About 200 to 300 million of sperms transverse from vagina to the cervix, only few hundreds of them can achieve the oocyte.The aim is theassessment ofpost coital test in females with unexplained infertility.In this study, 260 infertile women during 14-16-day of the cycle were considered. The women were subjected to the post-coital test at 2 hours after intercourse. The study was conducted in Baghdad during the period from January -April, 2018-2019.The study revealed that less than half of females were over 35 years and more than half were educated from urban areas and housewives while half of them had recurrent urinary tract infection, vaginitis, diabetes mellitus and less than that had positive family history, anxiety and hypertension. About interventions, 30% of females had cautery, D&C, IUI and less had IVF. 100% had taken clomiphene drug.The mean of BMI was 32.5±2.5. Ultrasound monitoring was done, there were normal findings with patent tubes except part of cases had picture of polycystic ovarian syndrome and ovarian failure. For post coital test, small percentage was with normal amount and consistency (score 3) and high percentage was with abnormal spinnbarkheit and ferning tests, (mostly score 0 & score1). 75% of cases had acidic vagina with dead non motile sperms while 25% with alkaline vagina with average of 40% progressive active sperms, 30%Moderate progressive Liner motility with highly significance (P value<0.05). For cellularity 70% was less than 6-8/HPF. For sperm activity only 30% were motile sperms. For sperm count (3 days' absence), 30% had mild oligospermia, 20% had moderate oligospermia and 10% had sever oligospermia. Most of females had normal hormonal assay but there was gross deficiency in the level of DHEA and D3 (average mean was 90±5µg/dl, 25±2 IU respectively) with small part of them had elevation in FSH and Testosterone hormones.For dietary pattern, most of females had dairy product, salt, tea with sugar daily, more than half of them had acidic food, fast food, dried food, gas drinking daily while part of them had intake of fruit weekly and vegetables monthly. Half of them were smoking cigarette weekly.High vaginal acidity, impairment of cervical mucous with deficiency of DHEA and D3 in the females and oligospermia in the males should be considered in case of unexplained infertility.