Post-Translational Modifications Drive Success and Failure of Fungal–Host Interactions

Retanal, Charmaine; Ball, Brianna; Geddes‐McAlister, Jennifer

doi:10.3390/jof7020124

Cited by 32 publications

(21 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The virulence of plant pathogens have been reported to be partially controlled by epigenetic mechanisms, such as DNA methylation and histone modifications, because these modifications change protein structure and function; therefore, the fungal development and pathogenicity also could be regulated for fungal survival during infection. PTMs modulate not only protein production and activity but also the host response ( Dubey and Jeon, 2017 ; Kong et al, 2018 ; Nai et al, 2020 ; Retanal et al, 2021 ). Khib has recently been reported in F. oxysporum , B. cinerea , and U. virens .…”

Section: Discussionmentioning

confidence: 99%

Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts

Xu¹,

Zhang²,

Yu³

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Aspergillus niger is a very destructive pathogen causing severe peanut root rot, especially in the seeding stage of peanuts (Arachis hypogaea), and often leading to the death of the plant. Protein lysine 2-hydroxyisobutyrylation (Khib) is a newly detected post-translational modification identified in several species. In this study, we identified 5041 Khib sites on 1,453 modified proteins in A. niger. Compared with five other species, A. niger has conserved and novel proteins. Bioinformatics analysis showed that Khib proteins are widely distributed in A. niger and are involved in many biological processes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that Khib proteins were significantly enriched in many cellular compartments and pathways, such as ribosomes and proteasome subunits. A total of 223 Khib proteins were part of the PPI network, thus, suggesting that Khib proteins are associated with a large range of protein interactions and diverse pathways in the life processes of A. niger. Several identified proteins are involved in pathogenesis regulation. Our research provides the first comprehensive report of Khib and an extensive database for potential functional studies on Khib proteins in this economically important fungus.

show abstract

Section: Discussionmentioning

confidence: 99%

Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts

Xu¹,

Zhang²,

Yu³

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The fungal cell wall plays an essential role during the host-pathogen interaction, besides participating in the cell wall integrity and remodeling [ 13 ]. Several pathogen-associated molecular patterns (PAMPs) can be found in the cell surface, which might be recognized through pattern recognition receptors (PRRs) of the host immune system, or which function as virulence factors, participating during the infection with a role in the organism pathogenicity [ 1 , 14 , 15 ].…”

Section: Cell Wall Proteinsmentioning

confidence: 99%

“…In pathogenic fungi, many glycoproteins participate during the infection, having a role in how the pathogen invades the host, promotes its survival, and evades the host immune response [ 14 , 15 ]. Therefore, protein secretion is important for the pathogenic processes, and can take place by three main mechanisms: (i) co-translational translocation, through the endoplasmic reticulum and Golgi apparatus, which requires the presence of a signal sequence (signal peptide) in the N-terminus of the protein; (ii) post-translational translocation, which is SRP (signal recognition particle)-independent; and (iii) as cargo of extracellular vesicles (EVs) [ 17 , 18 , 19 ].…”

Section: Cell Wall Proteinsmentioning

confidence: 99%

Virulence Factors of Sporothrix schenckii

García-Carnero

Martínez-Álvarez

2022

JoF

View full text Add to dashboard Cite

Sporothrix schenckii is one of the etiological agents of sporotrichosis. In this review, we discuss the virulence factors that have been proven to participate in the S. schenckii-host interaction. Among these known factors, we can find cell wall glycoproteins, adhesins, melanin, extracellular vesicles, and dimorphism. Furthermore, the morphological transition of S. schenckii in response to environmental conditions such as pH and temperature represents a means by which the fungus is able to establish mycosis in mammals. One of the key features in the development of sporotrichosis is the adhesion of the fungus to the host extracellular matrix. This event represents the first step to developing the mycosis, which involves adhesins such as the glycoproteins Gp70, Hsp60, and Pap1, which play a key role during the infection. The production of melanin helps the fungus to survive longer in the tissues and to neutralize or diminish many of the host’s attacks, which is why it is also considered a key factor in pathogenesis. Today, the study of human fungal pathogens’ virulence factors is a thriving area of research. Although we know some of the virulence factors in S. schenckii, much remains to be understood about the complex process of sporotrichosis development and the factors involved during the infection.

show abstract

“…Bottom-up mass spectrometry-based proteomics of disease, the focus of this review, provides insight into disease mechanisms and the host's ability to fight against infection, along with the discovery and characterization of novel drug targets [19,20]. For example, the role of transition metal acquisition (e.g., zinc) on the intracellular and extracellular environments of Cryptococcus neoformans was recently explored using quantitative proteomics [21].…”

Section: Introductionmentioning

confidence: 99%

Proteomics of Cryptococcus neoformans: From the Lab to the Clinic

Muselius

Durand

Geddes‐McAlister

2021

IJMS

Self Cite

View full text Add to dashboard Cite

Fungal pathogens cause an array of diseases by targeting both immunocompromised and immunocompetent hosts. Fungi overcome our current arsenal of antifungals through the emergence and evolution of resistance. In particular, the human fungal pathogen, Cryptococcus neoformans is found ubiquitously within the environment and causes severe disease in immunocompromised individuals around the globe with limited treatment options available. To uncover fundamental knowledge about this fungal pathogen, as well as investigate new detection and treatment strategies, mass spectrometry-based proteomics provides a plethora of tools and applications, as well as bioinformatics platforms. In this review, we highlight proteomics approaches within the laboratory to investigate changes in the cellular proteome, secretome, and extracellular vesicles. We also explore regulation by post-translational modifications and the impact of protein–protein interactions. Further, we present the development and comprehensive assessment of murine models of cryptococcal infection, which provide valuable tools to define the dynamic relationship between the host and pathogen during disease. Finally, we explore recent quantitative proteomics studies that begin to extrapolate the findings from the bench to the clinic for improved methods of fungal detection and monitoring. Such studies support a framework for personalized medical approaches to eradicate diseases caused by C. neoformans.

show abstract

Post-Translational Modifications Drive Success and Failure of Fungal–Host Interactions

Cited by 32 publications

References 98 publications

Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts

Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus niger in Peanuts

Virulence Factors of Sporothrix schenckii

Proteomics of Cryptococcus neoformans: From the Lab to the Clinic

Contact Info

Product

Resources

About