J. Neurosci. Res. volume 86, issue 13, P2925-2931 2008 DOI: 10.1002/jnr.21733 View full text
Huafei Lu, Gangwei Wei, Dongmin Wang, Patrick Yung, Weihai Ying

Abstract: DNA fragmentation and nuclear condensation are important nuclear changes in apoptosis. In this study we determined whether DNA fragmentation and nuclear condensation occur in astrocytes treated with 100-200 microM of the genotoxic agent M-nitroso-N-nitroguanidine (MNNG). Our study also investigated the roles of Ca(2+)-Mg(2+)-dependent endonuclease (CME) in the MNNG-induced nuclear changes. We found that MNNG induced profound ATP depletion as well as marked nuclear condensation and DNA fragmentation in the cell…

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