Post-screening characterisation and in vivo evaluation of an anti-inflammatory polysaccharide fraction from Eucommia ulmoides

Li, Qiu; Yao, Feng; He, Wei; Wang, Lintao; Wang, Ruibing; Dong, Lei; Wang, Chunming

doi:10.1016/j.carbpol.2017.04.034

Cited by 58 publications

(34 citation statements)

References 52 publications

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“…The sample concentration was 2 mg mL À1 . The MW was determined from a calibration curve generated using a series of polysaccharide standards (Sigma) with definite molecular masses ranging from 10 to 600 kDa (Li, et al, 2017).…”

Section: Homogeneity and Molecular Weight Determinationmentioning

confidence: 99%

“…The acid hydrolysis was performed by using trifluoroacetic acid (TFA) at different concentrations to analyse the monosaccharide composition of the backbone and branch chains (Li, et al, 2017). The methylated sample was dissolved in 3 mL of 88% formic acid solution, blocked and depolymerised at 100°C for 3 h. The hydrolysate was concentrated to dryness under reduced pressure, followed by addition of 3 mL of methanol to the reaction flask, and the mixture was evaporated to dryness at 40°C.…”

Section: Degradation and Reduction Of Fully Methylated Samplesmentioning

confidence: 99%

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The structural studies of a polysaccharide purified from Oat Lao‐Chao

Rong

Zhang

et al. 2020

Int J of Food Sci Tech

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Oat Lao-Chao contains bioactive components such as polysaccharides, of which immunomodulatory activity is recognised as the most important biological activity of polysaccharides. This paper studied optimisation of manufacturing Oat Lao-Chao, extraction of polysaccharide and removal of its protein, separation and purification of polysaccharide. The results showed that a water-soluble polysaccharide coded as LCPS-2 was isolated from Oat Lao-Chao. The polysaccharide was homogeneous and produced an asymmetrical peak obtained by high-performance gel permeation chromatography. Monosaccharide analysis revealed that LCPS-2 was containing arabinose, xylose, galactose, glucose and mannose in a ratio of 1.

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Section: Homogeneity and Molecular Weight Determinationmentioning

confidence: 99%

Section: Degradation and Reduction Of Fully Methylated Samplesmentioning

confidence: 99%

The structural studies of a polysaccharide purified from Oat Lao‐Chao

Rong

Zhang

et al. 2020

Int J of Food Sci Tech

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“…3,4 However, natural polysaccharides can induce RAW264.7 macrophages to express IL-10 and change macrophages into M2 phenotypes to maintain body health. 5 Therefore, macrophages are oen used as cell models to evaluate inammatory responses.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato

et al. 2020

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“…A multitude of monosaccharide units bonded through glycosidic linkages constitute polysaccharides, which frequently exist in plants, bacteria, fungi and animals, as a vital biological macromolecule. The low toxicity associated with polysaccharides, together with their wide-ranging bioactivities, like antidiabetic [ 1 ], anti-inflammatory [ 2 , 3 ], antitumor [ 4 ], antioxidation [ 5 , 6 ], antiobesity [ 7 ] and immunomodulation [ 8 , 9 ] have contributed towards their increasing popularity in the scientific community. Similarly, immunostimulatory polysaccharides can directly or indirectly activate the immune system by triggering several cellular or molecular events [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Physicochemical Characteristics and Macrophage Immunostimulatory Activities of Polysaccharides from Chlamys farreri

Shi

Liu

et al. 2020

Marine Drugs

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To address the structure–activity relationship of Chlamys farreri polysaccharides on their immunostimulatory efficacy, two polysaccharides (CFP-1 and CFP-2) were extracted from Chlamys farreri by hot water extraction, and separated through column chromatography. The isolated CFPs were chemically analyzed to clarify their physicochemical characteristics and cultured with murine macrophage RAW264.7 cells, in order to evaluate their immunostimulatory efficacy. Despite the fact that both CFP-1 and CFP-2 were mainly comprised of glucose lacking the triple-helix structure, as revealed through preliminary physicochemical analyses, obvious differences in regard to molecular weight (Mw), glucuronic acid content (GAc) and branching degree (BD) were observed between CFP-1 and CFP-2. In in vitro immunostimulatory assays for macrophage RAW264.7 cells, it was demonstrated that CFP-2 with larger Mw, more GAc and BD could evidently promote phagocytosis and increase the production of NO, IL-6, TNF-α and IL-1β secretion, by activating the expression of iNOS, IL-6, TNF-α and IL-1β genes, respectively. Hence, CFP-2 shows great promise as a potential immunostimulatory agent in the functional foods and nutraceutical industry, while CFP-1, with lower molecular weight, less GAc and BD, displays its weaker immunostimulatory efficacy, based on the indistinctive immunostimulatory parameters of CFP-1.

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Post-screening characterisation and in vivo evaluation of an anti-inflammatory polysaccharide fraction from Eucommia ulmoides

Cited by 58 publications

References 52 publications

The structural studies of a polysaccharide purified from Oat Lao‐Chao

The structural studies of a polysaccharide purified from Oat Lao‐Chao

Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato

Comparison of Physicochemical Characteristics and Macrophage Immunostimulatory Activities of Polysaccharides from Chlamys farreri

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