Potential of Bacillus altitudinis KMS-6 as a biocontrol agent of Meloidogyne javanica

Antil, Sonam; Kumar, Rakesh; Pathak, D. V.; Panwar, Anil; Kumari, Anju; Kumar, Vinod

doi:10.1007/s10340-021-01469-x

Cited by 15 publications

(6 citation statements)

References 34 publications

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“…After that, the samples were cleaned with an air blower to remove the smaller chaff and lighter seeds, and a 15-cm-diameter chaff sieve was used to separate the larger debris and chaff. Finally, manual removal was used to remove any remaining immature or damaged seeds [23]. Afterward, the seeds were put in a paper bag and saved at 4°C.…”

Section: Weed Seed Collectionmentioning

confidence: 99%

Allelopathic Potential of Aqueous Plant Extracts Against Seed Germination and Seedling Growth of Weeds

Othman,

Lazim,

Al-Wakaa

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Allelopathy has been described as a negative or positive effect on a specific species of plant caused by a chemical produced by another species of plant. This research analyzed the allelopathic effect of various plant extracts against seed germination and seedling properties of some weed species in two experiments in the autumn of 2021. The impacts of plant extracts, such as peels of pomegranate (Punica granatum), green husk of walnuts (Juglans regia), leaves of eucalyptus (Eucalyptus camaldulensis), galls of Aleppo oak (Quercus infectoria), rhizomes of cogon grass (Imperata cylindrica), and tubers of nutgrass (Cyperus rotundus) plus control on some weed species, like Vaccaria pyramidata, Cynodon dactylon, Sorghum halepense, Trifolium campestre, Portulaca oleracea, Sinapis arvensis, Avena fatua), Hordeum glaucum and Daucus carota were evaluated. The first experiment was conducted in the lab and designed by a factorial-completely randomized design to investigate germination parameters such as final germination percentage (G), mean germination time (MGT), coefficient of the velocity of germination (CVG), and germination rate index (GRI), and another experiment was in a greenhouse and designed in a split-plot pattern, in which the plant extracts were placed in main plots. At the same time, the weed species were distributed in the sub-plots to evaluate the seedling of weeds’ fresh and dry weight and percent of weed inhibition (PWI). The characters’ means (from three replicates) were compared according to Duncan’s multiple range test at the 5% level. The laboratory trial revealed that the green walnut husk was the most successful in reducing all seed germination parameters compared to the control by 13.5, 5.16, and 8.37% for G, MGT, and GRI, respectively. For the greenhouse experiment, the eucalyptus leaves’ extract and pomegranate peels had a higher impact on reducing weed seedlings’ fresh and dry weight and PWI than the control. The most affected species were broad-leaved weeds, while grasses were the least affected. Our results indicated that green walnut husk has a significant role in decreasing seed germination and inhibiting the dry weight of seedlings. Thus, it can be used as a bioherbicide to prevent some weeds from sprouting and growing seedlings.

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Section: Weed Seed Collectionmentioning

confidence: 99%

Allelopathic Potential of Aqueous Plant Extracts Against Seed Germination and Seedling Growth of Weeds

Othman,

Lazim,

Al-Wakaa

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…These biocontrol bacteria’s metabolites will be a significant source of antibiotic compounds to prevent Verticillium wilt. B. altitudinis , in particular, is considered probiotics that have no harmful effects on the environment or the human being [ 27 ]. As a result, B. altitudinis , which can effectively antagonize V. dahliae , is a potential biocontrol strain for controlling Verticillium wilt in cotton.…”

Section: Introductionmentioning

confidence: 99%

Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

Shan,

Wang,

Zhao

et al. 2024

BMC Biol

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Background Verticillium wilt, caused by the fungus Verticillium dahliae, is a soil-borne vascular fungal disease, which has caused great losses to cotton yield and quality worldwide. The strain KRS010 was isolated from the seed of Verticillium wilt-resistant Gossypium hirsutum cultivar “Zhongzhimian No. 2.” Results The strain KRS010 has a broad-spectrum antifungal activity to various pathogenic fungi as Verticillium dahliae, Botrytis cinerea, Fusarium spp., Colletotrichum spp., and Magnaporthe oryzae, of which the inhibition rate of V. dahliae mycelial growth was 73.97% and 84.39% respectively through confrontation test and volatile organic compounds (VOCs) treatments. The strain was identified as Bacillus altitudinis by phylogenetic analysis based on complete genome sequences, and the strain physio-biochemical characteristics were detected, including growth-promoting ability and active enzymes. Moreover, the control efficiency of KRS010 against Verticillium wilt of cotton was 93.59%. After treatment with KRS010 culture, the biomass of V. dahliae was reduced. The biomass of V. dahliae in the control group (Vd991 alone) was 30.76-folds higher than that in the treatment group (KRS010+Vd991). From a molecular biological aspect, KRS010 could trigger plant immunity by inducing systemic resistance (ISR) activated by salicylic acid (SA) and jasmonic acid (JA) signaling pathways. Its extracellular metabolites and VOCs inhibited the melanin biosynthesis of V. dahliae. In addition, KRS010 had been characterized as the ability to promote plant growth. Conclusions This study indicated that B. altitudinis KRS010 is a beneficial microbe with a potential for controlling Verticillium wilt of cotton, as well as promoting plant growth.

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“…During the life cycle of RKN, eggs and second-stage juveniles (J2) are exposed outside the roots, making them the targets of most nematicides. The application of chemical nematicides remains the predominant approach to effectively managing RKN ( Antil et al, 2022 ). Still, their efficacies decrease with prolonged application, increasing environmental and human health risks and causing nematode multidrug resistance ( Rajasekharan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring the nematicidal mechanisms and control efficiencies of oxalic acid producing Aspergillus tubingensis WF01 against root-knot nematodes

Yang,

Dai,

et al. 2024

Front. Microbiol.

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Background and aimsRoot-knot nematodes (RKN; Meloidogyne spp.) are among the highly prevalent and significantly detrimental pathogens that cause severe economic and yield losses in crops. Currently, control of RKN primarily relies on the application of chemical nematicides but it has environmental and public health concerns, which open new doors for alternative methods in the form of biological control.MethodsIn this study, we investigated the nematicidal and attractive activities of an endophytic strain WF01 against Meloidogyne incognita in concentration-dependent experiments. The active nematicidal metabolite was extracted in the WF01 crude extract through the Sephadex column, and its structure was identified by nuclear magnetic resonance and mass spectrometry data.ResultsThe strain WF01 was identified as Aspergillus tubingensis based on morphological and molecular characteristics. The nematicidal and attractive metabolite of A. tubingensis WF01 was identified as oxalic acid (OA), which showed solid nematicidal activity against M. incognita, having LC50 of 27.48 μg ml–1. The Nsy-1 of AWC and Odr-7 of AWA were the primary neuron genes for Caenorhabditis elegans to detect OA. Under greenhouse, WF01 broth and 200 μg ml–1 OA could effectively suppress the disease caused by M. incognita on tomatoes respectively with control efficiency (CE) of 62.5% and 70.83%, and promote plant growth. In the field, WF01-WP and 8% OA-WP formulations showed moderate CEs of 51.25%–61.47% against RKN in tomato and tobacco. The combined application of WF01 and OA resulted in excellent CEs of 66.83% and 69.34% toward RKN in tomato and tobacco, respectively. Furthermore, the application of WF01 broth or OA significantly suppressed the infection of J2s in tomatoes by upregulating the expression levels of the genes (PAL, C4H, HCT, and F5H) related to lignin synthesis, and strengthened root lignification.ConclusionAltogether, our results demonstrated that A. tubingensis WF01 exhibited multiple weapons to control RKN mediated by producing OA to lure and kill RKN in a concentration-dependent manner and strengthen root lignification. This fungus could serve as an environmental bio-nematicide for managing the diseases caused by RKN.

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Potential of Bacillus altitudinis KMS-6 as a biocontrol agent of Meloidogyne javanica

Cited by 15 publications

References 34 publications

Allelopathic Potential of Aqueous Plant Extracts Against Seed Germination and Seedling Growth of Weeds

Allelopathic Potential of Aqueous Plant Extracts Against Seed Germination and Seedling Growth of Weeds

Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae

Exploring the nematicidal mechanisms and control efficiencies of oxalic acid producing Aspergillus tubingensis WF01 against root-knot nematodes

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