Potential of silk sericin based nanofibrous mats for wound dressing applications

Gilotra, Sween; Chouhan, Dimple; Bhardwaj, Nandana; Nandi, Samit Kumar

doi:10.1016/j.msec.2018.04.077

Cited by 108 publications

(57 citation statements)

References 39 publications

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“…The effect of TCs on wound healing was estimated by gross observation and histology. In order to find out the mainly cell type or cell types as the recipient cells downstream of TCs cell-cell communication in LPS induced skin wound, co-culture models of human keratinocyte (HaCaT), human dermal microvascular endothelial cell (HDMEC) [12] or murine fibroblast (L929) cell lines [13] with TCs were established. Cell proliferation, migration and apoptosis, and ROS and inflammatory factors were examined in HaCaTs, HDMECs, and L929 cell lines were detected to study the potential mechanisms involved in TCs protection in the skin wound healing process.…”

Section: Introductionmentioning

confidence: 99%

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

et al. 2020

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Background: Cell proliferation and death are key components of wound healing and tissue repair. Telocytes (TCs) represent a newly discovered cell type that can protect tissue from acute injury via cell-cell communication with adjacent cells. The aim of this study was to use a mouse model of skin wound healing and lipopolysaccharide (LPS)induced cell injury to evaluate the effects of TCs on skin wound healing in vivo and in vitro. Material/methods: Immunohistochemical staining was performed to evaluate the alteration of TCs in tissues from normal and chronic wound patients. Then, a male C57BL/6 mouse wound model of the back was established. The mice were divided randomly into three groups, and wound healing was estimated according to the wound healing rate and histology. An LPS-induced co-culture model of a mouse lung telocyte cell line (TCs) with human keratinocyte (HaCaT), human dermal microvascular endothelial cell (HDMEC) or murine fibroblast (L929) cell lines was established to analyse the effects of TCs on constitutive cell types of the skin. Cell proliferation, migration and apoptosis were examined, and reactive oxygen species (ROS) and inflammatory factors in HaCaT cells, HDMECs, and L929 cells were detected to study the mechanisms involved in TC protection in skin wounds. Results: TCs were significantly increased in tissues from chronic wound patients compared with healthy controls. Wound healing was significantly improved in wound mouse models treated with exogenous TCs compared with LPSinduced models. TCs reversed the LPS-induced inhibition of HaCaT cells and HDMECs and reduced the LPS-induced apoptosis of HaCaT cells and the death ratios of HDMECs and L929 cells. TCs reversed LPS-induced ROS in HDMECs and L929 cells and decreased inflammatory factor mRNA levels in HaCaT cells, HDMECs and L929 cells. Conclusions: TCs reduce wound healing delay, and inflammatory responses caused by LPS might be mediated by inflammatory inhibition, thus restricting apoptosis and promoting migration of the main component cell types in the skin.

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Section: Introductionmentioning

confidence: 99%

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

et al. 2020

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“…Due to the physicochemical properties described, SS is a potential biocompatible material and has many desirable properties for biomedical applications, such as resistance to oxidation and antibacterial, biodegradable, anticoagulation and anticancer activities . Wounds treated with SS‐containing materials have been shown to take less time to heal compared to non‐treated wounds .…”

Section: Introductionmentioning

confidence: 99%

Green fabrication route of robust, biodegradable silk sericin and poly(vinyl alcohol) nanofibrous scaffolds

Kumkun

Tuancharoensri

Ross

et al. 2019

Polymer International

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Silk sericin (SS) has been extensively used to fabricate scaffolds for tissue engineering. However, due to its inferior mechanical properties, it has been found to be a poor choice of material when being electrospun into nanofibrous scaffolds. Here, SS has been combined with poly(vinyl alcohol) (PVA) and electrospun to create scaffolds with enhanced physical properties. Crucially, these SS/PVA nanofibrous scaffolds were created using only distilled water as a solvent with no added crosslinker in an environmentally friendly process. Temperature has been shown to have a marked effect on the formation of the SS sol–gel transition and thus influence the final formation of fibers. Heating the spinning solutions to 70 °C delivered nanofibers with enhanced morphology, water stability and mechanical properties. This is due to the transition of SS from β‐sheets into random coils that enables enhanced molecular interactions between SS and PVA. The most applicable SS/PVA weight ratios for the formation of nanofibers with the desired properties were found to be 7.5/1.5 and 10.0/1.5. The fibers had diameters ranging from 60 to 500 nm, where higher PVA and SS concentrations promoted larger diameters. The crystallinity within the fibers could be controlled by manipulation of the balance between PVA and SS loadings. In vitro degradation (in phosphate buffer solution, pH 7.4 at 37 °C) was 30–50% within 42 days and fibers were shown to be nontoxic to skin fibroblast cells. This work demonstrates a new green route for incorporating SS into nanofibrous fabrics, with potential use in biomedical applications. © 2019 Society of Chemical Industry

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“…In small rodents, these “contaminated” sericin materials show pharmacological effects as diverse as improved stamina and increased intestinal absorption of metals ( 49 ). Tissue regenerative effects have also been suggested, and the potential of nanofibrous mats based on silk sericin/PVA electrospun material has been explored following subcutaneous implantation ( 50 ). Data in mice indicated good biocompatibility and low infection, and the authors suggested that the mulberry leave derived antioxidants within the material might impart superior properties to those of ‘inert’ dressings.…”

Section: Introductionmentioning

confidence: 99%

A Review of the Emerging Role of Silk for the Treatment of the Eye

2018

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Silk is a remarkable biopolymer with a long history of medical use. Silk fabrications have a robust track record for load-bearing applications, including surgical threads and meshes, which are clinically approved for use in humans. The progression of top-down and bottom-up engineering approaches using silk as the basis of a drug delivery or cell-loaded matrix helped to re-ignite interest in this ancient material. This review comprehensively summarises the current applications of silk for tissue engineering and drug delivery, with specific reference to the eye. Additionally, the review also covers emerging trends for the use of silk as a biologically active biopolymer for the treatment of eye disorders. The review concludes with future capabilities of silk to contribute to advanced, electronically-enhanced ocular drug delivery concepts.

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Potential of silk sericin based nanofibrous mats for wound dressing applications

Cited by 108 publications

References 39 publications

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

Green fabrication route of robust, biodegradable silk sericin and poly(vinyl alcohol) nanofibrous scaffolds

A Review of the Emerging Role of Silk for the Treatment of the Eye

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