Potential biomarkers of infertility associated with microbiome imbalances

Azpiroz, M.A; Orguilia, Lucila; Palacio, Maria Ines; Malpartida, Alejandro; Mayol, Soledad; Gil, Mor; Gutiérrez, Gabriela

doi:10.1111/aji.13438

Cited by 16 publications

(10 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…15 Studies have explored the possible relationship between vaginal and rectal microbiome disbalances with infertility. 16 The 16S sequencing of vaginal, intestinal, urethral and oral secretions of the same participant showed significant differences in bacterial populations in these secretions.Studies have shown that the microbial composition in the upper genital tract, uterine cavity, fallopian tube, ovary, and other parts of the same participant is not completely identical. 17 Vaginal flora is related to female fertility, but its effects on the clinical pregnancy rate of AIH have not been reported.…”

Section: Discussionmentioning

confidence: 93%

“…It is estimated that the total number of bacteria within the human microbiota is the same order of magnitude as the total number of cells that make up the human body 15 . Studies have explored the possible relationship between vaginal and rectal microbiome disbalances with infertility 16 . The 16S sequencing of vaginal, intestinal, urethral and oral secretions of the same participant showed significant differences in bacterial populations in these secretions.Studies have shown that the microbial composition in the upper genital tract, uterine cavity, fallopian tube, ovary, and other parts of the same participant is not completely identical 17 …”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Associations between vaginal flora, MIP‐1α, IL‐17A, and clinical pregnancy rate in AIH

Yang

Gao

Wang

et al. 2022

American J Rep Immunol

View full text Add to dashboard Cite

Problem To investigate how asymptomatic bacterial imbalance affects the clinical pregnancy rate after artificial insemination with the husband's semen (AIH). Methods This study included married heterosexual couples who underwent AIH. According to the follow‐up results, participants were divided into the pregnancy and non‐pregnancy groups. Based on the first 10 pair participants in each group with vaginal flora bacterial 16S rRNA sequencing results, six semen samples received bacterial‐sperm mixed test. Moreover, 34 cytokines were detected in the peripheral blood sera of the first three pairs by high‐throughput Luminex, which were verified in vaginal secretions, cervical mucus, and blood sera from the first 200 pairs by ELISA. Results The results of the 16S sequencing of vaginal secretions showed that compared with the pregnant group, the non‐pregnant group had a significantly increased bacterial species diversity, which was mainly manifested by a decrease in Lactobacillus crispatus and an increase in Prevotella bivia. When Prevotella bivia or Lactobacillus crispatus were mixed with sperms, the sperm motility was decreased (p < .05). The vaginal posterior fornix secretions, cervical mucus, and peripheral blood sera of the non‐pregnant group showed decreased levels of MIP‐1α and increased levels of IL‐17A (p < .05). Conclusion The imbalance of vaginal flora leading to the increase of Prevotella bivia and the decrease of Lactobacillus crispatus may cause an imbalance of immune regulation. Low expression of MIP‐1α and high expression of IL‐17A were associated with reduced clinical pregnancy rate in AIH.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Associations between vaginal flora, MIP‐1α, IL‐17A, and clinical pregnancy rate in AIH

Yang

Gao

Wang

et al. 2022

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

“…Last, the gut microbiota is implicated in nutrient absorption, and de ciencies in trace elements and vitamins could have repercussions on fertility[66-68]. Concurrently, the gut microbiota's impact on body weight and metabolism should not be overlooked, as weight anomalies-be they obesity or underweight-are established to be detrimental to fertility [49,[69][70][71].…”

Section: Discussionmentioning

confidence: 99%

Causal Effects of Gut Microbiota on Female Reproductive Tract Inflammation and Infertility: A Mendelian Randomization Study

Feng,

Chen,

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Studies indicate a potential connection between gut microbiota and reproductive health issues in women, such as inflammation and infertility. However, the exact relationship remains uncertain, highlighting the need for further research. Results A two-sample Mendelian Randomization (MR) analysis was conducted to investigate the relationship between gut microbiota and pelvic inflammatory diseases and infertility. The study identified that specific gut microbiota, such as Lachnospiraceae UCG001 and Ruminococcus 2, increased the risk of inflammatory disorders in female pelvic organs (OR [95%CI]: 1.13 [1.01–1.26] and 1.16 [1.03–13.1], respectively). In contrast, Butyricicoccus and Prevotella 7 were associated with a reduced risk. Further validation with another dataset confirmed Butyricicoccus's role in decreasing pelvic inflammatory disease risk. The study also found a causal relationship between gut microbiota and inflammation in specific pelvic organs, including salpingitis and oophoritis, uterine inflammation, cervical inflammation, and vulvar or vaginal inflammation. Regarding female infertility, Faecalibacterium was linked to an increased risk (1.31 [1.08–1.60]), while genera like Erysipelotrichaceae UCG003 and Ruminococcus (torques group) were associated with lower risks. Further analyses showed a causal connection between gut microbiota and various types of infertility, including anovulation, tubal factors, and others. These results were further confirmed by sensitivity analysis, and no reverse causation was found. Conclusions This study provides compelling evidence for the causal relationship between gut microbiota and female reproductive health. Identification of specific bacterial genera associated with increased or decreased risk offers new possibilities for early diagnosis and targeted treatment.

show abstract

“…DNA and RNA were extracted simultaneously following the mirVana miRNA isolation kit for RNA protocol (Ambion, ThermoFisher Scientific, Waltham, MA). We chose this isolation kit as it is designed to efficiently isolate small RNAs (<200 nt) as well as total RNA which play a crucial role in regulating bacterial global gene expression and is routinely used for microbiome studies (Azpiroz et al 2021; Tong, Tang, and Wang 2023; Yost et al 2018). For RNA isolation, genomic DNA was removed using a Turbo DNA-free kit (Applied Biosystems, Ambion).…”

Section: Methodsmentioning

confidence: 99%

Heterogeneous lineage-specific arginine deiminase expression within dental microbiome species

Mann,

Chakraborty,

O’Connell

et al. 2023

Preprint

View full text Add to dashboard Cite

Arginine catabolism by the bacterial arginine deiminase system (ADS) has anticariogenic properties through the production of ammonia, which modulates the pH of the oral environment. Given the potential protective capacity of the ADS pathway, the exploitation of ADS competent oral microbes through pre- or probiotic applications is a promising therapeutic target to prevent tooth decay. To date, most investigations of the ADS in the oral cavity and its relation to caries have focused on indirect measures of activity, or on specific bacterial groups, yet the pervasiveness and rate of expression of the ADS operon in diverse mixed microbial communities in oral health and disease remains an open question. Here we use a multivariate approach, combining ultra-deep metatranscriptomic sequencing with paired metataxonomic andin vitrocitrulline quantification to characterize the microbial community and ADS operon expression in healthy and late-stage cavitated teeth. While ADS activity is higher in healthy teeth, we identify multiple bacterial lineages with upregulated ADS activity on cavitated teeth that are distinct from those found on healthy teeth using both reference-based mapping anddenovoassembly methods. Our dual metataxonomic and metatranscriptomic approach demonstrates the importance of species abundance for gene expression data interpretation and that patterns of differential expression can be skewed by low abundance groups. Finally, we identify several potential candidate probiotic bacterial lineages within species that may be useful therapeutic targets for the prevention of tooth decay and propose that the development of a strain-specific, mixed-microbial probiotic may be a beneficial approach given the heterogeneity of taxa identified here across health groups.IMPORTANCETooth decay is the most common preventable chronic disease, globally affecting more than two billion people. The development of caries on teeth is primarily a consequence of acid production by cariogenic bacteria that inhabit the plaque microbiome. Other bacterial strains in the oral cavity may suppress or prevent tooth decay by producing ammonia as a byproduct of the arginine deiminase metabolic pathway, increasing the pH of the plaque biofilm. While the benefits of arginine metabolism on oral health have been extensively documented in specific bacterial groups, the prevalence and consistency of ADS activity among oral bacteria in a community context remains an open question. In the current study, we use a multi-omics approach to document the pervasiveness of expression of the ADS operon in both health and disease to better understand the conditions in which ADS activity may prevent tooth decay.

show abstract

Potential biomarkers of infertility associated with microbiome imbalances

Cited by 16 publications

References 77 publications

Associations between vaginal flora, MIP‐1α, IL‐17A, and clinical pregnancy rate in AIH

Associations between vaginal flora, MIP‐1α, IL‐17A, and clinical pregnancy rate in AIH

Causal Effects of Gut Microbiota on Female Reproductive Tract Inflammation and Infertility: A Mendelian Randomization Study

Heterogeneous lineage-specific arginine deiminase expression within dental microbiome species

Contact Info

Product

Resources

About