Potency of Lobak Leaves (Raphanus sativus L. var. hortensis Back) as Anticancer and Antimicrobial Candidates

Rakhmawati, Rita; Anggarwulan, Endang; Retnaningtyas, Estu

doi:10.13057/biodiv/d100310

Cited by 12 publications

(13 citation statements)

References 12 publications

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“…This indicated that the toxic compounds in the EC exerted a synergistic effect [35]. Based on the anticancer preliminary test (toxicity test), the EC was the most toxic extract, indicating that the toxic compounds are semipolar because they dissolve into chloroform (Table-2) [36]. As shown in Figure-1, the sample concentration was positively correlated with the inhibition of HeLa cell growth.…”

Section: Discussionmentioning

confidence: 99%

Anticancer activities of toxic isolate of Xestospongia testudinaria sponge

et al. 2019

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Aims:The purposes of this study were to determine the anticancer activity of Xestospongia testudinaria sponge isolate and identify the responsible compounds.Materials and Methods:The metabolites were extracted using methanol maceration at room temperature. The separation and purification of metabolites were performed using fractionation and column chromatography. The toxicity was examined using the brine shrimp lethality assay, and the toxic isolates were tested for anticancer activity against HeLa cells. Gas chromatography-mass spectrometry analysis was used to identify the compounds in the isolate.Results:When the methanol extract was partitioned with n-hexane, chloroform, and n-butanol, the chloroform fraction was the most toxic, with a concentration that results in 50% lethality (LC50) value of 39.81 ppm. After separation of the chloroform extract, fraction B (FB) was the most toxic, with an LC50 value of 44.67 ppm. The isolate from FB showed anticancer activity with a concentration at which 50% of growth was inhibited (IC50) of 2.273 ppm. In total, 21 compounds were identified in anticancer isolates: Nonanedioic acid; tetradecanoic acid; trans-phytol; 2-pentadecanone-6,10,14-trimethyl; pentadecanoic acid; 2-hexadecen-1-ol, 3,7,11,15-tetramethyl-; pentadecanoic acid; 2-hexadecen-1-ol, 3,7,11,15-tetramethyl-; 2,3,7-trimethyloctanal; palmitic acid; docosanoic acid, ethyl ester; 1,E-11,Z-13-octadecatriene; chloromethyl 4-chlorododecanoate; 1-tricosene; 9,12-octadecadienoic acid; 4,8,12,16-tetramethylheptadecan-4-olide; 1-docosene; heneicosane; phosphonic acid, dioctadecyl ester; dodecane,4,6-dimethyl-; n-tetratriacontane; 1-iodohexadecane; and n-heneicosane.Conclusion:These findings indicate that the isolate of X. testudinaria can be used as a natural anticancer toward HeLa cell.

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Section: Discussionmentioning

confidence: 99%

Anticancer activities of toxic isolate of Xestospongia testudinaria sponge

et al. 2019

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“…Ethyl acetate fraction from methanol extract is most active that had larger clear zone in S. aureus culture (10,64 mm). Bioactive compounds at active fraction were identified to contain polar compounds [24] .…”

Section: Anti Inflammatory Activitymentioning

confidence: 99%

A Disquisition on Raphanus sativus Linn- A Propitious Medicinal Plant

Sabishruthi¹,

Rajan²,

Sai³

et al. 2018

IJCTR

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Although with the invention of hundreds of allopathic drug preparations and its various combinations, yet the belief on traditional medications for the curing of ailments has not come down. One such medication among the many others is Raphanus sativus. The entire plant part has medicinal benefits starting from the roots to the leaves. The extracts of roots are used for treatment of urinary complaints, haemorrhoids, syphilitic disease; reputed medicine for piles etc., the extract obtained from seeds has been used as expectorant, digestive, diuretic, laxative, stomach tonic, carminative, and antitussive. It has anticancer, antimicrobial, antidiabetic, antiurolithiatic, antifertility, hypertensive, nephroprotective, gastroprotective and hepatoprotective properties, in addition used for gynaecological conditions and jaundice. The chemical constituents present are alkaloids, nitrogen compounds, coumarins, enzymes, gibberellins, glucosinolates, oil seed compounds, organic acids, phenolic compounds, sulphur compounds, flavonoids, saponins, carbohydrates, proteins, amino acids, tannins, antraquinones, steroids, terpenoids, cardiac glycosides, chalcones, brassinosteroids and polyphenols. The seeds and leaves of a plant contain "raphanin" which contains isothiocyanates and dithiolthiones to help lower the risk of cancer. The present study is thus on a review of Raphanus sativus which is a highly potential, functional and valuable ingredient that exhibits relatively impressive biological functions of great interest in pharmaceutical and food industries.

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“…Melalui metode BSLT pelaksanaan skrining awal dapat dilakukan dengan biaya yang relatif mudah, sederhana, dan relatif cepat terhadap standarisasi bioaktifitas pada produksi tumbuhan heterogen (12). Suatu ekstrak dikatakan bersifat toksik jika memiliki nilai LC50 (konsentrasi yang mampu membunuh 50% larva udang) kurang dari 1000 ppm setelah waktu kontak 24 jam (13).…”

Section: Perhitungan Nilai Lc50unclassified

Skrining antikanker menggunakan metode BST (Brine Shrimp Lethality Test) pada ekstrak metanol daun saga (Abrus precatorius L.) dengan partisi etanol

Dhanti

2018

RIK

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Latar belakang : Pengembangan senyawa antikanker dari bahan alami perlu dilakukan untuk meminimalisir efek samping dari penanganan penyakit kanker yang saat ini banyak dilakukan. Suatu senyawa dapat diketahui potensi antikankernya dengan pendekatan menggunakan metode BST (Brine Shrimp Lethality Test). Ekstrak metanol daun saga (Abrus precatorius L.) bersifat toksik terhadap larva A. salina Leach. Tujuan : Mengetahui bagian teraktif dari ekstrak metanol daun saga yang dipartisi (dipisahkan) menggunakan pelarut etanol. Metode : Ekstrak metanol daun saga dipartisi dengan pelarut etanol hingga terbentuk bagian larut dan tidak larut. Kedua bagian tersebut diuji menggunakan metode BST dengan 5 kali ulangan dan 3 replikasi yang masing-masing menggunakan 10 ekor larva A.salina. Hasil : Dari perhitungan didapatkan nilai LC50 bagian larut etanol sebesar 144,544 ppm sedangkan nilai LC50 bagian tidak larut etanol sebesar 151,356 ppm. Kesimpulan : Bagian larut etanol menyebabkan persentase kematian yang lebih tinggi daripada bagian tidak larut. Nilai LC50 bagian larut etanol lebih rendah dibanding bagian tidak larutnya. Semakin rendah nilai LC50 senyawa, maka semakin berpotensi pula untuk dikembangkan sebagai agen antikanker. Kata kunci : toksisitas, Abrus precatorius L., Artemia salina Leach., antikanker, partisi.

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Potency of Lobak Leaves (Raphanus sativus L. var. hortensis Back) as Anticancer and Antimicrobial Candidates

Cited by 12 publications

References 12 publications

Anticancer activities of toxic isolate of Xestospongia testudinaria sponge

Anticancer activities of toxic isolate of Xestospongia testudinaria sponge

A Disquisition on Raphanus sativus Linn- A Propitious Medicinal Plant

Skrining antikanker menggunakan metode BST (Brine Shrimp Lethality Test) pada ekstrak metanol daun saga (Abrus precatorius L.) dengan partisi etanol

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