cot-100 was essential in suppressing excessive hybridization of repeat signals in both within-species FISH and cross-species FISH of tomato BACs. In the short arm we detected a large paracentric inversion that covers the whole euchromatin part with breakpoints close to the telomeric heterochromatin and at the border of the short arm pericentromere. The long arm BACs revealed no deviation in the colinearity between tomato and potato. Further comparison between tomato cultivars Cherry VFNT and Heinz 1706 revealed colinearity of the tested tomato BACs, whereas one of the six potato clones (RH98-856-18) showed minor putative rearrangements within the inversion. Our results present cross-species multicolor BAC-FISH as a unique tool for comparative genetic studies across Solanum species.

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Predicting and Testing Physical Locations of Genetically Mapped Loci on Tomato Pachytene Chromosome1

Cited by 36 publications

References 40 publications

Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

Chromatin Structure and Physical Mapping of Chromosome 6 of Potato and Comparative Analyses With Tomato

Cross-Species Bacterial Artificial Chromosome–Fluorescence in Situ Hybridization Painting of the Tomato and Potato Chromosome 6 Reveals Undescribed Chromosomal Rearrangements

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