Potencial herramienta en la personalización del tratamiento oncológico: Casos clínicos

C, Carolina Ibáñez; S, Marcelo Garrido; Medina, Lidia; Kato, Sumie; Bravo, María Loreto; González, Pamela; Oliva, Bárbara; Pizarro, Javier; Bustamante, Eva; Brañes, Jorge; Cuello, Mauricio; Owen, Gareth I.

doi:10.4067/s0034-98872013000500017

Cited by 2 publications

(1 citation statement)

References 12 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cancer type and stage are included in Table 1 . Human cancer cells were isolated from ovarian cancer ascites as previously reported [ 27 - 29 ]. Primary cultured cells in passage 2 were typically used for all experimentation.…”

Section: Methodsmentioning

confidence: 99%

Platelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundAn increase in circulating platelets, or thrombocytosis, is recognized as an independent risk factor of bad prognosis and metastasis in patients with ovarian cancer; however the complex role of platelets in tumor progression has not been fully elucidated. Platelet activation has been associated with an epithelial to mesenchymal transition (EMT), while Tissue Factor (TF) protein expression by cancer cells has been shown to correlate with hypercoagulable state and metastasis. The aim of this work was to determine the effect of platelet-cancer cell interaction on TF and “Metastasis Initiating Cell (MIC)” marker levels and migration in ovarian cancer cell lines and cancer cells isolated from the ascetic fluid of ovarian cancer patients.MethodsWith informed patient consent, ascitic fluid isolated ovarian cancer cells, cell lines and ovarian cancer spheres were co-cultivated with human platelets. TF, EMT and stem cell marker levels were determined by Western blotting, flow cytometry and RT-PCR. Cancer cell migration was determined by Boyden chambers and the scratch assay.ResultsThe co-culture of patient-derived ovarian cancer cells with platelets causes: 1) a phenotypic change in cancer cells, 2) chemoattraction and cancer cell migration, 3) induced MIC markers (EMT/stemness), 3) increased sphere formation and 4) increased TF protein levels and activity.ConclusionsWe present the first evidence that platelets act as chemoattractants to cancer cells. Furthermore, platelets promote the formation of ovarian cancer spheres that express MIC markers and the metastatic protein TF. Our results suggest that platelet-cancer cell interaction plays a role in the formation of metastatic foci.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-015-1304-z) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

Platelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Structural and functional identification of vasculogenic mimicry in vitro

Racordon

Valdivia

Mingo

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in an endothelial cell-free manner. Despite its strong correlation with reduced patient survival, controversy still surrounds the existence of an in vitro model of VM. Furthermore, many studies that claim to demonstrate VM fail to provide solid evidence of true hollow channels, raising concerns as to whether actual VM is actually being examined. Herein, we provide a standardized in vitro assay that recreates the formation of functional hollow channels using ovarian cancer cell lines, cancer spheres and primary cultures derived from ovarian cancer ascites. X-ray microtomography 3D-reconstruction, fluorescence confocal microscopy and dye microinjection conclusively confirm the existence of functional glycoprotein-rich lined tubular structures in vitro and demonstrate that many of structures reported in the literature may not represent VM. This assay may be useful to design and test future VM-blocking anticancer therapies.

show abstract

Potencial herramienta en la personalización del tratamiento oncológico: Casos clínicos

Abstract: In vitro cell response to chemotherapeutic agents, to personalize ovarian cancer treatment.Report of two cases (Rev Med Chile 2013; 141: 669-673).

Cited by 2 publications

References 12 publications

Platelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells

Platelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells

Structural and functional identification of vasculogenic mimicry in vitro

Contact Info

Product

Resources

About