Possible contribution of DNase γ to immunoglobulin V gene diversification

Okamoto, Noriaki; Okamoto, Mariko; Araki, Shinsuke; Arima, Hiroshi; Mizuta, Ryushin; Kitamura, Daisuke

doi:10.1016/j.imlet.2009.05.005

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…It is important to note that limited enzymatic degradation of DNA at the site of damage is a key element of both DNA repair and DNA recombination (33,34). Some of the DNA-degrading enzymes implicated in DNA repair may have additional roles in the removal of DNA during cell death and, vice versa, some DNases with primary functions in cell death-associated DNA degradation have been proposed to mediate local DNA cleavage in living cells (35,36).…”

Section: Primary Function Of Dna As Carrier Of Genetic Informationmentioning

confidence: 99%

Mechanisms and emerging functions of DNA degradation in the epidermis

Eckhart

Fischer²,

Tschachler³

2012

Front Biosci

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Degradation of DNA is involved in key processes maintaining the integrity of the epidermis such as the cornification of keratinocytes, the removal of damaged cells and the defense against potentially harmful microorganisms. Recent studies have characterized some of the molecular mechanisms and physiological functions of DNA degradation in the epidermis. Deoxyribonuclease (DNase)1L2 and TREX2 were identified as epidermis-specific DNases and DNase 2 was shown to be the predominant DNA-degrading enzyme on the surface of the skin. Here we review the latest insights into the DNA catabolism in the skin and discuss open questions pertaining to the molecular biology of epidermal DNA breakdown.

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Section: Primary Function Of Dna As Carrier Of Genetic Informationmentioning

confidence: 99%

Mechanisms and emerging functions of DNA degradation in the epidermis

Eckhart

Fischer²,

Tschachler³

2012

Front Biosci

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“…Polymerase (RNA) I polypeptide B ( POLR1B ) is a necessary enzyme for transcription. Deoxyribonuclease I-like 3 ( DNASE1L3 ) is involved in immunoglobulin V gene diversification [44]. All aformentioned DETs were examined for down-regulation in the susceptible birds compared with the resistant birds at 5 dpi (Fig.…”

Section: Discussionmentioning

confidence: 99%

Deciphering alternative splicing and nonsense-mediated decay modulate expression in primary lymphoid tissues of birds infected with avian pathogenic E. coli (APEC)

Sun

2017

BMC Genet

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BackgroundAvian pathogenic E. coli (APEC) can lead to a loss in millions of dollars in poultry annually because of mortality and produce contamination. Studies have verified that many immune-related genes undergo changes in alternative splicing (AS), along with nonsense mediated decay (NMD), to regulate the immune system under different conditions. Therefore, the splicing profiles of primary lymphoid tissues with systemic APEC infection need to be comprehensively examined.ResultsGene expression in RNAseq data were obtained for three different immune tissues (bone marrow, thymus, and bursa) from three phenotype birds (non-challenged, resistant, and susceptible birds) at two time points. Alternative 5′ splice sites and exon skipping/inclusion were identified as the major alternative splicing events in avian primary immune organs under systemic APEC infection. In this study, we detected hundreds of differentially-expressed-transcript-containing genes (DETs) between different phenotype birds at 5 days post-infection (dpi). DETs, PSAP and STT3A, with NMD have important functions under systemic APEC infection. DETs, CDC45, CDK1, RAG2, POLR1B, PSAP, and DNASE1L3, from the same transcription start sites (TSS) indicate that cell death, cell cycle, cellular function, and maintenance were predominant in host under systemic APEC.ConclusionsWith the use of RNAseq technology and bioinformatics tools, this study provides a portrait of the AS event and NMD in primary lymphoid tissues, which play critical roles in host homeostasis under systemic APEC infection. According to this study, AS plays a pivotal regulatory role in the immune response in chicken under systemic APEC infection via either NMD or alternative TSSs. This study elucidates the regulatory role of AS for the immune complex under systemic APEC infection.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-017-0488-4) contains supplementary material, which is available to authorized users.

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“…26) Moreover, it is suggested that DNase g is involved in DNA breaks occurring in the gene rearrangement of the immunoglobulin variable (V) region in chicken DT40 B cell line, which also generates somatic hypermutation in immunoglobulin V region. 27) HeLa cells with ectopically expressed DNase g respond to ceramide to promote apoptotic DNA fragmentation, which never is observed in the parental cell line. 28) These results suggest that DNase g could be used in gene therapy to induce apoptotic DNA degradation in solid tumors.…”

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confidence: 97%

Cladribine Enhances Apoptotic Cell Death in Lung Carcinoma Cells Over-Expressing DNase .GAMMA.

Hayakawa

Suzuki

Kamei

et al. 2011

Biological & Pharmaceutical Bulletin

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Worldwide, lung cancer is the most common form of cancer and often has a poor prognosis. Establishment of effective therapies for lung cancer is a major concern in clinical cancer research. We compared the cytotoxic effects of chemotherapeutic agents including cisplatin, 5-fluorouracil, vinorelbine and cladribine, on a human lung cancer cell line, A549, and its derivative transfected with the DNase g g gene. We observed selective cytotoxicity of cladribine on the DNase g g-expressing sub-cell line of A549. Cladribine induces selective apoptosis in DNase g g-expressing A549 cells, which depends on activation of caspases. These results suggest that a combination therapy that includes cladribine along with the introduction of DNase g g has potential as a new therapeutic strategy for lung cancer.

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Possible contribution of DNase γ to immunoglobulin V gene diversification

Cited by 5 publications

References 41 publications

Mechanisms and emerging functions of DNA degradation in the epidermis

Mechanisms and emerging functions of DNA degradation in the epidermis

Deciphering alternative splicing and nonsense-mediated decay modulate expression in primary lymphoid tissues of birds infected with avian pathogenic E. coli (APEC)

Cladribine Enhances Apoptotic Cell Death in Lung Carcinoma Cells Over-Expressing DNase .GAMMA.

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