Practical high throughput screening

Reichman, Melvin; Harris, Andrea

doi:10.1007/978-0-306-46904-6_18

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…T HE FIRST PIN TOOLS introduced by robotics companies for HTS had a reputation of poor accuracy. [1][2][3] These early pin tools were made by a machining process that was difficult to reproduce. They were assembled by first testing each pin and then hand-sorting them into groupings that delivered similar volumes.…”

Section: Introductionmentioning

confidence: 99%

Nanoliter Dispensing for uHTS Using Pin Tools

Cleveland¹,

Koutz²

2005

ASSAY and Drug Development Technologies

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Miniaturization of assays is an important objective in Ultra-HTS. One of the major obstacles has been to find fluid handling systems capable of reliably and accurately delivering between 2 and 200 nl of test compound to assay plates. New methods of forming pins, placing slots in the pins, and hydrophobic coatings bonded onto the pins solve many of the problems encountered by early pin tools. Unlike other low-volume liquid handlers, these new pin tools provide the ability to transfer approximately 2 nl-5 microl of compounds. These pin tools can also use low-volume source plates (2-10 microl) and achieve an accuracy of better than 5%. This, coupled with the ability to transfer small volumes directly from the compound library to assay plates without an intervening dilution plate, saves reagents, throughput time, and consumables and is, therefore, very cost effective. Pin tool compound transfers in the 2-100 nl range provide a simple method to dilute away from the toxic effect that dimethyl sulfoxide has on some assay target cells. The factors that affect liquid transfers by pin tools are discussed in detail as well as the advantages and limitations of pin tools.

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Section: Introductionmentioning

confidence: 99%