Positive Predictive Value of Leeds Acinetobacter Medium for Environmental Surveillance of Acinetobacter baumannii

McConnell, Michael J.; Pérez‐Romero, Pilar; Lepe, José Antonio; Pérez-Ordóñez, Ana; Valencia, R; Vázquez-Barba, Isabel; Pachón, Jerónimo

doi:10.1128/jcm.05412-11

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…Each surface was sampled in duplicate using sterile swabs moistened with physiologic saline. One swab from each surface was used to detect A. baumannii using conventional cul-ture methods previously described by our group (6). Briefly, the swab was placed in 1 ml of Luria-Bertani medium and incubated for 24 h at 37°C to enrich bacteria present in the sample.…”

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confidence: 99%

Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment

et al. 2012

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A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria. N osocomial infections caused by drug-resistant bacteria represent an important clinical challenge. Acinetobacter baumannii has become one of the most problematic causative agents of nosocomial infections due to its remarkable ability to survive on hospital surfaces and acquire antibiotic resistance, resulting in the global emergence of multidrug-resistant strains with resistance to multiple antibiotic classes (5). A. baumannii has been especially problematic in critically ill patients in the intensive care setting, as it is an important cause of ventilator-associated pneumonia and bacteremia. In this context, patients are exposed to A. baumannii via contact with contaminated hospital equipment or by contact with hospital personnel carrying the bacteria. A number of studies have demonstrated widespread contamination with A. baumannii on hospital environmental surfaces, most notably in intensive care units (ICUs) (1,4,8,9).Environmental surveillance protocols have been employed for the identification of hospital equipment colonized by A. baumannii so that appropriate decontamination procedures can be carried out (1,4,8,9). Since these surveillance methods employ conventional bacterial culture to determine the presence of A. baumannii, definitive species identification can require between 24 and 48 h. Nucleic acid-based tests, such as real-time PCR, have been employed for the identification of numerous bacterial pathogens (2); however, to our knowledge this technique has not been applied to identifying contaminated hospital equipment. The objective of the present study was to develop a real-time PCR for identifying hospital surfaces colonized by A. baumannii.A real-time PCR assay was developed using TaqMan chemistry for the amplification of nucleotides 774 to 859 of the outer membrane protein A gene (ompA; accession number AY485227). The ompA gene was chosen because it is present in all sequenced genomes of A. baumannii available in the public domain (as of March 2010), and the sequences chosen for the primers and probe correspond to regions highly conserved between published A. baumannii ompA sequences (100% sequence identity). The primers OmpA Forward (5=-TCTTGGTGGTCACTTGAAGC-3=) and Ompa Reverse (5=-ACTCTTGTGGTTGTGGAGCA-3=) and the probe (5=-AAGTTGCTCCAGTTGAACCAACTCCA-3=), 5= labeled with 6-carboxyfluorescein and the 3= labeled with 6-carboxytetramethylrhodamine, were used. A quantification standard, pGEM-ompA, was constructed by inserting the ompA gene from the ATCC 19606 strain...

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Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment

et al. 2012

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“…LAM also contains sucrose and fructose, which have not fermented by Acinetobacter sp., hence resulting in pink coloration of the medium upon growth of Acinetobacter sp. (McConnell et al 2011). …”

Section: Screening Of Cyanophycin Producing Organismsmentioning

confidence: 93%

“…usually produces circular, convex, smooth, opaque colonies with entire margins of 1-2 mm in diameter after 24 h of incubation at 30°C in air (McConnell et al 2011). They possess high growth rate at 30°C and at low pH.…”

Section: Screening Of Cyanophycin Producing Organismsmentioning

confidence: 98%

A Mini Review on Cyanophycin: Production, Analysis and Its Applications

Aravind¹,

Saranya²,

Sudha³

et al. 2016

Integrated Waste Management in India

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Cyanophycin or Cyanophycin Granule Polypeptide (CGP), a non-ribosomally synthesized amino acid polymer, consists of equimolar amounts of arginine and aspartic acid. It can be soluble under acidic (pH < 2) or alkaline (pH > 9) conditions. Cyanophycin has many advantages: high viscosity, high solubility and complete biodegradability. Because of its soluble and polymeric nature, CGP can be used in the fields of medical biotechnology for scaffold production. It has been proposed as an eco-friendly alternative for poly (acrylic acid) as water softening agents. CGP is usually produced by different species of cyanobacteria and also by some heterotrophic bacteria like Acinetobacter sp., Bordetella bronchiseptica, Clostridium botulinum, Desulfitobacterium hafniense, etc. Due to the low yield of CGP by cyanobacteria, bacterial strains are commercially preferred for industrial scale. Acinetobacter have been found to have high accumulation (46 % of cell dry weight) of CGP. Bacterial strains are grown in different production medium and the productivity of cyanophycin was analyzed. It has been found that addition of arginine to the medium has enhanced CGP accumulation. Characterization of cyanophycin can be performed by SDS-PAGE, HPLC and Mass spectroscopy analysis. Native PAGE analysis reveals that CGP occurs in the range of 21-29 kDa. Cyanophycin has potential applications in the fields of biomedicine, agro-chemistry, pharmacy and personal care.

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Métodos microbiológicos para la vigilancia del estado de portador de bacterias multirresistentes

Oteo

Bou

Cháves

et al. 2017

Enfermedades Infecciosas y Microbiología Clínica

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Positive Predictive Value of Leeds Acinetobacter Medium for Environmental Surveillance of Acinetobacter baumannii

Cited by 4 publications

References 7 publications

Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment

Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment

A Mini Review on Cyanophycin: Production, Analysis and Its Applications

Métodos microbiológicos para la vigilancia del estado de portador de bacterias multirresistentes

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