Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae

Coutinho, Pedro M.; Andersen, Mikael Rørdam; Kolenová, Katarína; vanKuyk, Patricia A.; Benoit, Isabelle; Gruben, Birgit S.; Trejo-Aguilar, Blanca; Visser, Hans; Solingen, Piet van; Pakula, Tiina; Seiboth, Bernard; Battaglia, Evy; Aguilar-Osorio, Guillermo; Jong, Jan F. de; Ohm, Robin A.; Aguilar, Mariana; Henrissat, Bernard; Nielsen, Jens; Stålbrand, Henrik; Vries, Ronald P. de

doi:10.1016/j.fgb.2008.07.020

Cited by 137 publications

(115 citation statements)

References 62 publications

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“…Among ascomycetes, the genome of P. anserina revealed the potential of this coprophilic fungus to hydrolyze recalcitrant lignocellulosic residues (19). It contains a significantly higher number of putative cellulases and xylanases compared to A. niger, A. nidulans and A. oryzae, reflected by its growth profile, which is better than Aspergillus species on cellulose and xylan (15). These observations fit well with the natural biotope of P. anserina, which is a late colonizer of herbivore dung, in which lignocellulose is the main remaining organic component.…”

Section: Discussionmentioning

confidence: 99%

Podospora anserina Hemicellulases Potentiate the Trichoderma reesei Secretome for Saccharification of Lignocellulosic Biomass

Couturier

Haon²,

Coutinho

et al. 2011

Appl Environ Microbiol

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To improve the enzymatic hydrolysis (saccharification) of lignocellulosic biomass by Trichoderma reesei, a set of genes encoding putative polysaccharide-degrading enzymes were selected from the coprophilic fungus Podospora anserina using comparative genomics. Five hemicellulase-encoding genes were successfully cloned and expressed as secreted functional proteins in the yeast Pichia pastoris. These novel fungal CAZymes belonging to different glycoside hydrolase families (PaMan5A and PaMan26A mannanases, PaXyn11A xylanase, and PaAbf51A and PaAbf62A arabinofuranosidases) were able to break down their predicted cognate substrates. Although PaMan5A and PaMan26A displayed similar specificities toward a range of mannan substrates, they differed in their end products, suggesting differences in substrate binding. The N-terminal CBM35 module of PaMan26A displayed dual binding specificity toward xylan and mannan. PaXyn11A harboring a C-terminal CBM1 module efficiently degraded wheat arabinoxylan, releasing mainly xylobiose as end product. PaAbf51A and PaAbf62A arabinose-debranching enzymes exhibited differences in activity toward arabinose-containing substrates. Further investigation of the contribution made by each P. anserina auxiliary enzyme to the saccharification of wheat straw and spruce demonstrated that the endo-acting hemicellulases (PaXyn11A, PaMan5A, and PaMan26A) individually supplemented the secretome of the industrial T. reesei CL847 strain. The most striking effect was obtained with PaMan5A that improved the release of total sugars by 28% and of glucose by 18%, using spruce as lignocellulosic substrate.Lignocellulosic biomass is a desirable feedstock for the supply of second-generation bioethanol, being the largest renewable source of carbohydrates, but the recalcitrance of raw materials makes biotechnological conversion complex and costly (34).The main components of plant cell walls are cellulose, lignin, and hemicellulose, which form a tight complex with varying proportions between plants (for a review, see reference 45). Cellulose microfibrils are formed by crystalline-like combination of linear and highly ordered polymer chains of ␤-1,4-linked D-glucose residues, and lignin consists of an amorphous aromatic heteropolymer with hydrophobic properties. Hemicellulose is a combined designation of a diverse set of noncrystalline carbohydrate polymers from plant tissues that form closely associated networks with cellulose microfibrils and lignin (6, 24). Typically, the most abundant component of hemicellulose in the cell walls of monocots (e.g., cereals) is ␤-1,4-xylan, which consists of ␤-1,4-linked D-xylose residues substituted with L-arabinosyl, 4-O-methyl-glucuronosyl and acetyl side chains (46,53). Arabinofuranosyl residues of arabinoxylan may also be esterified with hydroxycinnamic acid residues, e.g., ferulic and p-coumaric acids (53). In softwoods (gymnosperms), the main hemicellulose component is ␤-1,4-mannan, which consists of a backbone formed by ␤-1,4-linked D-mannose and D-glucose residues substitu...

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Section: Discussionmentioning

confidence: 99%

Podospora anserina Hemicellulases Potentiate the Trichoderma reesei Secretome for Saccharification of Lignocellulosic Biomass

Couturier

Haon²,

Coutinho

et al. 2011

Appl Environ Microbiol

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“…Analysis of its genome [15] in combination with proteomic studies [9,16] show it to have a similar polysaccharide degradation potential to that of the industrial fungi, and in addition the regulatory mechanisms involved in controlling the expression of plant cell wall degrading activities are mostly conserved between it and industrially important fungi [17][18][19][20]. Finally, the use of A. nidulans as a production vehicle could provoke less concern regarding mycotoxin synthesis since its genome encodes the metabolic pathway for the less toxic compound sterigmatocystin compared to certain industrial strains which still retain the capacity to produce very harmful mycotoxins [21,22].…”

Section: Introductionmentioning

confidence: 99%

Enhanced glycosyl hydrolase production in Aspergillus nidulans using transcription factor engineering approaches

Tamayo-Ramos

Orejas

2014

Biotechnol Biofuels

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Background: Engineered fungi are attractive platforms for the production of plant cell wall hydrolytic enzymes which, among other biotechnological applications, are required for the efficient conversion of biomass to glucose and other fermentable sugars. As a fungal model system, Aspergillus nidulans provides genetic tools that are of relevance in this context and potentially applicable to industrially important filamentous fungi. The goal of this study is to assess the utility of A. nidulans as a host for recombinant protein production.

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“…We further assessed the phenotype of 34 strains containing deletions of predicted transcription factor (TF) genes that showed induction when N. crassa is exposed to xylan and identified a predicted ortholog of xlnR/xyr1 (NCU06971; xlr-1). In Hypocrea jecorina (Trichoderma reesei) and Aspergillus spp., the transcriptional regulator XYR1/XlnR, respectively, regulates expression of both hemicellulase and cellulase genes (12,31,34,38,51,52,61). Lossof-function mutants in xlnR in Aspergillus niger exhibit strongly reduced xylanolytic activities (64).…”

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confidence: 99%

Deciphering Transcriptional Regulatory Mechanisms Associated with Hemicellulose Degradation in Neurospora crassa

et al. 2012

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ABSTRACTHemicellulose, the second most abundant plant biomass fraction after cellulose, is widely viewed as a potential substrate for the production of liquid fuels and other value-added materials. Degradation of hemicellulose by filamentous fungi requires production of many different enzymes, which are induced by biopolymers or its derivatives and regulated mainly at the transcriptional level through transcription factors (TFs).Neurospora crassa, a model filamentous fungus, expresses and secretes enzymes required for plant cell wall deconstruction. To better understand genes specifically associated with degradation of hemicellulose, we applied secretome and transcriptome analysis toN. crassagrown on beechwood xylan. We identified 34 secreted proteins and 353 genes with elevated transcription on xylan. The xylanolytic phenotype of strains with deletions in genes identified from the secretome and transcriptome analysis of the wild type was assessed, revealing functions for known and unknown proteins associated with hemicellulose degradation. By evaluating phenotypes of strains containing deletions of predicted TF genes inN. crassa, we identified a TF (XLR-1;xylan degradationregulator1) essential for hemicellulose degradation that is an ortholog to XlnR/XYR1 inAspergillusandTrichodermaspecies, respectively, a major transcriptional regulator of genes encoding both cellulases and hemicellulases. Deletion ofxlr-1inN. crassaabolished growth on xylan and xylose, but growth on cellulose and cellulolytic activity were only slightly affected. To determine the regulatory mechanisms for hemicellulose degradation, we explored the transcriptional regulon of XLR-1 under xylose, xylanolytic, and cellulolytic conditions. XLR-1 regulated only some predicted hemicellulase genes inN. crassaand was required for a full induction of several cellulase genes. Hemicellulase gene expression was induced by a combination of release from carbon catabolite repression (CCR) and induction. This systematic analysis illustrates the similarities and differences in regulation of hemicellulose degradation among filamentous fungi.

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Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae

Cited by 137 publications

References 62 publications

Podospora anserina Hemicellulases Potentiate the Trichoderma reesei Secretome for Saccharification of Lignocellulosic Biomass

Podospora anserina Hemicellulases Potentiate the Trichoderma reesei Secretome for Saccharification of Lignocellulosic Biomass

Enhanced glycosyl hydrolase production in Aspergillus nidulans using transcription factor engineering approaches

Deciphering Transcriptional Regulatory Mechanisms Associated with Hemicellulose Degradation in Neurospora crassa

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