2009
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Abstract: Stroke in the neonatal brain is an understudied cause of neurologic morbidity. Recently we have characterized a new immature mouse model of stroke utilizing unilateral carotid ligation alone to produce infarcts and acute seizures in postnatal day 12 (P12) CD-1 mice. In this study, the amount of poststroke neural progenitor proliferation was examined in the subgranular (SGZ) of the dentate gyrus and the subventricular zone (SVZ) 7, 14, and 21 days after ischemia (DAI). A single IP injection (50 mg/kg) of bromod… Show more

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“…Chen et al (Chen et al, 2009) corroborated these decreases in hippocampal neurogenesis after chronic administration to neonatal rats, and also reported a conservation of the relative proportions of newborn neuronal and glial cell types. It is possible that these are model-dependent differences: the experiments cited previously were performed in naïve rodents, while our ischemia model has already demonstrated suppressed neurogenesis in the DG after the stroke event (Kadam et al, 2008; Kadam et al, 2009a). It is also possible that a single dose is not enough to induce these changes in neurogenesis in the period investigated in this study.…”
Section: Discussionmentioning
“…This model has been demonstrated to produce acute behavioral seizures, functional deficits, impaired neurogenesis and brain injury (Comi et al, 2004; Kadam et al, 2008; Kadam et al, 2009a; Kadam et al, 2009b). We report here the impact of a single acute dose of phenobarbital (30 mg/kg or 60 mg/kg) upon these endpoints after stroke in the immature brain.…”
Section: Introductionmentioning
“…Images inverted in Adobe Photoshop CS4 software (Adobe Systems Incorporated) granularly segmented to separate GFAP-positive area from GFAP-negative area and then the immunopositive area in each region of interest was measured ( Stacks of 1 mm thick images of BrdU and GFAP in the subventricular zone (SVZ) region were taken on an Olympus FV 1000 laser scanning confocal system at bregma coordinates level 0.74 to 1.18 mm [12]. Images were analyzed using Image J (U.S. National Institutes of Health; http://rsb.info.nih.gov/ij/, 1997-2006) software to arbitrarily quantify BrdU, and GFAP expression, in the region of the SVZ of ligated and control brains as previously reported [13]. The SVZ region was further subdivided into the SVZ proper, the white matter above the SVZ, and the striatum below the SVZ, and BrdU and GFAP labeling was quantified in these regions using MCID; standardized distances from the SVZ (Fig.…”
Section: Systemic Injection Of Cd34mentioning
“…In its place, we measured the area of the packed BrdU-positive cells in the SVZ horn (ie, using the green channel for BrdU-positive cells only; Fig. 2A), the expansion of which has been shown to represent increased proliferation in many rodent models of brain development, injury, and inflammation 1416. SVZ areas (ie detected by borders of tightly packed BrdU-labeled cells) were significantly larger in the MCAD-IgG-treated group than in both the MUC IgG and PBS controls.…”
Section: Resultsmentioning
“…This is consistent with the lentivirus findings that show a marked reduction of GFP+ cells in the injured SVZ compared to controls. The literature offers contrasting data regarding BrdU-labeled cells in the SVZ in the first 2-3 weeks following the injury with reports of an increased [1,2,4] , unchanged [29,30] or decreased amount of labeled cells [27] . Data differ depending on the age of the animal, the severity of the insult and the ischemia model used.…”
Section: Discussionmentioning