Precursor-product relationship between rabbit type II cell lamellar bodies and alveolar surface-active material

Baritussio, Aldo; Magoon, Martha W.; Goerke, Jon; Clements, John A.

doi:10.1016/0005-2760(81)90297-6

Cited by 100 publications

(36 citation statements)

References 39 publications

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“…Additional factors, other than surfactant, may influence neonatal lung stability. These include alveolar capillary permeability (34), composition and microstructure of alveolar surfactant (35) or the connective tissue of the lung (36)(37)(38). In our study, inosito1-k glucocorticoid increased lavageable surfactant in the fetus.…”

Section: Resultsmentioning

confidence: 48%

Inositol and Glucocorticoid in the Development of Lung Stability in Male and Female Rabbit Fetuses

et al. 1988

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ABSTRACT. Inasmuch as inositol affects the development of lung surfactant, and exogenous glucocorticoids accelerate fetal lung maturation, a possible interaction of the two substances on alveolar stability of preterm rabbit fetuses of 28 days gestation was investigated. On days 26 and 27 of gestation inositol or glucose were added to the diet of does treated with betamethasone (0.2 mg/kg intramuscularly on days 26 and 27). Inositol increased lung-thorax compliance of paralyzed fetuses at all insufflation pressures studied (from 16 to 22.5 and 30 cm HzO and back to 22.5, and 16 cm HzO). At a ventilation pressure of 30 cm HzO, lung-thorax compliance of fetuses treated with inosit01 plus betamethasone was more than doubled as compared with controls (1.2 2 0.6 versus 0.5 f 0.2 ml/kg X cm HzO,p < 0.001). Inositol alone had no detectable effect on compliance, whereas betamethasone tended to increase compliance (p = 0.05). According to variance analysis, the effect of inositol was statistically significant only among the males. Inositol prevented the glucocorticoid-induced decrease in lung protein and, to a lesser extent, the decrease in DNA. Inositol did not further increase the lavageable surfactant pool of the glucocorticoid-treated, ventilated fetuses, although the area occupied by lamellar bodies within type I1 cells was increased after inositol plus betamethasone. According to the present study, inositol modifies the physiologic and biochemical response of the immature fetal lung to a pharmacologic dose of exogenous glucocorticoid. (Pediatr Res 24: 617-621, 1988) Abbreviations BM, betamethasone BW, body weight RDS, respiratory distress syndromeThe physiologic role of glucocorticoids in fetal lung maturation is widely acknowledged (1, 2). Exogenous glucocorticoids accel-

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Section: Resultsmentioning

confidence: 48%

Inositol and Glucocorticoid in the Development of Lung Stability in Male and Female Rabbit Fetuses

et al. 1988

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“…2) According to current evidence there is a bidirectional flux of intact surfactant phospholipids between alveolar space and the lamellar bodies (12)(13)(14). In a 3-day rabbit about 90% of the phosphatidylcholine secretion from lamellar bodies to alveolar lining is likely to be due to reutilization of intact phospholipids.…”

Section: Discussionmentioning

confidence: 99%

“…According to animal studies there is a rapid bidirectional flux of intact surfactant phospholipids between intracellular lamellar bodies and the surface of the airways (1 [2][3][4][5][6][7][8][9][10][11][12][13][14]. These interactions between intra-and extracellular surfactant can be about one order of magnitude faster than the apparent rate of surfactant synthesis and secretion (1 3).…”

mentioning

confidence: 99%

Effect of Surfactant Substitution on Lung Effluent Phospholipids in Respiratory Distress Syndrome: Evaluation of Surfactant Phospholipid Turnover, Pool Size, and the Relationship to Severity of Respiratory Failure

et al. 1986

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ABSTRACT. The turnover and pool size of surfactant has Abbreviations been studied in animals, but there is little similar information in humans. In the present investigation lung effluent phospholipids were studied in 29 small preterm infants with severe RDS. Thirteen were treated with mechanical ventilation, and 16 additionally received natural human surfactant. The first dose (60 mg surfactantlkg body wt) was given between 2 and 10 h of age, and the surfactant was given again if there was an insufficient response. Together 260 aspirates, recovered during routine suctioning of the airways, were analyzed for phospholipids. Phosphatidylglycerol, present only in exogenous surfactant, was used as a specific marker to estimate the apparent pool size and the half-life of surfactant phospholipid. In addi-.tion, the saturated phosphatidylcholine/sphingomyelin ratios were correlated with the ventilatorv index (mean airway pressure X fractional inspiratory oxygen/arterial oxygen tension). There was a linear correlation between the ventilatory index and the saturated phosphatidylcholine/ sphingomyelin ( r --0.70) but no consistent correlation between the ventilatory index and the amount of phospholipids in the aspirate. The saturated phosphatidylcholine/ sphingomyelin ratio increased during the surfactant-induced remission of respiratory failure, decreased during the relapse of respiratory failure (present among 50% of the surfactant-treated infants), and increased again during the recovery. The control infants tended to have lower saturated phosphatidylcholine/sphingomyelin ratios during the first week than the surfactant-treated infants. Therecipients of surfactant had slightly more severe lung disease than the controls, when the results were adjusted by covariance to remove the differences in the saturated phosphatidylcholine/sphingomyelin ratio. Exogenous surfactant increased the amarent endogenous ~o o l size at least fivefold. The apparent half-life of ~hosph~tidylglycerol was 30 h (20-36 h). The half-life was independent of the amount of exogenous surfactant (60 versus 120 n~g/kg). Therefore, the apparent turnover rate after 120 was higher than after 60 mg/kg surfactant (p < 0.01).

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“…Synthesis and secretion of surfactant lipids is known to occur at a rapid rate with a turnover time for DPPC of 10.7 h in the rabbit (30) and a biological half-life in the rat of -4 h (10). The process of clearance of the secreted phospholipids is less well understood.…”

Section: Discussionmentioning

confidence: 99%

An ethanol/ether soluble apoprotein from rat lung surfactant augments liposome uptake by isolated granular pneumocytes.

Claypool¹,

Wang²,

Chander³

et al. 1984

J. Clin. Invest.

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bstract. Ethanol/ether soluble apoproteins, comprising 17% of the total recovered surfactant-associated proteins, were isolated from rat lung surfactant and purified by silicic acid chromatography. The protein that eluted in 4:1 chloroform/methanol accounted for >85% of protein in the ethanol/ether soluble fraction and was termed surfactant apoprotein Et (Apo Et). By sodium dodecyl sulfate polyacrylamide gel electrophoresis, this protein had an apparent molecular weight of -10,500. Apo Et was evaluated for its effect on uptake of synthetic phospholipids in liposomal form by isolated granular pneumocytes (Type II alveolar epithelial cells) in primary culture. Liposomes were prepared to approximate the phospholipid composition of the aveolar surfactant, and uptake was measured by the accumulation of the radioactively labeled dipalmitoyl phosphatidyl choline fraction. The uptake of liposomal phosphatidylcholine by cells incubated for 2 h with Apo Et was increased by 61% over control. Most of the cell-associated phospholipid uptake was resistant to treatment with trypsin, suggesting an increased internalization of liposomal material in the presence of Apo Et. The effect of Apo Et on uptake was concentration and time dependent and was not associated with cell damage,

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Precursor-product relationship between rabbit type II cell lamellar bodies and alveolar surface-active material

Cited by 100 publications

References 39 publications

Inositol and Glucocorticoid in the Development of Lung Stability in Male and Female Rabbit Fetuses

Inositol and Glucocorticoid in the Development of Lung Stability in Male and Female Rabbit Fetuses

Effect of Surfactant Substitution on Lung Effluent Phospholipids in Respiratory Distress Syndrome: Evaluation of Surfactant Phospholipid Turnover, Pool Size, and the Relationship to Severity of Respiratory Failure

An ethanol/ether soluble apoprotein from rat lung surfactant augments liposome uptake by isolated granular pneumocytes.

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