Possible mechanism for zinc protection against cadmium cytotoxicity in cultured vascular endothelial cells

Zinc diethyldithiocarbamate as an inducer of metallothionein in cultured vascular endothelial cells

“…After a 24-hr treatment with inorganic zinc, MT protein expression was not observed (Fig. 1A), consistent with our previous study (Kaji et al, 1992). Furthermore, ZnSO 4 did not alter the expression of MT-1A, MT-1E, or MT-2A mRNAs, MT subisoforms expressed in bovine tissue (Fig.…”

“…1D), suggesting that the zinc ion could not activate the MTF-1/MRE and Nrf2/ARE pathways, which regulate MT induction. Previously, we investigated the interaction between zinc and cadmium in vascular endothelial cells and found that zinc accumulates within the cells but does not induce MT at the protein level; however, zinc protects cells from cadmium cytotoxicity by decreasing the intracellular accumulation of cadmium (Kaji et al, 1992). In the present study, we confi rmed that ZnSO 4 could not induce MT protein or mRNA.…”

Zinc diethyldithiocarbamate as an inducer of metallothionein in cultured vascular endothelial cells

“…After a 24-hr treatment with inorganic zinc, MT protein expression was not observed (Fig. 1A), consistent with our previous study (Kaji et al, 1992). Furthermore, ZnSO 4 did not alter the expression of MT-1A, MT-1E, or MT-2A mRNAs, MT subisoforms expressed in bovine tissue (Fig.…”

“…1D), suggesting that the zinc ion could not activate the MTF-1/MRE and Nrf2/ARE pathways, which regulate MT induction. Previously, we investigated the interaction between zinc and cadmium in vascular endothelial cells and found that zinc accumulates within the cells but does not induce MT at the protein level; however, zinc protects cells from cadmium cytotoxicity by decreasing the intracellular accumulation of cadmium (Kaji et al, 1992). In the present study, we confi rmed that ZnSO 4 could not induce MT protein or mRNA.…”

Molecular and other predictors for infertility in patients with varicoceles

“…Specificity of transport is demonstrated by the fact that calcium does not compete with cadmium transport. These data are consistent with in vitro findings that zinc offers protection against cadmium cytotoxicity in cultured vascular endothelial cells by decreasing intracellular cadmium accumulation (333). However, there are still gaps in our knowledge.…”

Bis(1,4-dihydro-2-methyl-1-phenyl-4-thioxo-3-pyridiolato)zinc(II) exhibits strong cytotoxicity and a high intracellular accumulation in cultured vascular endothelial cells

“…The present study demonstrated that Zn-06 could significantly accumulate within various cell types and exhibited cytotoxicity in cells.. Although inorganic zinc is non-toxic to vascular endothelial cells (Kaji et al, 1992), this study showed that zinc in organic forms could be cytotoxic. Moreover, the cytotoxicity can be exhibited in various cell types other than vascular endothelial cells.…”

Zinc diethyldithiocarbamate as an inducer of metallothionein in cultured vascular endothelial cells

“…After a 24-hr treatment with inorganic zinc, MT protein expression was not observed (Fig. 1A), consistent with our previous study (Kaji et al, 1992). Furthermore, ZnSO 4 did not alter the expression of MT-1A, MT-1E, or MT-2A mRNAs, MT subisoforms expressed in bovine tissue (Fig.…”

“…1D), suggesting that the zinc ion could not activate the MTF-1/MRE and Nrf2/ARE pathways, which regulate MT induction. Previously, we investigated the interaction between zinc and cadmium in vascular endothelial cells and found that zinc accumulates within the cells but does not induce MT at the protein level; however, zinc protects cells from cadmium cytotoxicity by decreasing the intracellular accumulation of cadmium (Kaji et al, 1992). In the present study, we confi rmed that ZnSO 4 could not induce MT protein or mRNA.…”

Molecular and other predictors for infertility in patients with varicoceles

“…Specificity of transport is demonstrated by the fact that calcium does not compete with cadmium transport. These data are consistent with in vitro findings that zinc offers protection against cadmium cytotoxicity in cultured vascular endothelial cells by decreasing intracellular cadmium accumulation (333). However, there are still gaps in our knowledge.…”

Bis(1,4-dihydro-2-methyl-1-phenyl-4-thioxo-3-pyridiolato)zinc(II) exhibits strong cytotoxicity and a high intracellular accumulation in cultured vascular endothelial cells

“…The present study demonstrated that Zn-06 could significantly accumulate within various cell types and exhibited cytotoxicity in cells.. Although inorganic zinc is non-toxic to vascular endothelial cells (Kaji et al, 1992), this study showed that zinc in organic forms could be cytotoxic. Moreover, the cytotoxicity can be exhibited in various cell types other than vascular endothelial cells.…”

Zinc diethyldithiocarbamate as an inducer of metallothionein in cultured vascular endothelial cells

“…After a 24-hr treatment with inorganic zinc, MT protein expression was not observed (Fig. 1A), consistent with our previous study (Kaji et al, 1992). Furthermore, ZnSO 4 did not alter the expression of MT-1A, MT-1E, or MT-2A mRNAs, MT subisoforms expressed in bovine tissue (Fig.…”

“…1D), suggesting that the zinc ion could not activate the MTF-1/MRE and Nrf2/ARE pathways, which regulate MT induction. Previously, we investigated the interaction between zinc and cadmium in vascular endothelial cells and found that zinc accumulates within the cells but does not induce MT at the protein level; however, zinc protects cells from cadmium cytotoxicity by decreasing the intracellular accumulation of cadmium (Kaji et al, 1992). In the present study, we confi rmed that ZnSO 4 could not induce MT protein or mRNA.…”

Molecular and other predictors for infertility in patients with varicoceles

“…Specificity of transport is demonstrated by the fact that calcium does not compete with cadmium transport. These data are consistent with in vitro findings that zinc offers protection against cadmium cytotoxicity in cultured vascular endothelial cells by decreasing intracellular cadmium accumulation (333). However, there are still gaps in our knowledge.…”

Bis(1,4-dihydro-2-methyl-1-phenyl-4-thioxo-3-pyridiolato)zinc(II) exhibits strong cytotoxicity and a high intracellular accumulation in cultured vascular endothelial cells

“…The present study demonstrated that Zn-06 could significantly accumulate within various cell types and exhibited cytotoxicity in cells.. Although inorganic zinc is non-toxic to vascular endothelial cells (Kaji et al, 1992), this study showed that zinc in organic forms could be cytotoxic. Moreover, the cytotoxicity can be exhibited in various cell types other than vascular endothelial cells.…”