Potential of a new strain of Bacillus amyloliquefaciens BUZ-14 as a biocontrol agent of postharvest fruit diseases

Calvo, Héctor; Marco, Pedro; Blanco, Domingo; Oria, Rosa; Venturini, Marı́a Eugenia

doi:10.1016/j.fm.2016.11.004

Cited by 121 publications

(77 citation statements)

References 66 publications

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“…The decay caused by microbial invasion results in major losses of postharvest fruits and vegetables, which creates a huge economic burden. It has been estimated that about 20%–25% of harvested fruits and vegetables decay from filamentous fungal infections during postharvest handling, even in developed countries; therefore, the control of such infections is critical (Calvo, Marco, Blanco, Oria, & Venturini, ). Gray mold, caused by Botrytis cinerea , is one of the most destructive diseases in grape production (Fu, Qu, Yang, & Zhang, ; Jaspers, Seyb, Trought, & Balasubramaniam, ; Liu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes

Zhou

et al. 2020

Food Science & Nutrition

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We investigated the effects and possible mechanisms of Bacillus amyloliquefaciens NCPSJ7 against the gray mold caused by Botrytis cinerea in the postharvest Red Globe grapes. The disease incidence, lesion diameter, decay index, and some resistance‐related enzymes were evaluated. The antioxidant capacity of grape treated with 1 × 104 CFU/ml B. cinerea alone and combined with 1 × 107 CFU/ml NCPSJ7 was also determined. The results showed that NCPSJ7 + B. cinerea reduced the disease incidence, lesion diameter, and decay index of postharvest grapes and enhanced the activities of polyphenol oxidase, peroxidase, chitinase, and β‐1,3‐glucanase during different storage periods. Furthermore, the oxidative resistance, demonstrated by an escalating trend in the total phenolic content, DPPH free radical clearance rate, reducing power, and superoxide anion clearance rate after lesion presence, was improved. However, NCPSJ7 showed an inhibitory effect on gray mold, but resulted in the reduced antioxidant capacity in the grapes.

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Section: Introductionmentioning

confidence: 99%

Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes

Zhou

et al. 2020

Food Science & Nutrition

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“…However, the mycosubtilin gene was neither found in BUZ-14 (Supplemental Table 1) nor later detected by HPLC, so this option was discarded. Arrebola et al (2010) found that iturin A produced by B. amyloliquefaciens PPCB004 appeared at a Rf of 0.3 but Yánez-Mendizábal et al (2012) obtained the same Rf for bacillomycin D. These findings, added to the fact that FZB42 produces another lipopeptide with a Rf of 0.3 (bacillomycin), make it impossible to elucidate by TLC whether BUZ-14 was an iturin A or a bacillomycin D producer. Moreover, FZB42 did not show inhibition in the bioautography against any pathogen, indicating that both metabolites developed in the TLC at the same Rf could be different.…”

Section: Isolation and Identification Of Lipopeptides From B Amylolimentioning

confidence: 99%

“…The chemical structure has previously been studied by numerous authors, regarding the type and sequence of amino acid residues, the nature of the peptide and length and branching of the fatty acid chain (Hinarejos et al, 2016;Ongena and Jacques, 2008;Stein, 2005;Torres et al, 2016;Xu et al, 2013). The mechanism of direct antibiosis associated with Bacillus strains against foliar and postharvest diseases has been attributed mostly to M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 4 iturins and fengycins (Arrebola et al, 2010;Romero et al, 2007;Yánez-Mendizábal et al, 2012) although other metabolites such as polyketides (Chen et al, 2006;, siderophores (Li et al, 2014), bacteriocins (Ayed et al, 2015 and volatile organic compounds (VOCs) (Gotor-Vila et al, 2017) must not be forgotten. These metabolites and compounds can be produced by the biological control agent (BCA) in the formulation prior to the application with the mechanism of action based on a direct antibiosis.…”

Section: Introductionmentioning

confidence: 99%

The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots

et al. 2019

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The aim of this work was to elucidate the role of the secondary metabolites produced by B. amyloliquefaciens BUZ-14 against B. cinerea, M. fructicola, M. laxa, P. digitatum, P. italicum and P. expansum both in vitro and in planta. The entire cell free supernatant (CFS) and the lipopeptide fraction (LPF) showed similar antifungal activities, completely inhibiting all the fungi at dilutions of 1:24 or even lower, whereas the nonbutanolic fraction (NBF) barely inhibited the fungi. However, when the LPF and CFS were applied on fruit, only brown rot in peaches and blue rot in apples was totally inhibited. The main families of metabolites in the LPF were iturin A, fengycin and surfactin with maximum concentrations of 407, 853 and 658 µg mL-1 , respectively. Subsequently, a TLC-bioautography revealed iturin A as the key metabolite in the inhibitions and allowed us to establish in vivo MICs of 16.9 and 33.9 µg mL-1 for Monilinia species and P. expansum, respectively. The application of 24 hold BUZ-14 cultures supressed brown rot in peaches and also blue rot in apples but failed to inhibit the other diseases. However, BUZ-14 was only able to grow and produce iturin A in peaches so we can deduce that the amount of iturin A brought with the cultures (36 ± 14 µg mL-1) could be enough to control both diseases. The strong antifungal activity of the iturin A present in the BUZ-14 CFS suggests that it could be successfully used for postharvest disease control. However, future research is necessary to maximize the iturin A production by B. amyloliquefaciens BUZ-14 in order to optimize a commercial application.

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“…Bacillus species are widespread and are mainly found in the plant root system. They are very heat tolerant; grow quickly in fluid cultures, and easily produce resistant spores (Calvo, Marco, Blanco, Oria, & Venturini, 2017). Bacillus amyloliquefaciens represents one of the promising strains belonging to Bacillus genera, which successfully limited the proliferation of the PAT‐producing molds on fruits.…”

Section: Current Application Of Biotechnology In the Face Of The Foodmentioning

confidence: 99%

Recent trends in detecting, controlling, and detoxifying of patulin mycotoxin using biotechnology methods

Ngea

Yang

Castoria

et al. 2020

Comp Rev Food Sci Food Safe

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Patulin (PAT) is a mycotoxin that can contaminate many foods and especially fruits and fruit‐based products. Therefore, accurate and effective testing is necessary to enable producers to comply with regulations and promote food safety. Traditional approaches involving the use of chemical compounds or physical treatments in food have provided practical methods that have been used to date. However, growing concerns about environmental and health problems associated with these approaches call for new alternatives. In contrast, recent advances in biotechnology have revolutionized the understanding of living organisms and brought more effective biological tools. This review, therefore, focuses on the study of biotechnology approaches for the detection, control, and mitigation of PAT in food. Future aspects of biotechnology development to overcome the food safety problem posed by PAT were also examined. We find that biotechnology advances offer novel, more effective, and environmental friendly approaches for the control and elimination of PAT in food compared to traditional methods. Biosensors represent the future of PAT detection and use biological tools such as aptamer, enzyme, and antibody. PAT prevention strategies include microbial biocontrol, the use of antifungal biomolecules, and the use of microorganisms in combination with antifungal molecules. PAT detoxification aims at the breakdown and removal of PAT in food by using enzymes, microorganisms, and various adsorbent biopolymers. Finally, biotechnology advances will be dependent on the understanding of fundamental biology of living organisms regarding PAT synthesis and resistance mechanisms.

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Potential of a new strain of Bacillus amyloliquefaciens BUZ-14 as a biocontrol agent of postharvest fruit diseases

Cited by 121 publications

References 66 publications

Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes

Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes

The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots

Recent trends in detecting, controlling, and detoxifying of patulin mycotoxin using biotechnology methods

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