Preclinical hematological profile studies of an ayurvedic medicine Siddha Makardhwaja after chronic administration to male sprague-dawley rats

Abstract

Background: Siddha Makardhwaja (SMD) is a classical Ayurvedic formulation markedly used as a traditional medicine in the rural population for various purposes such as stimulant, tonic, and rejuvenator.Methods: The present study is conducted to evaluate the effect of conventionally prepared SMD on the hematological parameters in experimental animals, for providing scientific data base for its logical use in clinical practice. Acute toxicity tests were conducted to determine the LD50 of the drug. To find out the effect of chronic administration of SMD on hematological parameters it was administered chronically to the male Sprague-Dawley rats at a dose of 40mg/kg for 28 days.Results: In this experiment the TC, DC, various erythrocytic parameters, platelet parameters, ESR were determined. The results of the studies are given below. There is an (13.41%) increase in the number of white blood cell count of the male rat, the increase though not significant yet it was prominent (p=0.257). There is an (15.87%) increase in the absolute count of Neutrophils of the male rat, the increase though not significant yet it was prominent (p=0.371). There is an (12.29%) increase in the absolute count of Lymphocytes of the male rat, the increase though not significant yet it was prominent (p=0.388). There is a statistically significant (p=0.035) increase in the number of platelet count of the male rat (11.13% increase). There is a (2.03%) decrease in the platelet volume distribution width of the male rat, the decrease though not significant yet it was noticeable (p=0.094). There is a statistically insignificant (p=0.619) (10.0%) increase in Erythrocyte sedimentation rate in blood from the male rat.Conclusions: As SMD decreases and increases abnormally on the hematological parameters in body of treated rats, so it should not be administered chronically at a higher dose. Further studies should be done by reducing the administered dose.

Hematological Studies of an Ayurvedic Medicine “Vata Gajendra Singha” Used in Rheumatoid Arthritis After Chronic Administration to Male Sprague-Dawley Rats

“…The neutrophil increase can occur due to several mechanisms, such as a shift from marginating to circulating pool, increased release from bone marrow, increased production by the bone marrow, and decreased migration into tissue. Neutrophils too high, known as neutrophilia, 29 may cause acute bacterial infections, inflammation, and tissue death. Low lymphocyte concentration is associated with increased infection rates after surgery or trauma.…”

“…Low lymphocyte concentration is associated with increased infection rates after surgery or trauma. Lymphocytes too low, known as lymphocytopenia 29 may cause autoimmune disorders such as rheumatoid arthritis and bone marrow damage. The value of Mean Corpuscular Volume (MCV) highly significantly decreased (<p=.01) in low dose and very highly significantly decreased (<p=.001) in high dose to the VGS treated rats in our study (Table 3).…”

“…MCH test helps diagnose the type of anemia. 29 There are several reasons that can contribute to a person having a low MCH level, such as blood loss, folic acid, and or Vitamin B12 deficiency. The value of Mean Corpuscular Hemoglobin (MCH) significantly decreased (<p=.05) in both doses in the VGS-treated rats (Table 3).…”

Steroidal and gonadotropin hormone profile studies of a classical ayurvedic preparation of “Saraguna Balijarita Makardhwaja” after chronic administration to male Sprague-Dawley rats

“…The possible toxicity of certain chemicals is identified and also proper guidelines are provided for their safe usage from drug safety profile. [34][35][36][37] Like any other type of treatment, ayurvedic medicine has the potential for harm if not used properly. It is reported from a study that heavy metals such as mercury, lead, arsenic, and chromium were present in the majority of Ayurvedic formulations.…”

Ayurvedic Medicine: A Traditional Medical System and Its Heavy Metal Poisoning

“…Some herbs, if not used appropriately, can show toxic effects ( Table 1 ). 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Ayurvedic texts classify herbs according to their safety and potential toxicity. The “Bhavaprakasha Nighantu” and other Ayurvedic pharmacopeias provide information about the toxicity, dosage, and contraindications of various herbs.…”