Aim: Postvoiding residual (PVR) urine is measured by urethral catheterization (UC), portable ultrasonography (USG) or voiding cystourethrogram (VCUG) images. We evaluated those three different PVR assessment methods and parameters that may interfere with the results.Patients and Methods: Eighteen patients with spinal bifida (SB) (10 female and 8 male, mean 6.2±0.83 years old) (Group I) Fourteen patients with lower urinary tract symptoms (LUTS) (5 female and 9 male, mean 7.4± 0.6)(Group II). PVR measurements were performed by urethral catheterization (UC), portable Caresono PadScan HD 5 ® USG and on voiding cystourethrogram (VCUG) images. Relation with vesicourethral reflux (VUR), renal scaring (on DMSA), bladder trabeculation (BT) is evaluated.Results: Mean PVR volumes approximately same in the catheter and ultrasound groups (p>0.05). PVR is less in the VCUG grup when compare other groups but statistically not significant (p>0.05). Significant correlation between catheter PVR and USG PVR group (p<0 .01, r: 0.94 and r:0.96) . Although no significant difference is demonstrated for VUR in both groups (p>0.05), PVR values are higher in patients with higher VUR degrees. There was no statistical difference according to existing of scaring in both groups (p>0.05). There was no statistical difference according to existing bladder trabeculation in both groups (p>0.05)Conclusion: Measurement of PVR by portable ultrasonography yields comparable results with urethral catheterization. Although PVR measurement on VCUG images is a less reliable method, it can provide additional information in certain conditions (ie. VUR, diverticulum..). Although statistical significance could not be demonstrated, we believe that VUR, renal scaring and bladder trabeculation may interfere with the results of PVR assessments.