Positively charged supported lipid bilayer formation on gold surfaces for neuronal cell culture

Abstract

Supported lipid bilayers are widely used as cell membrane models and sensor platforms, but the usage on gold surface needs additional surface modification or optimized experimental conditions. In this work, the authors show lipid bilayer formation on plasma activated gold surfaces in physiological conditions without any other modification if at least 30% positively charged lipids are present. Details of bilayer formation from small unilamellar vesicles were monitored using quartz crystal microbalance with dissipation in both basic and acidic environment. The authors also confirmed that this positively charged bilayer system can sustain primary cortical neuron growth and lipid transfer. This method will provide simple means to construct biomimetic interface on gold electrodes.

Impact of Doping a Phytosteryl Sulfate on the Properties of Liposomes Made of Saturated and Unsaturated Phosphatidylcholines

“…The negative values of the ζ-potentials for the three pure lipid samples are in the order POPC＞DPPC＞DOPC, which is in agreement with the previous reports 28,29) . These increase dramatically with the addition of 0.075 mole fraction of PSO 4 .…”

New Frontiers for Selective Biosensing with Biomembrane-Based Organic Transistors

“…However, although this is a straightforward procedure in the case of hydrophilic surfaces ( i.e. , glass), hydrophobic substrates require pretreatments to adjust the surface tension or specific lipid mixtures to promote surface adhesion (Figure A). , To overcome such limitations, solvent-assisted lipid bilayer (SALB) formation might be achieved by rapid solvent exchange from an organic to an aqueous solution, promoting bilayer formation and the possible incorporation of TMPs . Finally, moving toward fully biological membranes, cell blebbing enables the collection of cell membrane vesicles to form a bilayer with proteins that preserves their native conformations and orientations .…”

“…However, on 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) 30% SLB, only dead cells fluoresce, meaning that live cells are able to resist this transfer process, whereas on DOTAP 100% SLB, both live and dead cells fluoresce. Reprinted and adapted with permission from ref . Copyright 2016 American Vacuum Society.…”

Composite Lipid Bilayers from Cell Membrane Extracts and Artificial Mixes as a Cell Culture Platform

“…Yet, the nature of the platform presents not only advantages but also challenges. Although it was shown that some types of cells are able to survive on the unmodified surface of planar solid-supported lipid bilayer (SLB), the biologically inert nature of the lipid headgroups does not provide a starting point for adhesion complex formation. To facilitate cell adhesion, additional functionalization is required, for example, by covalent attachment of components of the ECM , or by surfactant-assisted insertion of transmembrane proteins …”