Predation of colistin- and carbapenem-resistant bacterial pathogenic populations and their antibiotic resistance genes in simulated microgravity

Jang, Hyochan; Choi, Seong Yeol; Mun, Wonsik; Jeong, Seok Hoon; Mitchell, Robert J.

doi:10.1016/j.micres.2021.126941

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…84 In bacteria, SNPs have been associated with the development of antibiotic resistance. 87,88,89,90…”

Section: Discussionmentioning

confidence: 99%

Pharmacogenomics Guided Spaceflight: the intersection between space-flown drugs and space genes

Nelson,

Rose,

Walter

et al. 2024

Preprint

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Ten years ago, it was predicted that the multi-omics revolution would also revolutionize space pharmacogenomics. Current barriers related to the findable, accessible, interoperable, and reproducible use of space-flown pharmaceutical data have contributed to a lack of progress beyond application of earth-based principles. To directly tackle these challenges, we have produced a novel database of all the drugs flown into space, compiled from publicly available ontological and spaceflight-related datasets, to exemplify analyses for describing significant spaceflight-related targets. By focusing on mechanisms perturbed by spaceflight, we have provided a novel avenue for identifying the most relevant changes within the drug absorption, distribution, metabolism, and excretion pathways. We suggest a set of space genes, by necessity limited to available tissue types, that can be expanded and modified based on future tissue-specific and mechanistic-specific high-throughput assays. In sum, we provide the justification and a definitive starting point for pharmacogenomics guided spaceflight as a foundation of precision medicine, which will enable long-term human habitation of the Moon, Mars, and beyond.

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“…84 In bacteria, SNPs have been associated with the development of antibiotic resistance. 87,88,89,90…”

Section: Discussionmentioning

confidence: 99%

Pharmacogenomics Guided Spaceflight: the intersection between space-flown drugs and space genes

Nelson,

Rose,

Walter

et al. 2024

Preprint

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“…Although colistin is the last resort for clinical treatment of multi-drug resistant Gram-negative bacteria infection, the continuous emergence of colistin-resistant bacteria posed a serious threat to the efficacy of antibiotic therapy ( Laplante et al, 2018 ; Huang et al, 2021 ; Jang et al, 2021 ). In addition, bacteria can be adapted to adverse and detrimental conditions through stress-triggered regulatory systems ( Yin et al, 2021 ; Dawan and Ahn, 2022 ), such as TCS, which has increased the difficulty of prevention and control of pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Contribution of the EnvZ/OmpR two-component system to growth, virulence and stress tolerance of colistin-resistant Aeromonas hydrophila

Gang

Zheng

et al. 2022

Front. Microbiol.

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Aeromonas hydrophila is an important zoonotic pathogen responsible for septicemia, diarrhea and gastroenteritis, and has attracted considerable attention. The EnvZ/OmpR two-component system (TCS) mediates environmental stress responses in gram-negative bacteria. We investigated the role of the TCS in A. hydrophila by comparing the characteristics of the parental (23-C-23), EnvZ/OmpR knockout (23-C-23:ΔEnvZ/OmpR), and complemented strains (23-C-23:CΔEnvZ/OmpR). Under non-stress conditions, the 23-C-23:ΔEnvZ/OmpR strain showed a significant decrease in growth rate compared to that of 23-C-23. Transcriptome and metabonomic analysis indicated that many metabolic pathways were remarkably affected in the ΔEnvZ/OmpR strain, including the TCA cycle and arginine biosynthesis. In addition, the virulence of the ΔEnvZ/OmpR strain was attenuated in a Kunming mouse model. The ΔEnvZ/OmpR strain exhibited notably reduced tolerance to environmental stresses, including high temperature, different pH conditions, oxidative stress, and high osmotic stress. The downregulated expression of genes related to cell metabolism, motility, and virulence in the ΔEnvZ/OmpR mutant strain was further validated by real-time quantitative PCR. Consequently, our data suggest that the EnvZ/OmpR TCS is required for growth, motility, virulence, and stress response in A. hydrophila, which has significant implications in the development of novel antibacterial and vaccine therapies targeting EnvZ/OmpR against A. hydrophila.

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“…The best-characterized BALOs employ an intraperiplasmic growth phase, where they enter the periplasm of the prey and secrete numerous hydrolytic enzymes ( 3 , 4 ). These enzymes hydrolyze the prey’s proteins and nucleic acids ( 5 , 6 ) to generate the monomers necessary for the BALOs’ growth and replication. Although research during the last 2 decades has done much to unravel the life cycle of BALOs, many mysteries still surround these predators, including how these predators recognize their prey.…”

Section: Observationmentioning

confidence: 99%

Outer Membrane Porin F in E. coli Is Critical for Effective Predation by Bdellovibrio

et al. 2022

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Predation of colistin- and carbapenem-resistant bacterial pathogenic populations and their antibiotic resistance genes in simulated microgravity

Cited by 13 publications

References 27 publications

Pharmacogenomics Guided Spaceflight: the intersection between space-flown drugs and space genes

Pharmacogenomics Guided Spaceflight: the intersection between space-flown drugs and space genes

Contribution of the EnvZ/OmpR two-component system to growth, virulence and stress tolerance of colistin-resistant Aeromonas hydrophila

Outer Membrane Porin F in E. coli Is Critical for Effective Predation by Bdellovibrio

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