Porphyromonas pogonae sp. nov., an anaerobic but low concentration oxygen adapted coccobacillus isolated from lizards (Pogona vitticeps) or human clinical specimens, and emended description of the genus Porphyromonas Shah and Collins 1988

<i>Porphyromonas bronchialis</i> sp. nov. Isolated from Intraoperative Bronchial Fluids of a Patient with Non-Small Cell Lung Cancer

“…The G + C mol% of a representative isolate PAGU 1600 T was 56.50 ± 0.67 mol%, which were clearly distinct from other non-pigmented Porphyromonas species, i.e., P. catoniae (49 mol%; Willems and Collins 1995), P. bennonis (58 mol%; Summanen et al 2009), and P. pogonae (43.00 ± 0.62 mol%; Kawamura et al 2015).…”

“…Fatty acid analysis revealed that 3OH-iso-C17:0 (18.8-20.4%) predominated, followed by anteiso-C15:0, C16:0, and C18:0 in almost equal amounts (11.1-12.2%, 9.5-10.6%, and 9.6-11.2%, respectively), which could be separated from other non-pigmented Porphyromonas species, i.e., P. catoniae (C15:0 iso, C15:0 antiiso; Willems and Collins 1995;Kawamura et al 2015), P. bennonis (C15:0 DMA, 3OH-C14:0; Summanen et al 2009;Kawamura et al 2015), and P. pogonae (C15:0 iso, C15:0 anteiso, 3OH-C17:0 iso; Kawamura et al 2015) (Table 3). Fermentation end product data showed that propionic acid was the most abundant product (3.94-13.10 mM), followed by acetic acid (1.84-5.26 mM), which differed from other non-pigmented Porphyromonas species, i.e., P. catoniae (propionic and succinic acids, followed by acetic, iso-valeric and lactic acids; Willems and Collins 1995;Kawamura et al 2015), P. bennonis (acetic and succinic acids; Summanen et al 2009;Kawamura et al 2015), and P. pogonae (succinic, propionic and acetic acids, followed by iso-valeric and lactic acids; Kawamura et al 2015) (Table 3).…”

“…Bacterial cell shape was observed by scanning electron microscopy, as described previously (Kawamura et al 2015). Briefly, bacterial colonies were suspended in phosphate-buffered saline, washed three times, and smeared onto a cover glass.…”

“…Numerous bacterial strains of Gram-negative, nonspore-forming, rod-shaped, obligate anaerobic bacteria, belonging to the genus Porphyromonas, have been isolated from oral infections and many other infections throughout the body, e.g., abscesses in the buttock and groin areas, soft tissue infections, amniotic fluid, umbilical cord, pelvic abscesses, vulvovaginitis, and other infected tissues (Shah and Collins 1988;Krieg 2011;Kawamura et al 2015 Taxonomy within the genus Porphyromonas has progressed in these days; however, many strains remain uncharacterized (Fournier et al 2001;Bemis et al 2011).…”

“…Porphyromonas-like strains have been isolated from oral cavities, urogenital system, and intestinal tracts of their hosts (Willems and Collins 1995;Fournier et al 2001;Finegold et al 2004;Hardham et al 2005;Summanen et al 2005Summanen et al , 2009Mikkelsen et al 2008;Bemis et al 2011;Filioussis et al 2015;Kawamura et al 2015). Usually, most of the uncharacterized strains are known to be unavailable between different laboratories.…”