Preclinical and histological study of boron-containing compounds hydrogels on experimental model of periodontal disease

Abstract

Periodontitis is a disease that affects a wide group of people, and there has been an increased interest in the research of finding useful materials that help reduce inflammation and the further loss of tissue. In this study, we have tested a boron-containing compound (BCC) Calcium Fructoborate (CaFB) and Boric Acid (BA) hydrogels on the gingival level on Wistar rats. First, we have induced the periodontal disease at the lower incisors, we have applied the hydrogels and after a week, we have euthanized the rats. Next, the oral soft tissue reaction was clinically and then histologically investigated. Our study has shown good clinical response of the oral tissue, and we have noticed lower levels of inflammation on the experimental groups treated with the BCCs hydrogels. Despite the generally good response of the biological structures to the presence of BA and CaFB on periodontal level, more scientifically proved information is needed to obtain the desired biological responses in all clinical situations.

New Insights into Boron Essentiality in Humans and Animals

“…( j ) B in oral washes positively influences periodontal health. BA and calcium fructoborate (CaFB) hydrogels have shown their potential as a treatment option for gingivitis and periodontitis [ 77 ]. In another study, the B levels in non-carious teeth were also higher than in carious teeth, and a significant negative correlation was identified only in non-carious teeth group [ 78 ].…”

“…At the same time, the ability to buffer saliva increased significantly after a diet rich in B, and the B level of decayed teeth was lower than that of healthy teeth [ 81 ]. Increased B in saliva has a positive effect on dental and oral health and may decrease the formation of cavities and show potential as a treatment option for gingivitis and periodontitis [ 77 ]. Important changes in salivary buffering capacity and TSH during a natural B-rich diet are of clinical importance, with dysbiosis being a common finding in thyroid disorders [ 81 ].…”

“…Important changes in salivary buffering capacity and TSH during a natural B-rich diet are of clinical importance, with dysbiosis being a common finding in thyroid disorders [ 81 ]. Recent work has also revealed that B-rich foods result in cardioprotective effects and longevity, improving long-term survival among patients with kidney transplantation (KTR) [ 77 ]. Another recent work showed that B-rich foods result in lower mortality risks and a more favorable cardiometabolic risk profile [ 82 ].…”

“…Our 30-year research has attempted to fill this scientific gap by studying the many chemical and biochemical reactions in which B is involved [ 3 , 4 , 18 , 34 , 35 , 41 , 77 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 ]. Recently, we analyzed some of our old animal studies and found that at the beginning of the B diet, the increase in the B concentration in feces could be explained by NOB species’ indigestibility; then, NOB is released into the colon, where the microbiota needs it [ 135 , 136 , 137 , 138 ].…”

“…The literature explains that the health of humans/animal organisms depends on the health of the microbiota [ 116 , 149 ], supporting the view that the role of NOB species is crucial for the health of the human/animal organism [ 34 ]. This role has resulted from many scientific experiments shown that NOB species increase the buffering capacity of saliva [ 77 , 81 ], have a positive impact on the intestinal and oral microbiome [ 76 ], protect important probiotic bacteria, namely Bifidobacterium spp. and Lactobacillus spp.…”