1995
DOI: 10.1021/bi00036a009 View full text |Buy / Rent full text
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Abstract: Screening of random oligonucleotide libraries with SELEX [systematic evolution of ligands by exponential enrichment; Tuerk, C., & Gold, L. (1990) Science 249, 505-510] has emerged as a powerful method for identifying high-affinity nucleic acid ligands for a wide range of molecular targets. Nuclease sensitivity of unmodified RNA and DNA, however, imposes considerable restrictions on their use as therapeutics or diagnostics. Modified RNA in which pyrimidine 2'-hydroxy groups have been substituted with 2'-amino g… Show more

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“…Being smaller than antibodies, aptamers are better candidates for cell penetration and blood clearance. A variety of chemical modifications, such as fluorescent probes, cross-linking reagents, and modifications to the backbone or specific bases by fluorine (2Ј-hydroxyl groups of the ribose moieties are replaced with fluorine) (7), can be introduced, thereby adding stability and functionality. Moreover, aptamers are nonimmunogenic and therefore do not cause side effects resulting from unwanted immune responses in hosts.…”
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rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…Being smaller than antibodies, aptamers are better candidates for cell penetration and blood clearance. A variety of chemical modifications, such as fluorescent probes, cross-linking reagents, and modifications to the backbone or specific bases by fluorine (2Ј-hydroxyl groups of the ribose moieties are replaced with fluorine) (7), can be introduced, thereby adding stability and functionality. Moreover, aptamers are nonimmunogenic and therefore do not cause side effects resulting from unwanted immune responses in hosts.…”
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“…Selectionreflection thus joins the approach of using modified RNA polymerase substrates (11)(12)(13)(14) as a viable means of coupling the power of in vitro selection to the production of stable bioactive ligands.…”
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“…Селекция РНК к FIХa была проведена Rusconi C.P. с сотрудниками, и после восьми раундов селекции был найден аптамер, который связывался с FIХa с К d = 0,65±0,2 пМ и обладал 5000-кратным сродством к FIХa по сравнению с факторами FVIIa, FХa, FХIa и активированным протеином С [20]. Укороченная версия аптамера к FIХa (9.3t) сохраняла высокое сродство к FIХa (К d = 0,58±0,1 пМ) и полностью блокировала гидролиз FХ энзимным комплексом.…”
Section: фактор Iхaunclassified
“…С помощью метода SELEX были выполнены селекционные циклы против VEGF 165 [19,20,[53][54][55] с использованием 2`-фторзамещенных пиримидинов в РНК библиотеке, Ruckman J. провел 12 раундов селекции и выделил аптамеры с К d = 50 пМ. Для увеличения стабильности против нуклеаз два аптамера дополнительно модифицировали по 2`-О-положению [55].…”
Section: Vegfunclassified
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