Postembryonic development of the dorsal longitudinal flight muscle and its innervation inManduca sexta

Duch, Carsten; Bayline, Ronald J.; Levine, Richard B.

doi:10.1002/(sici)1096-9861(20000619)422:1<1::aid-cne1>3.0.co;2-s

Cited by 46 publications

(50 citation statements)

References 54 publications

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“…Three techniques were used. The first consisted of Propidium Iodide staining of whole mount preparations (Duch et al 2000). The antenniform leg was clipped distal to the patella, and dissected in Schneider's culture medium at room temperature (RT).…”

Section: Methodsmentioning

confidence: 99%

Anatomy and Physiology of Giant Neurons in the Antenniform Leg of the Amblypygid Phrynus Marginemaculatus

Spence¹,

Hebets

2006

Journal of Arachnology

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Section: Methodsmentioning

confidence: 99%

Anatomy and Physiology of Giant Neurons in the Antenniform Leg of the Amblypygid Phrynus Marginemaculatus

Spence¹,

Hebets

2006

Journal of Arachnology

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“…The next increase in msslo message occurs approximately midway through adult development (P8-P10), temporally correlated with significant increases in muscle mass, differentiation, more extensive terminal innervation and the onset of muscle membrane excitability (Fig.·11) (Duch et al, 2000;Rheuben and Kammer, 1980). Nerve-muscle interactions are critical in the development of flight muscles in Drosophila (Fernandes and Keshishian, 1998) and in Manduca sexta (Bayline et al, 2001), so if msSlo currents are present in the developing DLMs, they may play an active role in these processes.…”

Section: Are Developmental Changes In Msslo Expression In Visceral Mumentioning

confidence: 97%

“…We chose to compare this regional set of skeletal muscles because the adult thoracic DLMs that are formed from a larval template as well as newly generated muscle (Duch et al, 2000) change from tonic to phasic flight muscles during metamorphosis (Rheuben and Kammer, 1980), while the abdominal muscles persist until after the moth ecloses and their muscle properties are thought not to change (Rheuben and Kammer, 1980;Truman et al, 1992). Although transcript levels in the DLMs in both regions transiently declined at P6, there was a marked regional difference in msslo mRNA levels in the adult tissue (Figs·8,·9).…”

Section: Msslo Expression Is Dramatically Upregulated In Skeletal Musmentioning

confidence: 99%

“…Steroid-mediated transcriptional control of ion channel gene expression could alter the electrical properties of larval neurons and muscles to tailor their electrical properties for new adult circuits and behavior. Developmental changes in potassium currents occur in Manduca leg (Hayashi and Levine, 1992;Grüenwald and Levine, 1998), flight motoneurons (Duch et al, 2000;Hayashi and Levine, 1992), antennal lobe neurons (Mercer and Hildebrand, 2002a,b) and glia (Lohr et al, 2001), but the ion channel genes or gene products producing these potassium currents are not known yet.…”

Section: Steroid Hormone Modulation Of Ion Channel Gene Expressionmentioning

confidence: 99%

“…Manduca sexta slowpoke raise the possibility that functional msSlo currents may be present and participate in early as well as late neuromuscular development of the flight system. The first increase in msslo transcript in the DLMs occurs during the wandering stage and is concurrent with the onset of degeneration of larval muscle fibers, retraction of the innervating nerve terminals and myoblast proliferation (Fig.·11) (Duch et al, 2000). BK channels can participate in programmed cell death in vascular smooth muscle (Krick et al, 2001), and potassium efflux is a major component in neuronal apoptosis (Yu, 2003;Yu et al, 1997).…”

Section: Are Developmental Changes In Msslo Expression In Visceral Mumentioning

confidence: 99%

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Calcium-activated potassium channel of the tobacco hornworm,Manduca sexta: molecular characterization and expression analysis

Keyser

Witten

2005

Journal of Experimental Biology

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Large-conductance calciumand voltage-gated potassium channels (BK or Slowpoke) serve as dynamic integrators linking electrical signaling and intracellular activity. These channels can mediate many different Ca 2+ -dependent physiological processes including the regulation of neuronal and neuroendocrine cell excitability and muscle contraction. To gain insights into the function of BK channels in vivo, we isolated a full-length cDNA encoding the alpha subunit of a Slowpoke channel from the tobacco hornworm, Manduca sexta (msslo). Amino acid sequence comparison of the deduced Manduca protein revealed at least 80% identity to the insect Slo channels. The five C-terminal alternative splice regions are conserved, but the cloned cDNA fragments contained some unique combinations of exons E, G and I. Our spatial profile revealed that transcript levels were highest in skeletal muscle when compared with the central nervous system (CNS) and visceral muscle. The temporal profile suggested that msslo expression is regulated developmentally in a tissue-and regional-specific pattern. The levels of msslo transcripts remain relatively constant throughout metamorphosis in the CNS, transiently decline in the heart and are barely detectable in the gut except in adults. A dramatic upregulation of msslo transcript levels occurs in thoracic but not abdominal dorsal longitudinal body wall muscles (DLM), suggesting that the msSlo current plays an important role in the excitation or contractile properties of the phasic flight muscle. Our developmental profile of msslo expression suggests that msSlo currents may contribute to the changes in neural circuits and muscle properties that produce stage-specific functions and behaviors.

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Remodeling of the femoral chordotonal organ during metamorphosis of the hawkmoth,Manduca sexta

2000

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During metamorphosis of the moth, Manduca sexta, the larval legs degenerate and are replaced by adult legs with a diverse array of new sensory organs. The majority of the larval sensory neurons degenerate but some hair sensilla and chordotonal organ sensory neurons survive metamorphosis (Consoulas [2000] J. Comp. Neurol. 419:154-174). In the present study nerve-tracing techniques, birth-date labeling (5-bromodeoxyuridine), and electrophysiology were used to describe the remodeling of the femoral chordotonal organ (FCO) in the prothoracic legs. The larval FCO is composed of two scoloparia, which are associated with separate apodemes. At the onset of metamorphosis, some of the 13 larval neurons degenerate, together with the larval FCO apodemes. The remaining larval FCO sensory neurons persist in the imaginal leg to become the precursors of the adult femoral and tibial chordotonal organs respectively. Early in the pupal stage, 45 to 60 new sensory neurons are generated de novo and become associated with 6 persistent larval neurons in the imaginal femur to compose the adult FCO. Two clusters of persistent and new neurons are enclosed into two scoloparia with short apodemes that eventually become fused. In both larval and adult stages, the FCO contains units that respond phasically and others that respond tonically to femorotibial movements. Nerve activity from the FCO neurons can be recorded continuously during the remodeling of the organ. A persistent leg flexor motoneuron receives inputs from the FCO sensory neurons in both larval and adult stages, offering the opportunity to investigate the remodeling of the neural circuits underlying the proprioceptive control of the femorotibial joint.

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Postembryonic development of the dorsal longitudinal flight muscle and its innervation inManduca sexta

Cited by 46 publications

References 54 publications

Anatomy and Physiology of Giant Neurons in the Antenniform Leg of the Amblypygid Phrynus Marginemaculatus

Anatomy and Physiology of Giant Neurons in the Antenniform Leg of the Amblypygid Phrynus Marginemaculatus

Calcium-activated potassium channel of the tobacco hornworm,Manduca sexta: molecular characterization and expression analysis

Remodeling of the femoral chordotonal organ during metamorphosis of the hawkmoth,Manduca sexta

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