This research aims to measure the practicality of interactive e-Modules based on 21st century skills in Science Learning Courses for Early Childhood which have been developed by researchers through development research using the ADDIE model. The practicality of interactive e-modules was carried out through limited testing on 40 respondents who were PG PAUD students taking Science Learning courses for Early Childhood. A practicality questionnaire is the tool utilized to evaluate the benefits and convinience of use of this interactive e-module based on 21st century abilities. The practicality test"s outcmes demonstrate this 21st century skills-based interactive e-Module is very practical with a percentage of 94.1%. according to the respondents, this dynamic e-module was uncomplicated to use, fostered critical thinking skills, encouraged creativity, and gave them chances to interact and work together on early childhood science learning issues with their group members.