Preconditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability

Hosomi, Silvério Takao; Santos, Reginaldo Bezerra dos; Custódio, Ceci Castilho; Seaton, Philip T.; Marks, T. R.; Neto, Nelson Barbosa Machado

doi:10.15258/sst.2011.39.1.15

Cited by 47 publications

(43 citation statements)

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“…Many preconditioning treatments have been used in seeds of forest species, such as cutting, scarification and soaking in water (Oliveira et al, 2001;Mendonça et al, 2006). According to Hosomi et al, (2011), the comparison between in vitro germination rates of various species of the genus Cattleya with the results obtained by tetrazolium test applied to seeds submitted to preconditioning in a 10% sucrose solution, showed similar viability rates, demonstrating the importance of using sucrose solution for preconditioning. Custódio et al (2016) obtained similar results for Dactylorrhyza and Vanda; the same has been described for Aerides maculosum (Deepti et al, 2013) and Aerides ringens (Deepti et al, 2015).…”

Section: Introductionmentioning

confidence: 89%

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Hosomi¹,

Souza²,

Custódio³

et al. 2017

Aust J Crop Sci

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The evaluation of seed physiological quality can help taking quick decision about the use or disposal of lots for conservation. The tetrazolium test is one of the most traditional in the analysis of seeds quality. Optional preconditioning procedures have improved the efficiency of this test. This work compared the efficiency of different preconditioning procedures, the buffered solution, concentrations and exposure time to tetrazolium solution in assessing the quality of orchid seeds of Cattleya labiata and Cattleya tigrina lots. The analysis was made through magnification of digital images. For preconditioning evaluation, two seed lots were aliquoted and submitted to different treatments: 10% sucrose solution, 10% glucose solution and water at room temperature for 24 hours. After preconditioning, seeds were immersed in tetrazolium solutions -prepared with buffer or water -at concentrations of 0.1, 0.5 and 1.0% for periods of 3, 12 and 24 hours, at 40 °C, in a water bath, in the dark. The experiment design was arranged in a factorial 4x3x3x2 and the mean comparison was made by Tukey test at 5% probability. Tetrazolium test in preconditioned orchid seeds allowed better visualization of red viable seeds. Tetrazolium test in orchid seeds preconditioned in glucose 10% solution or sucrose 10% solution was more effective for viable seeds visualization. Diluting tetrazolium salt in buffered solution or water made no difference for the results. Maintaining seeds in contact with tetrazolium 1% solution for 24 hours provided the higher viability values for both seed lots.

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Section: Introductionmentioning

confidence: 89%

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Hosomi¹,

Souza²,

Custódio³

et al. 2017

Aust J Crop Sci

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“…Jamun seeds are known to be recalcitrant, chlorotic, multicotyledonous, and have multiple embryos, although there have been very few studies examining their seed anatomy or directed towards improving seed germination and vigor (Swamy et al 1999, Thoke et al 2009). The tetrazolium (TZ) test is one of the most reliable techniques used to estimate seed viability and vigor (Hosomi et al 2011), although every species has its own specifi c dissection, staining, and TZ evaluation requirements (Enescu 1991). The present investigation was conducted to: 1. assess the fruit:seed and seed: seed coat, cotyledon and embryo ratios; 2. examine the polyembryonic nature of the seeds; 3. elucidate the infl uence of the seed coat on seed germination and; 4. determine the topographical staining patterns of jamun seeds.…”

Section: Introductionmentioning

confidence: 99%

Viability and vigor of jamun (Syzygium cumini) seeds

Sivasubramaniam¹,

Selvarani²

2012

Braz. J. Bot.

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-(Viability and vigor of jamun (Syzygium cumini) seeds). Jamun (Syzygium cumini L. Skeels) (Black plum, Damson plum) fruits weigh between 2-5 g at maturity. Fresh seeds represented 20-80% of the total fruit weight; the seed coat and cotyledons contributed 6% and 94% to the total seed weight respectively, while the weight of the embryonic axis was insignifi cant. Only the embryonic axis stained with Tetrazolium, not the cotyledons. The seeds are polyembryonic with up to four embryos, of which at most three germinate. Decoated seeds germinated faster than coated seeds under nursery conditions, with high signifi cant germination percentages, dry matter production rates and vigor indices. The lack of staining of the cotyledon by tetrazolium was probably due to the presence of an impermeable layer. Decoating seeds for faster germination is recommended.

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“…Sob este aspecto, a micropropagação proporcionou resultados expressivos com outras espécies esterilizados em autoclave a 121 °C e 1,1 atmosfera durante 15 minutos (Caldas et al 1998). Outra amostra de 10 mg de sementes foi utilizada para avaliação da viabilidade pelo teste de tetrazólio, conforme protocolo descrito por Hosomi et al (2011) e, a partir de diluições seriadas, três amostras de aproximadamente 1000 sementes foram observadas. Apenas sementes que apresentaram coloração rosa-avermelhadas foram contabilizadas como viáveis e assim obtido o percentual de viabilidade.…”

Section: Methodsunclassified

Desenvolvimento inicial e crescimento in vitro de Cattleya violacea (Kunth) Rolfe em diferentes concentrações de sacarose

Júnior

Mantovani

Cassano³

et al. 2013

Acta Amaz.

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RESUMOO presente estudo teve por objetivo analisar os aspectos da germinação e avaliar o efeito de concentrações de sacarose no crescimento in vitro de Cattleya violacea. Sementes provenientes de cápsulas fechadas foram semeadas em meio de cultura Murashige e Skoog (MS) e a morfologia externa da semente à plântula foi fotodocumentada em estereomicroscópio e microscópio eletrônico de varredura. Plântulas com 90 dias após a semeadura foram repicadas em meio de cultura ½ MS (com metade da concentração de macronutrientes) com diferentes concentrações de sacarose (0, 10, 20, 30 e 40 g L -1 ), incubadas nas mesmas condições in vitro por mais 150 dias e em seguida as plântulas foram avaliadas quanto ao número de raízes, comprimento da maior raiz, número de folhas, comprimento da parte aérea, massa fresca e seca total. Os dados biométricos foram submetidos à análise estatística e a eles ajustadas curvas de regressão. As sementes apresentaram testa reticulada com uma extremidade micropilar (aberta) e calazal (fechada); o embrião originou uma estrutura tuberiforme clorofilada denominada protocormo que pode apresentar rizóides, folíolos e quando provido de raiz é considerado plântula. A ausência de açúcar ou a maior concentração avaliada de sacarose foram prejudiciais ao crescimento da planta. A concentração de 27 g L -1 proporcionou maior crescimento in vitro possibilitando maior eficiência para a propagação massal dessa espécie de elevado potencial ornamental. PALAVRAS-CHAVE: Germinação assimbiótica, orquídea nativa, carboidrato. Initial development and in vitro growth of Cattleya violacea (Kunth) Rolfe in different sucrose concentrations ABSTRACTThe aims of this study were to analyze the initial germination aspects and evaluate the effect of sucrose concentrations on in vitro growth of Cattleya violacea. Mature seeds from closed pods were sowed on MS (Murashige and Skoog) culture medium. The external morphology of seed and plantlets were documented by light and scanning electron microscopy. Plantlets with 90 days after sowing were subcultured on ½ MS (with half-strength macronutrients concentrations) culture medium with different sucrose concentrations (0, 10, 20, 30 and 40 g L -1 ), incubated under the same in vitro conditions during more 150 days followed by the evaluation of root number, root length, shoot length, fresh and dry weight. The biometric data were statistically analyzed and regression curves constructed. The seeds showed reticulate seed coat with a micropylar (opened) and chalazal (closed) end; the embryo results a chlorophyllated tuberiforme structure called protocorm, which may have rhizoids, leaflets and is considered plantlet when it has root. Absence of sucrose or the highest evaluated sucrose concentration was detrimental for plant growth. The concentration of 27 g L -1 provided the highest in vitro growth enabling great efficiency for mass propagation of this species of high ornamental potential.

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Preconditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability

Cited by 47 publications

References 0 publications

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Viability and vigor of jamun (Syzygium cumini) seeds

Desenvolvimento inicial e crescimento in vitro de Cattleya violacea (Kunth) Rolfe em diferentes concentrações de sacarose

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