Post‐fertilization effects of chronic renal failure in male rats

Abstract

We evaluated the potential for growth and intrauterine development of embryos generated from the fertilization of oocytes with spermatozoa recovered from animals with chronic renal failure (CRF). Group A included sham-operated rats (n = 28), group B1 involved CRF rats that had undergone erythropoietin plus bromocryptine treatment (n = 28), and group B2 included CRF rats that had received normal saline. Embryos derived from the in vitro fertilization of oocytes with spermatozoa recovered from rats of group A or group B1 or group B2 were transferred to female recipients. We induced CRF in a group of rats (group B; n = 56; the total kidney volume was reduced to one-sixth with two operations). One week after the second operation, the rats of group B were randomly divided into group B1 (they subsequently received bromocryptine plus erythropoietin) and group B2 (they received injections of saline). Nine weeks after the second operation, the fertility of each male rat was assessed by mating tests and in vitro fertilization of oocytes. The mean litter size was significantly smaller in the subpopulation of fertile animals in group B2 than in the fertile rats of group B1 and in the fertile rats of group B1 than in the fertile rats of group A. Per cent of transferred blastocysts that developed into alive offspring were significantly lower in group B2 than in group B1 and in group B1 than in group A. Epididymal spermatozoa demonstrated a significantly larger DNA-oxidative damage in group B2 than in group B1 and in group B1 than in group A. These findings demonstrate that sperm-DNA damage because of CRF development is accompanied by a defect in the development of embryos generated in vitro. We may suggest that bromocryptine and erythropoietin protecting sperm DNA from oxidative damage improve reproductive potential in rats with CRF.

Efficacy and safety of varicocelectomies: A meta-analysis

“…The mission of the male gamete is not only to fertilize the oocyte but also to transfer its information to the oocyte. This is essential to early embryonic development and embryonic capacity for implantation [Dimitriadis et al 2009a;Dimitriadis et al 2009b;Tsounapi et al 2016]. It would be of clinical significance to compare the live birth rates additionally to the pregnancy rates among the three surgical approaches.…”

Effects of phosphodiesterase‐5 inhibitors on Leydig cell secretory function in oligoasthenospermic infertile men: a randomized trial

“…For each quantitative variable Bartlett's test of homogeneity of variance was used to determine if the groups had equivalent variances; if the variances were equivalent, the groups were compared by one-way ANOVA , and if there were significant differences by ANOVA , then Duncan's multiple comparison test was used. If the groups did not have equivalent variance in one variable, the Kruskal-Wallis one-way ANOVA was used to assess the overall effects, as previously described [14]. In this case, whenever differences were significant, pair-wise comparisons were made using the Mann-Whitney U -test.…”

Sperm DNA Damage Before and After Kidney Transplantation

“…A study by Yu et al showed that renal transplantation could improve spermatogenesis in uremic patients (22). The results of another study on rats with chronic renal failure who received bromocryptine plus to erythropoietin showed that these drugs could protect sperm DNA from oxidative damage and improve reproductive function (23).…”