PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ

Casado-Combreras, Miguel Á.; Rivero-Rodríguez, Francisco; Elena-Real, Carlos A.; Molodenskiy, Dmitry; Díaz‐Quintana, Antonio; Martinho, Marlène; Gerbaud, Guillaume; González‐Arzola, Katiuska; Velázquez‐Campoy, Adrián; Svergun, Dmitri I.; Belle, Valérie; Rosa, Miguel A. De la; Dı́az-Moreno, Irene

doi:10.1016/j.csbj.2022.07.009

Cited by 3 publications

(3 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The point mutation Q69C was inserted into the full‐length (SET/TAF‐Iβ‐Q69C) and the C‐terminal deletion (SET/TAF‐Iβ‐ΔC‐Q69C, amino acids 1–225) constructs as reported. [ 34 ] The C c ‐E104C mutant was produced as previously described. [ 35 ]…”

Section: Methodsmentioning

confidence: 99%

“…The point mutation Q69C was inserted into the full-length (SET/TAF-I𝛽-Q69C) and the C-terminal deletion (SET/TAF-I𝛽-ΔC-Q69C, amino acids 1-225) constructs as reported. [34] The Cc-E104C mutant was produced as previously described. [35] All proteins were expressed in Escherichia coli BL21(DE3) electrocompetent cells and grown in Luria-Bertani medium.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Histone Chaperones SET/TAF‐1β and NPM1 Exhibit Conserved Functionality in Nucleosome Remodeling and Histone Eviction in a Cytochrome c‐Dependent Manner

et al. 2023

View full text Add to dashboard Cite

Chromatin homeostasis mediates essential processes in eukaryotes, where histone chaperones have emerged as major regulatory factors during DNA replication, repair, and transcription. The dynamic nature of these processes, however, has severely impeded their characterization at the molecular level. Here, fluorescence optical tweezers are applied to follow histone chaperone dynamics in real time. The molecular action of SET/template‐activating factor‐Iβ and nucleophosmin 1—representing the two most common histone chaperone folds—are examined using both nucleosomes and isolated histones. It is shown that these chaperones present binding specificity for fully dismantled nucleosomes and are able to recognize and disrupt non‐native histone‐DNA interactions. Furthermore, the histone eviction process and its modulation by cytochrome c are scrutinized. This approach shows that despite the different structures of these chaperones, they present conserved modes of action mediating nucleosome remodeling.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Histone Chaperones SET/TAF‐1β and NPM1 Exhibit Conserved Functionality in Nucleosome Remodeling and Histone Eviction in a Cytochrome c‐Dependent Manner

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The γ-H2AX histone signals and recruits DDR factors on chromatin surrounding DSBs, so that γ-H2AX foci must be removed to proceed with the repair [ 288 ]. Using structural and computational approaches, it has been reported that Cyt- c forms a dynamic ensemble with SET/TAF-Iβ able to activate PP2A [ 289 ]. It is noteworthy that another histone chaperone and PP2A inhibitor—acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B)—is also targeted by the haemoprotein in the cell nucleus upon DSBs, yielding Cyt- c -mediated PP2A activation.…”

Section: From Crosstalk To Integral Signallingmentioning

confidence: 99%

Mitochondrial Factors in the Cell Nucleus

González-Arzola,

Díaz-Quintana

2023

IJMS

Self Cite

View full text Add to dashboard Cite

The origin of eukaryotic organisms involved the integration of mitochondria into the ancestor cell, with a massive gene transfer from the original proteobacterium to the host nucleus. Thus, mitochondrial performance relies on a mosaic of nuclear gene products from a variety of genomes. The concerted regulation of their synthesis is necessary for metabolic housekeeping and stress response. This governance involves crosstalk between mitochondrial, cytoplasmic, and nuclear factors. While anterograde and retrograde regulation preserve mitochondrial homeostasis, the mitochondria can modulate a wide set of nuclear genes in response to an extensive variety of conditions, whose response mechanisms often merge. In this review, we summarise how mitochondrial metabolites and proteins—encoded either in the nucleus or in the organelle—target the cell nucleus and exert different actions modulating gene expression and the chromatin state, or even causing DNA fragmentation in response to common stress conditions, such as hypoxia, oxidative stress, unfolded protein stress, and DNA damage.

show abstract

Cytochrome c prompts the recruitment of its nuclear partners SET/TAF-Iβ and NPM1 into biomolecular condensates

Casado-Combreras,

Velázquez-Campoy,

Martinho

et al. 2024

iScience

View full text Add to dashboard Cite

PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ

Cited by 3 publications

References 86 publications

The Histone Chaperones SET/TAF‐1β and NPM1 Exhibit Conserved Functionality in Nucleosome Remodeling and Histone Eviction in a Cytochrome c‐Dependent Manner

The Histone Chaperones SET/TAF‐1β and NPM1 Exhibit Conserved Functionality in Nucleosome Remodeling and Histone Eviction in a Cytochrome c‐Dependent Manner

Mitochondrial Factors in the Cell Nucleus

Cytochrome c prompts the recruitment of its nuclear partners SET/TAF-Iβ and NPM1 into biomolecular condensates

Contact Info

Product

Resources

About