Possible Metsulfuron Herbicide Detoxification by a Oryza sativa L. Glutathione S-transferase Enzyme.

Amador, Vinícius Costa; Silva, Edson Ferreira da; Nadvorny, Daniela; Maia, Rafael Trindade

doi:10.1590/1678-4324-2020180571

Cited by 5 publications

(6 citation statements)

References 33 publications

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“…Another large portion of the studied proteins responsible for glutathione metabolism was observed and the amounts of these proteins were higher in the herbicide-treated sample and in F. culmorum -inoculated wheat. Plant glutathione transferase (theoretical MW 24,984 Da, in gel ~24 kDa), an enzyme capable of catalyzing the conjugation and detoxification of herbicides, was found in higher amounts in all 2,4-D-treated cultures in this study [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Mironenka

Różalska

Bernat

2021

IJMS

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Wheat is a critically important crop. The application of fungi, such as Trichoderma harzianum, to protect and improve crop yields could become an alternative solution to synthetic chemicals. However, the interaction between the fungus and wheat in the presence of stress factors at the molecular level has not been fully elucidated. In the present work, we exposed germinating seeds of wheat (Triticum aestivum) to the plant pathogen Fusarium culmorum and the popular herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of T. harzianum or its extracellular metabolites. Then, the harvested roots and shoots were analyzed using spectrometry, 2D-PAGE, and MALDI-TOF/MS techniques. Although F. culmorum and 2,4-D were found to disturb seed germination and the chlorophyll content, T. harzianum partly alleviated these negative effects and reduced the synthesis of zearalenone by F. culmorum. Moreover, T. harzianum decreased the activity of oxidoreduction enzymes (CAT and SOD) and the contents of the oxylipins 9-Hode, 13-Hode, and 13-Hotre induced by stress factors. Under the influence of various growth conditions, changes were observed in over 40 proteins from the wheat roots. Higher volumes of proteins and enzymes performing oxidoreductive functions, such as catalase, ascorbate peroxidase, cytochrome C peroxidase, and Cu/Zn superoxide dismutase, were found in the Fusarium-inoculated and 2,4-D-treated wheat roots. Additionally, observation of the level of 12-oxo-phytodienoic acid reductase involved in the oxylipin signaling pathway in wheat showed an increase. Trichoderma and its metabolites present in the system leveled out the mentioned proteins to the control volumes. Among the 30 proteins examined in the shoots, the expression of the proteins involved in photosynthesis and oxidative stress response was found to be induced in the presence of the herbicide and the pathogen. In summary, these proteomic and metabolomic studies confirmed that the presence of T. harzianum results in the alleviation of oxidative stress in wheat induced by 2,4-D or F. culmorum.

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Section: Resultsmentioning

confidence: 99%

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Mironenka

Różalska

Bernat

2021

IJMS

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“…The alignment revealed that the H-site of GSTU7 contains a Met–Trp–Ala motif that is also present in OsGSTU4 from rice. The overexpression of OsGSTU4 in Arabidopsis has proven to generate more tolerant plants toward salt stress and MV-induced photooxidative stress, while in silico characterization led to the hypothesis that OsGSTU4 might detoxify the herbicide metsulfuron ( Sharma et al, 2014 ; Amador et al, 2020 ). These results together suggest a conserved function of distinct TAU GSTs and may provide leads for further analysis.…”

Section: Discussionmentioning

confidence: 99%

A dual role for glutathione transferase U7 in plant growth and protection from methyl viologen-induced oxidative stress

et al. 2021

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Plant glutathione S-transferases (GSTs) are glutathione-dependent enzymes with versatile functions, mainly related to detoxification of electrophilic xenobiotics and peroxides. The Arabidopsis (Arabidopsis thaliana) genome codes for 53 GSTs, divided into seven subclasses, however, understanding of their precise functions is limited. A recent study showed that class II TGA transcription factors TGA2, TGA5 and TGA6 are essential for tolerance of UV-B-induced oxidative stress and that this tolerance is associated with an antioxidative function of cytosolic tau-GSTs (GSTUs). Specifically, TGA2 controls the expression of several GSTUs under UV-B light, and constitutive expression of GSTU7 in the tga256 triple mutant is sufficient to revert the UV-B-susceptible phenotype of tga256. To further study the function of GSTU7, we characterized its role in mitigation of oxidative damage caused by the herbicide methyl viologen (MV). Under non-stress conditions, gstu7 null mutants were smaller than wild-type (WT) plants and delayed in the onset of the MV-induced antioxidative response, which led to accumulation of hydrogen peroxide and diminished seedling survival. Complementation of gstu7 by constitutive expression of GSTU7 rescued these phenotypes. Furthermore, live monitoring of the glutathione redox potential in intact cells with the fluorescent probe Grx1-roGFP2 revealed that GSTU7 overexpression completely abolished the MV-induced oxidation of the cytosolic glutathione buffer compared to WT plants. GSTU7 acted as a glutathione peroxidase able to complement the lack of peroxidase-type GSTs in yeast. Together, these findings show that GSTU7 is crucial in the antioxidative response by limiting oxidative damage and thus contributes to oxidative stress resistance in the cell.

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“…The detoxification mechanisms of xenobiotics are known to be a multi‐step process involving several proteins in each phase. The current knowledge of the toxicity response of xenobiotics can be divided into contaminant transformation (phase I), conjugation (phase II), and excretion (phase III), which are well‐conserved detoxification mechanisms in a wide range of organisms 23–26 . Initially, cytochrome P450 (CYP) transforms the xenobiotics into a more soluble compound, then glutathione S‐transferase (GST) conjugates the xenobiotics with glutathione (GSHs) and transports the contaminants into ATP‐binding cassette (ABC) transporters.…”

Section: Discussionmentioning

confidence: 99%

“…Phase I reactions convert herbicide into less active compounds by the cytochrome P450s enzymes (CYPs) 22,23 . Phase II reactions conjugate herbicide to other water‐soluble substances by glutathione S‐transferases (GSTs) 24 . Phase III reactions involve transporting conjugated glyphosate into the vacuole or out of the cell by ATP‐binding cassette (ABC) transporters 25,26 .…”

Section: Introductionmentioning

confidence: 99%

“…22,23 Phase II reactions conjugate herbicide to other water-soluble substances by glutathione S-transferases (GSTs). 24 Phase III reactions involve transporting conjugated glyphosate into the vacuole or out of the cell by ATP-binding cassette (ABC) transporters. 25,26 Phase IV reactions involve partial degradation and deposition of metabolites by carboxypeptidase (CPs).…”

Section: Introductionmentioning

confidence: 99%

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Glyphosate metabolism in Tetrahymena thermophila: A shotgun proteomic analysis approach

Manan

Roytrakul

Charoenlappanit

et al. 2023

Environmental Toxicology

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Glyphosate is one of the most widely used herbicides in the world. However, because of its overuse and resistance to degradation, high levels of glyphosate residues in the environment are reported. Therefore, this study aimed to investigate the effects of glyphosate on proteomic aspects of Tetrahymena thermophila and their uses as bioindicators of freshwater ecosystem. First, an acute toxicity test was performed to determine the median inhibition concentration (IC 50 ). The toxicity test results showed that glyphosate inhibited the growth (proliferation) of T. thermophila. The 96 h-IC 50 value of glyphosate was 171 mg L À1 . No visible changes in aggregation behavior and cell morphology were observed under glyphosate exposure. In addition, the effects of low and high dose glyphosate concentrations (77.5 mg L À1 , 171 mg L À1 ) on the proteomic changes of T. thermophila was investigated using a label-free shotgun proteomic approach. A total of 3191 proteins were identified, 2791 proteins were expressed in the control, 2651 proteins were expressed in 77.5 mg L À1 glyphosates, and 3012 proteins were expressed in 171 mg L À1 glyphosates. Under glyphosate exposure at both low and high dose glyphosate, 400 unique proteins were upregulated. The majority of these proteins was classified as proteins associated with oxidative stress response and intracellular transport indicating the shifts in the internal metabolism. Proteomics revealed that the glyphosate metabolism by T. thermophila is a multi-step process involving several enzymes, which can be divided into four phases, including modification (phase I), conjugation (phase II), transport (phase III), and degradation (phase IV). The accumulation of various biochemical reactions contributes to overall glyphosate resistance. With the proteomics approach, we have found that T. thermophila was equipped with glyphosate detoxification and degradation mechanisms.

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Possible Metsulfuron Herbicide Detoxification by a Oryza sativa L. Glutathione S-transferase Enzyme.

Cited by 5 publications

References 33 publications

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

Potential of Trichoderma harzianum and Its Metabolites to Protect Wheat Seedlings against Fusarium culmorum and 2,4-D

A dual role for glutathione transferase U7 in plant growth and protection from methyl viologen-induced oxidative stress

Glyphosate metabolism in Tetrahymena thermophila: A shotgun proteomic analysis approach

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