Precipitation of calcium carbonate and calcium phosphate under diffusion controlled mixing

Gebrehiwet, T.; Guo, Luanjing; Fox, D. T.; Huang, Hai; Fujita, Yoshiko; Smith, Robert W.; Henriksen, James R.; Redden, G. D.

doi:10.1016/j.apgeochem.2014.04.005

Cited by 20 publications

(12 citation statements)

References 71 publications

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“…DDC-SEM images similarly suggested a lack of close association between less dense matrix and denser mineral in DBeQ-treated cultures. Moreover, high magnification secondary electron SEM images of DBeQ-treated cultures revealed needle-like mineral, akin to that observed in non-physiological precipitation reactions [ 70 ]. These observations suggest that in addition to an aberrant cell-mediated mineralisation process, the more crystalline mineral in DBeQ-treated cultures could have been formed in a pure physicochemical process, similar to that in pathological calcifications [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties

et al. 2018

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Cellular function depends on the maintenance of protein homeostasis (proteostasis) by regulated protein degradation. Chronic dysregulation of proteostasis is associated with neurodegenerative and age-related diseases, and drugs targeting components of the protein degradation apparatus are increasingly used in cancer therapies. However, as chronic imbalances rather than loss of function mediate their pathogenesis, research models that allow for the study of the complex effects of drugs on tissue properties in proteostasis-associated diseases are almost completely lacking. Here, to determine the functional effects of impaired proteostatic fine-tuning, we applied a combination of materials science characterisation techniques to a cell-derived, in vitro model of bone-like tissue formation in which we pharmacologically perturbed protein degradation. We show that low-level inhibition of VCP/p97 and the proteasome, two major components of the degradation machinery, have remarkably different effects on the bone-like material that human bone-marrow derived mesenchymal stromal cells (hMSC) form in vitro. Specifically, whilst proteasome inhibition mildly enhances tissue formation, Raman spectroscopic, atomic force microscopy-based indentation, and electron microscopy imaging reveal that VCP/p97 inhibition induces the formation of bone-like tissue that is softer, contains less protein, appears to have more crystalline mineral, and may involve aberrant micro- and ultra-structural tissue organisation. These observations contrast with findings from conventional osteogenic assays that failed to identify any effect on mineralisation. Taken together, these data suggest that mild proteostatic impairment in hMSC alters the bone-like material they form in ways that could explain some pathologies associated with VCP/p97-related diseases. They also demonstrate the utility of quantitative materials science approaches for tackling long-standing questions in biology and medicine, and could form the basis for preclinical drug testing platforms to develop therapies for diseases stemming from perturbed proteostasis or for cancer therapies targeting protein degradation. Our findings may also have important implications for the field of tissue engineering, as the manufacture of cell-derived biomaterial scaffolds may need to consider proteostasis to effectively replicate native tissues.

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Section: Discussionmentioning

confidence: 99%

An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties

et al. 2018

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“…in sediments and soils (Sánchez-Pastor et al, 2011, and references therein), where fluids are trapped in a network of interconnected pores (e.g. Putnis et al, 1995;Gebrehiwet et al, 2014). Furthermore, silica hydrogel experiments are considered suitable to mimic crystallization conditions during biomineralization processes (Asenath-Smith et al, 2012).…”

Section: Incorporation Of Chromium Into Calcitementioning

confidence: 98%

“…However, the large crystal size reached by silica hydrogel precipitates is an important advantage that allows for hand-picking of selected CaCO 3 polymorphs. Recent efforts that were aimed at resolving the evolution of physicochemical conditions within silica hydrogel systems prior and during crystallization (Sancho-Tomás et al, 2013;Gebrehiwet et al, 2014) have greatly facilitated a more accurate timing and spatial understanding of the conditions needed for initiation of crystallization, further allowing to implement simple algorithms in geochemical codes (PHREEQC, Parkhurst and Appelo, 1999). However, despite these improvements, it is still not possible to give a quantitative account of their evolution once the first crystals start to grow and new crystals simultaneously form in other places of the precipitation region.…”

Section: Incorporation Of Chromium Into Calcitementioning

confidence: 99%

Fractionation behavior of chromium isotopes during coprecipitation with calcium carbonate: Implications for their use as paleoclimatic proxy

Rodler

Sánchez-Pastor

Fernández-Dı́az

et al. 2015

Geochimica et Cosmochimica Acta

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“…Carbonate ions (CO3 2-) or hydrogen carbonate ions (HCO3 -) are also necessary for the precipitation of CaCO3 or CaMg(CO3)2 (Gebrehiwet et al 2014;Kemache et al 2016). Usually, CO3 2or HCO3ions are present in water.…”

Section: Discussionmentioning

confidence: 99%

Precipitation of calcium compounds onto rock surfaces in water with cementitious material

Nara

Kashiwaya

Nakao

et al. 2018

Carbonates Evaporites

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In this study, the precipitation of minerals onto rock surfaces was investigated to consider whether sealing of pores and cracks in rock can be accelerated. Cylindrical specimens were prepared and then kept in purified water with powders of high-strength and ultra-low-permeability concrete (HSULPC), which will be used to confine transuranic (TRU) wastes in Japan. Then, the rock specimens were weighed and the surfaces of rock specimens were inspected under a microscope. It was recognized that precipitation occurred on the surface of the rock specimens. It was also shown that precipitation did not occur on rock specimens kept in water without HSULPC. The weight of all specimens stored in HSULPC increased, and the observed weight change was larger for rocks with higher porosities. It is concluded that precipitation of minerals occurs on the rock surface when the rock is kept in water with HSULPC powders. From the results obtained in this study, it is suggested that the sealing of pores and cracks in rock can be accelerated by the precipitation of calcium compounds using HSULPC. It is concluded that HSULPC is useful for underground radioactive waste disposal.

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Precipitation of calcium carbonate and calcium phosphate under diffusion controlled mixing

Cited by 20 publications

References 71 publications

An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties

An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties

Fractionation behavior of chromium isotopes during coprecipitation with calcium carbonate: Implications for their use as paleoclimatic proxy

Precipitation of calcium compounds onto rock surfaces in water with cementitious material

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