Potentiation of Catechin Gallate-Mediated Sensitization of
            <i>Staphylococcus aureus</i>
            to Oxacillin by Nongalloylated Catechins

Stapleton, Paul; Shah, Saroj; Hara, Yukihiko; Taylor, Peter W.

doi:10.1128/aac.50.2.752-755.2006

Cited by 38 publications

(42 citation statements)

References 14 publications

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“…In our previous report [16], K + loss in case of AMO was 25.7 ppm for ATCC 43300 strain, while for the clinical isolates the average K + loss was 25.79 ± 0.16 ppm. The highest leakage (26.6 ppm) of potassium was observed for IMP against ATCC 43300, while for clinical isolates it was 26.79 ± 0.14.…”

Section: Resultsmentioning

confidence: 86%

“…Therefore, these non-galloylated catechins were suggested to increase the galloylated catechin (EGCG and ECG) binding capacity to the bacterial cell membrane, thus suggesting their probable combined action. Moreover, non-galloylated catechins also increased oxacillin sensitivity against MRSA [16]. These studies suggest the complex interactions between flavonoids and bacterial cell.…”

Section: Resultsmentioning

confidence: 99%

“…The damage to the cytoplasm membrane was assessed by potassium leakage for all bacteria (ATCC 43300 strain and clinical isolates) in the case of L, Q [16] and Q + L ( p < 0.05) (Figure 7). …”

Section: Resultsmentioning

confidence: 99%

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Effects of Luteolin and Quercetin in Combination with Some Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus

Amin

Khurram

Khan

et al. 2016

IJMS

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The present study was designed to evaluate the effects of flavonoids luteolin (L) and quercetin + luteolin (Q + L) in combination with commonly used antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates and S. aureus (ATCC 43300). Minimum inhibitory concentrations (MICs) of L and Q + L, as well as the MICs of flavonoids in combination with antibiotics were determined and results showed an increased activity of flavonoids with antibiotics. The synergistic, additive, or antagonistic relationships between flavonoids (L and Q + L) and antibiotics were also evaluated, and additive and synergistic effects were observed for some antibiotic + flavonoid combinations. In addition, some combinations were also found to damage the bacterial cytoplasmic membrane, as assessed through potassium leakage assay. The effects of flavonoids and flavonoids + antibiotics on mecA gene mutations were also tested, and no functional variation was detected in the coding region.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Luteolin and Quercetin in Combination with Some Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus

Amin

Khurram

Khan

et al. 2016

IJMS

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“…This phenomenon did not occur when the concentration of ECg was increased, suggesting that the ILSMR effect of ECg could be potentiated by C. Previously, the cis form of non-galloylated catechins such as (−)-EC and (−)-EGC was reported to enhance the ILSMR effect of ECg [28], but excluding non-galloylated catechins of the trans form such as C. Herein, we firstly reported the ILSMR effects of C in combination with ECg on multiple classic β-lactam antibiotics against not only a standard MRSA strain but also clinical MRSA strains in vitro and in vivo. …”

Section: Resultsmentioning

confidence: 99%

The Combination of Catechin and Epicatechin Gallate from Fructus Crataegi Potentiates β-Lactam Antibiotics Against Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro and in Vivo

Qin

Xiao

Бин

et al. 2013

IJMS

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Fructus crataegi (hawthorn) is the common name of all plant species in the genus Crataegus of the Rosaceae family. In the present study, three monomers of (+)-catechin (C), (−)-epicatechin gallate (ECg) and (−)-epigallocatechin (EGC) were isolated from the hawthorn under the guide of antibacterial sensitization activity. The bioactivity of the composite fraction in enhancing the antibacterial effect of oxacillin against methicillin-resistant Staphylococcus aureus (MRSA) was greater than that of the individual monomer of the hawthorn extract in vitro. Two-fold dilution and checkerboard methods were used to analyze antibacterial activity and screen for the combination and proportion of monomers with the best bioactivity. The result showed that C (128 mg/L) combined with ECg (16 mg/L) had the greatest effect and the combination also reduced the bacterial load in blood of septic mice challenged with a sublethal dose of MRSA, increased daunomycin accumulation within MRSA and down-regulated the mRNA expression of norA, norC and abcA, three important efflux pumps of MRSA. In summary, C and ECg enhanced the antibacterial effect of β-lactam antibiotics against MRSA in vitro and in vivo, which might be related to the increased accumulation of antibiotics within MRSA via suppression of important efflux pumps’ gene expression.

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“…Galloyl flavan-3-ols such as (−)-epicatechin gallate reduce the MICs of ␤-lactam antibiotics against some strains of meticillin-resistant S. aureus (MRSA) more than 512-fold [97]. A recent development in this area is the finding that non-galloylated flavan-3-ols, which are themselves unable to sensitise strains of MRSA to ␤-lactams, can potentiate galloyl flavan-3-ol-mediated sensitisation [98].…”

Section: Naturally Occurring Flavonoidsmentioning

confidence: 99%

Recent advances in understanding the antibacterial properties of flavonoids

Cushnie

Lamb

2011

International Journal of Antimicrobial Agents

998

672

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Antibiotic resistance is a major global problem and there is a pressing need to develop new therapeutic agents. Flavonoids are a family of plant-derived compounds with potentially exploitable activities, including direct antibacterial activity, synergism with antibiotics, and suppression of bacterial virulence. In this review, recent advances towards understanding these properties are described. Information is presented on the ten most potently antibacterial flavonoids as well as the five most synergistic flavonoid-antibiotic combinations tested in the last 6 years (identified from PubMed and ScienceDirect). Top of these respective lists are panduratin A, with minimum inhibitory concentrations (MICs) of 0.06-2.0 μg/mL against Staphylococcus aureus, and epicatechin gallate, which reduces oxacillin MICs as much as 512-fold. Research seeking to improve such activity and understand structure-activity relationships is discussed. Proposed mechanisms of action are also discussed. In addition to direct and synergistic activities, flavonoids inhibit a number of bacterial virulence factors, including quorum-sensing signal receptors, enzymes and toxins. Evidence of these molecular effects at the cellular level include in vitro inhibition of biofilm formation, inhibition of bacterial attachment to host ligands, and neutralisation of toxicity towards cultured human cells. In vivo evidence of disruption of bacterial pathogenesis includes demonstrated efficacy against Helicobacter pylori infection and S. aureus α-toxin intoxication.

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Potentiation of Catechin Gallate-Mediated Sensitization of Staphylococcus aureus to Oxacillin by Nongalloylated Catechins

Cited by 38 publications

References 14 publications

Effects of Luteolin and Quercetin in Combination with Some Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus

Effects of Luteolin and Quercetin in Combination with Some Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus

The Combination of Catechin and Epicatechin Gallate from Fructus Crataegi Potentiates β-Lactam Antibiotics Against Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro and in Vivo

Recent advances in understanding the antibacterial properties of flavonoids

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