Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator

Shemesh, Or A.; Linghu, Changyang; Piatkevich, Kiryl D.; Goodwin, Daniel; Celiker, Orhan T.; Gritton, Howard J.; Romano, Michael F.; Gao, Ruixuan; Yu, Chih Chieh; Tseng, Hua An; Bensussen, Seth; Narayan, Sujatha; Yang, Chao; Freifeld, Limor; Siciliano, Cody A.; Gupta, Ishan; Wang, Joyce; Pak, Nikita; Yoon, Young Gyu; Ullmann, Jeremy F.P.; Guner-Ataman, Burcu; Noamany, Habiba; Sheinkopf, Zoe R.; Park, Won Min; Asano, Shoh; Keating, Amy E.; Trimmer, James S.; Reimer, Jacob; Tolias, Andreas S.; Bear, Mark F.; Tye, Kay M.; Han, Xue; Ahrens, Misha B.; Boyden, Edward S.

doi:10.1016/j.neuron.2020.05.029

Cited by 100 publications

(65 citation statements)

References 68 publications

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“…LNvs receive synaptic input from larval photoreceptors and exhibit robust calcium responses upon light stimulation 41 . Using a genetically encoded calcium indicator GCaMP7f 50 , we measured light-evoked calcium increases in the axonal terminal region of the LNvs (Fig. 3d ).…”

Section: Resultsmentioning

confidence: 99%

Brain-specific lipoprotein receptors interact with astrocyte derived apolipoprotein and mediate neuron-glia lipid shuttling

et al. 2021

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Lipid shuttling between neurons and glia contributes to the development, function, and stress responses of the nervous system. To understand how a neuron acquires its lipid supply from specific lipoproteins and their receptors, we perform combined genetic, transcriptome, and biochemical analyses in the developing Drosophila larval brain. Here we report, the astrocyte-derived secreted lipocalin Glial Lazarillo (GLaz), a homolog of human Apolipoprotein D (APOD), and its neuronal receptor, the brain-specific short isoforms of Drosophila lipophorin receptor 1 (LpR1-short), cooperatively mediate neuron-glia lipid shuttling and support dendrite morphogenesis. The isoform specificity of LpR1 defines its distribution, binding partners, and ability to support proper dendrite growth and synaptic connectivity. By demonstrating physical and functional interactions between GLaz/APOD and LpR1, we elucidate molecular pathways mediating lipid trafficking in the fly brain, and provide in vivo evidence indicating isoform-specific expression of lipoprotein receptors as a key mechanism for regulating cell-type specific lipid recruitment.

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Section: Resultsmentioning

confidence: 99%

Brain-specific lipoprotein receptors interact with astrocyte derived apolipoprotein and mediate neuron-glia lipid shuttling

et al. 2021

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“…For example, red Ca 2+ sensors R-GECO [ 86 , 87 ] and RCaMP [ 87 , 88 ] were developed from cp-mApple and cp-mRuby, respectively. The Ca 2+ sensors were continuously improved; thus, we can currently monitor dynamic changes of intracellular Ca 2+ levels in living animal as well as live cells [ 89 , 90 , 91 , 92 , 93 ].…”

Section: Sensing Strategies Of Fp-based Biosensorsmentioning

confidence: 99%

Genetically Encoded Biosensors Based on Fluorescent Proteins

Kim

Lee

et al. 2021

Sensors

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Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs.

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“…In these in vivo instances, using confocal laser scanning microscopy would have likely been inadequate, as comparatively, 2PI significantly reduces photobleaching, increases cell viability, and allows for deeper tissue penetration [69]. Furthermore, it has been reported that, when imaging neuronal cells specifically, single-photon microscopy suffers from increased interference by neuropil and decreased signal-to-noise ratio compared to 2PI [70].…”

Section: Two-photon Imagingmentioning

confidence: 99%

Two-Photon Imaging to Unravel the Pathomechanisms Associated with Epileptic Seizures: A Review

et al. 2021

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Despite extensive research, the exact pathomechanisms associated with epileptic seizure formation and propagation have not been elucidated completely. Two-photon imaging (2PI) is a fluorescence-based microscopy technique that, over the years, has been used to evaluate pathomechanisms associated with epileptic seizures and epilepsy. Here, we review previous applications of 2PI in epilepsy. A systematic search was performed in multiple literature databases. We identified 38 publications that applied 2PI in epilepsy research. These studies described models of epileptic seizure propagation; anatomical changes and functional alterations of microglia, astrocytes, and neurites; and neurometabolic effects that accompany seizures. Moreover, various neurovascular alterations that accompany seizure onset and ictal events, such as blood vessel responses, have been visualized using 2PI. Lastly, imaging and quantitative analysis of oxidative stress and the aggregation of lipofuscin in the neurovasculature have been accomplished with 2PI. Cumulatively, these papers and their reported findings demonstrate that 2PI is an especially well-suited imaging technique in the domain of epilepsy research, and these studies have significantly improved our understanding of the disorder. The application of 2PI provides ample possibilities for future research, most interestingly on human brains, while also stretching beyond the field of epilepsy.

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Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator

Cited by 100 publications

References 68 publications

Brain-specific lipoprotein receptors interact with astrocyte derived apolipoprotein and mediate neuron-glia lipid shuttling

Brain-specific lipoprotein receptors interact with astrocyte derived apolipoprotein and mediate neuron-glia lipid shuttling

Genetically Encoded Biosensors Based on Fluorescent Proteins

Two-Photon Imaging to Unravel the Pathomechanisms Associated with Epileptic Seizures: A Review

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