Predator performance of Coccinella transversalis and its molecular identification

Abstract

Predatory efficacy of Coccinella transversalis (Coleoptera: Coccinellidae) on three detrimental agriculturally important aphids (Aphis craccivora, A. fabae and A. gossypii) was studied under laboratory condition. The 4 th instar larvae of C. transversalis consumed highest (21.56 ± 1.72) number of A. craccivora aphids followed by A. fabae (12.33 ± 1.74) and A. gossypii (13.99 ± 0.77). Life cycle studies of C. transversalis on the above three aphid species revealed that it took maximum (27.66 ± 3.06) days to complete life cycle while reared on A. craccivora followed by A. fabae (25.66 ± 0.58 days) and A. gossypii (22.33 ± 1.52 days)respectively. As C. transversalis is a potential predator, an attempt was taken to identify this biological control agent based on mitochondrial cytochrome c oxidase (COI) gene sequence. Sequenced gene was submitted to the NCBI GenBank database (Accession NO. MG 587947.1) followed by proper procedure.Phylogenetic relationship of the beetle was constructed based on mitochondrial COI gene. The nucleotide composition analysis revealed that the value of A+T (69.3%) was greater than G+C (30.7%). Such study of C. transversalis would be helpful in biological control programme of aphid pest.

Biology and Predatory Potential of <i>Coccinella septempunctata</i> And <i>C. Transversalis</i> feeding on<i> Lipaphis erysimi</i>

“…The period of first instar grub was in range of 2 to 4 with an average of 2.60± 0.638 days in the present studies. Sarker et al (2019) reported the first instar grub period of C. transversalis were 2.66± 1.15 and 2.66± 0.58 days on A. craccivora and A. gossypii, respectively. Mari et al (2016) reported the period of first instar grub of C. undecimpunctata on mustard aphid, L. erysimi which was 5.23 days.…”

“…Second instar grub period were found to be 2 to 4 with an average of 2.76± 0.655 days. Sarker et al (2019) reported the second instar grub period of C. transversalis 2.66± 0.58 days on A. fabae. The third instar grub period of C. transversalis on mustard aphid, Lipaphis erysimi varied from 2-5 days, with an average of 3.66± 0.831 days in present studies which is in agreement with Sarker et al (2019) who reported the third instar grub period of 3.66± 0.58 days.…”

“…Sarker et al (2019) reported the second instar grub period of C. transversalis 2.66± 0.58 days on A. fabae. The third instar grub period of C. transversalis on mustard aphid, Lipaphis erysimi varied from 2-5 days, with an average of 3.66± 0.831 days in present studies which is in agreement with Sarker et al (2019) who reported the third instar grub period of 3.66± 0.58 days. Rajan et al (2019) reported 3.7± 0.48 days of third instar grub period of C. transversalis on cabbage aphid, B. brassicae.…”

Diversity of insect carried-fungi in chili (Capsicum annuum) crop at Banyumas District, Central Java Province, Indonesia

“…In contrast, E. admirabilis is a pest. C. transversalis is a predator of Aphis gossypii (Glover) (Efendi et al 2018;Sarker et al 2019), A. craccivora, and A. fabae (Sarker et al 2019). No reports have stated that C. transversalis was a vector of a particular pathogen.…”