Positivity to glial fibrillary acidic protein in bone, cartilage, and chordoma

Kasantikul, Vira; Shuangshoti, Samruay

doi:10.1002/jso.2930410109

Cited by 41 publications

(28 citation statements)

References 12 publications

(4 reference statements)

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“…In contrast to cartilaginous cells, expression of GFAP is observed in mature osteocytes from vertebral bodies. 3 Our results, obtained by Q-PCR, which also correlate with the immunohistochemical findings, confirm that GFAP is a bona fide marker for cartilaginous differentiation.…”

Section: Discussionsupporting

confidence: 86%

“…Expression of GFAP in nonglial cells has been described in mature osteocytes and chondrocytes (13/20 osteocyte samples from the vertebral body, 5/ 11 chondrocyte samples from the epiglottis, 3/11 chondrocyte samples from rib cartilage, 2/6 samples from tracheal cartilage, and 4/5 samples from bronchial cartilage), 3 wherein strong cytoplasmatic staining was observed in chondrocytes from bronchial cartilage and tracheal samples stained weakly. 5 These findings suggest that osteocytes and chondrocytes have intermediate filaments in their cytoskeleton that are identical to, are similar to, or associate with GFAP.…”

Section: Discussionmentioning

confidence: 99%

“…5 These findings suggest that osteocytes and chondrocytes have intermediate filaments in their cytoskeleton that are identical to, are similar to, or associate with GFAP. 3 Chondrocytes in elastic cartilage, stain positive for GFAP more frequently than those from nonelastic cartilage (fibrous or hyaline), 8 as do chondrocytes from the epiglottis. These findings suggest a histogenetic relationship between epiglottal cartilage and the neural crest.…”

Section: Discussionmentioning

confidence: 99%

“…2 In addition, GFAP is expressed in osteocytes of mature bones and chondrocytes from epiglottal, bronchial, and tracheal cartilage. [2][3][4][5][6] GFAP is the most specific marker for cells of astrocytic origin under normal and pathological conditions. By immunohistochemistry, however, GFAP has been shown to be expressed in mesenchymal tumors, such as soft tissue myoepitheliomas, peripheral nerve sheath tumors, and chordomas, 3,[7][8][9][10][11][12] and also in mixed tumors, such as salivary gland pleomorphic adenomas.…”

mentioning

confidence: 99%

“…[2][3][4][5][6] GFAP is the most specific marker for cells of astrocytic origin under normal and pathological conditions. By immunohistochemistry, however, GFAP has been shown to be expressed in mesenchymal tumors, such as soft tissue myoepitheliomas, peripheral nerve sheath tumors, and chordomas, 3,[7][8][9][10][11][12] and also in mixed tumors, such as salivary gland pleomorphic adenomas. 13,14 These findings can create problems for the diagnosis of tumors that have similar morphologies and are positive for GFAP, such as myoepithelioma and chondroblastic osteosarcoma of the head and neck.…”

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confidence: 99%

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Glial fibrillary acidic protein in tumor types with cartilaginous differentiation

et al. 2009

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Glial fibrillary acidic protein (GFAP) is a member of the intermediary filament protein family. It is an important component of astrocytes and a known diagnostic marker of glial differentiation. GFAP is expressed in other neural tumors and pleomorphic adenoma and, less frequently, in cartilage tumors, chordomas, and soft tissue myoepitheliomas. The aim of this study was to evaluate the role of GFAP and its reliability in nonglial tumors as an immunohistochemical marker. We evaluated GFAP gene and protein expression using Q-PCR and immunohistochemistry, respectively, in 81 and 387 cases of soft tissue, bone tumors, and salivary pleomorphic adenomas. Immunohistochemistry staining for GFAP was observed in all osteosarcomas (8 cases), all pleomorphic adenomas (7 cases), in 5 of 6 soft tissue myoepitheliomas, and in 21 of 76 chondrosarcomas. By Q-PCR, GFAP was highly expressed in pleomorphic adenomas and, to a lesser extent, chondrosarcomas, soft tissue myoepitheliomas, and chondroblastic osteosarcomas. The results that we obtained by immunohistochemistry and Q-PCR were well correlated. GFAP is a potential marker for tumors with cartilaginous differentiation, supported by evidence that GFAP is expressed in certain cases of myoepithelial tumors by immunohistochemistry, including soft tissue myoepitheliomas, which are related to cartilaginous differentiation. These findings contribute significantly to the diagnosis of soft tissue myoepitheliomas with cartilaginous differentiation and chondroblastic osteosarcoma in mesenchymal tumors.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Glial fibrillary acidic protein in tumor types with cartilaginous differentiation

et al. 2009

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Histochemistry and immunocytochemistry of the developing ependyma and choroid plexus

Sarnat

1998

Microsc. Res. Tech.

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The adult human ependyma expresses no intermediate filament proteins or secretory proteins; the fetal ependyma shows strong immunocytochemical (ICC) expression of vimentin, glial fibrillary acidic protein (GFAP), cytokeratins (CKs) of high molecular weight, glycoproteins, and S‐100β protein. Each has a precise and specific spatial distribution within the developing ependyma and a predictable time of appearance and regression in each region of the ventricular system. Several are coexpressed, but some appear earlier or persist longer than others. Secretory proteins of ependymal cells are important in several developmental processes such as the guidance of axonal growth cones. GFAP is not expressed in the floor plate ependyma at any stage of development, unlike vimentin and CK. The choroid plexus epithelium is a specialized ependyma, with an ICC profile that differs from the surface ependyma: vimentin, CK, and S‐100β protein continue to be expressed throughout fetal and adult life, but GFAP is not expressed. Certain cerebral malformations are associated with specific ICC abnormalities: ependymal S‐100β protein continues to be immunoreactive in disorders of neuroblast migration; ependymal vimentin is focally upregulated in Chiari malformations and congenital aqueductal stenosis. Other mammalian and nonmammalian species have characteristic profiles of ependymal immunoreactivity to the same proteins expressed in humans but exhibit interspecific differences. Microsc. Res. Tech. 41:14–28, 1998. © 1998 Wiley‐Liss, Inc.

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Gliosarcoma with features of chondroblastic osteosarcoma

Hayashi

Ohara

Jeon

et al. 1993

Cancer

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Background. The occurrence of osteoid‐chondral elements in gliosarcoma is extremely rare and has been reported in only two cases. A new, rare case of gliosarcoma with osteosarcomatous differentiation in a 55‐year‐old man and histogenesis of osteoid‐chondral tissue in gliosarcoma is discussed. Methods. Surgically removed tumor tissues were examined immunohistochemically and electron microscopically, and a histologic examination was performed. Results. Immunohistochemical and electron microscopic studies confirmed the presence of glial, fibroblastic, and osteoid‐chondral elements. A major part of the sarcomatous tissue was undifferentiated and stained only by vimentin. Some areas of osteoid‐chondral tissue were positive for glial fibrillary acidic protein (GFAP). Conclusions. These data suggest that osteoid‐chondral elements came from the sarcomatous portion and GFAP positivity is not restricted to astrocytes and has been seen particularly in chondroid areas of non‐glial tumors.

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Positivity to glial fibrillary acidic protein in bone, cartilage, and chordoma

Cited by 41 publications

References 12 publications

Glial fibrillary acidic protein in tumor types with cartilaginous differentiation

Glial fibrillary acidic protein in tumor types with cartilaginous differentiation

Histochemistry and immunocytochemistry of the developing ependyma and choroid plexus

Gliosarcoma with features of chondroblastic osteosarcoma

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