Porphyromonas gingivalis association with inflammatory markers and exosomal miRNA-155 in saliva of periodontitis patients with and without diabetes diagnosed with COVID-19

Bachtiar, Boy M.; Bachtiar, Endang Winiati; Kusumaningrum, Ardiana; Sunarto, Hari; Soeroso, Yuniarti; Sulijaya, Benso; Apriyanti, Efa; Theodorea, Citra Fragrantia; Pratomo, Irandi Putra; Yudhistira,; Efendi, Defi

doi:10.1016/j.sdentj.2022.12.002

Cited by 10 publications

(13 citation statements)

References 36 publications

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“…Given that GCF-IgA appears to correlate with systemic antibody levels against SARS-CoV-2 9 and the simplicity of saliva collection, 5 we suggest that detecting N-antigens in SARS-CoV-2 antibody testing using oral fluids may serve as an early tool to evaluate population immunity and vaccine response, particularly in vulnerable populations (as those with periodontitis) or when blood collection is not feasible.…”

Section: Discussionmentioning

confidence: 95%

“…This study was performed according to the guidelines of the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement. 5 All clinical data and information were obtained from hospital reports (data not shown). The target participants for this study were individuals who had received the BNT162b2 vaccine (either the Pfizer-BioNTech COVID-19 or the Moderna COVID-19 vaccine) at least six months before the study.…”

Section: Methodsmentioning

confidence: 99%

“…The diagnosis of periodontitis (moderate to severe) was determined according to established criteria, as reported in a previous study. 5 …”

Section: Methodsmentioning

confidence: 99%

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Immunoglobulin A response to SARS-CoV-2-N-protein potentially persists in oral fluids of patients with periodontitis six months after mRNA vaccine administration

Bachtiar,

Soeroso,

Haerani

et al. 2024

Journal of Dental Sciences

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Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

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Immunoglobulin A response to SARS-CoV-2-N-protein potentially persists in oral fluids of patients with periodontitis six months after mRNA vaccine administration

Bachtiar,

Soeroso,

Haerani

et al. 2024

Journal of Dental Sciences

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“…Indeed, excessive inflammation seen in patients experiencing a “cytokine storm” is strongly associated with severe disease. 43,47,48…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that oral microbiota could also exert their antiviral effects indirectly through their ability to alter gene expression and hence the susceptibility of target cells. 48,49…”

Section: Discussionmentioning

confidence: 99%

Inhibition of SARS-CoV-2 infection byPorphyromonas gingivalisand the oral microbiome

Bontempo,

Chirino,

Heidari

et al. 2024

Preprint

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The COVID-19 pandemic persists despite the availability of vaccines, and it is therefore crucial to develop new therapeutic and preventive approaches. In this study, we investigated the potential role of the oral microbiome in SARS-CoV-2 infection. Using anin vitroSARS-CoV-2 pseudovirus infection assay, we found a potent inhibitory effect exerted byPorphyromonas gingivalison SARS-CoV-2 infection mediated by knownP. gingivaliscompounds such as phosphoglycerol dihydroceramide (PGDHC) and gingipains as well as by unknown bacterial factors. We found that the gingipain-mediated inhibition of infection is likely due to cytotoxicity, while PGDHC inhibited virus infection by an unknown mechanism. Unidentified factors present inP. gingivalissupernatant inhibited SARS-CoV-2 likely via the fusion step of the virus life cycle. We addressed the role of other oral bacteria and found certain periodontal pathogens capable of inhibiting SARS-CoV-2 pseudovirus infection by inducing cytotoxicity on target cells. In the human oral cavity, we observed the modulatory activity of oral microbial communities varied among individuals in that some saliva-based cultures were capable of inhibiting while others were enhancing infection. These findings contribute to our understanding of the complex relationship between the oral microbiome and viral infections, offering potential avenues for innovative therapeutic strategies in combating COVID-19.

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Effectiveness of 0.2% Hyaluronic Acid on Clinical, Biomolecular and Microbiological Parameters in Type 2 Diabetes Mellitus Patients with Periodontitis

Devina,

Halim,

Meivi

et al. 2024

Eur J Dent

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Objective This double-blind randomized clinical trial assessed the effectiveness of 0.2% hyaluronic acid (HA) gel as an adjunct to scaling and root planning (SRP) in patients with periodontitis and type 2 diabetes mellitus (DM), focusing on changes in clinical periodontal parameters, the expression of inflammatory mediators, and oral pathogens. Materials and Methods The randomized clinical trial involved 36 participants, 18 DM patients, and 18 healthy patients. The participants in each group were randomly assigned to receive placebo or HA gel after SRP. Gingival crevicular fluid and subgingival plaque samples were taken before treatment and at 4-week follow-up. Clinical parameters, interleukin-1β (IL-1β) and IL-10 levels, and proportions of Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn) were evaluated at baseline and follow-up. Statistical Analysis Paired t-test (parametric data) or Wilcoxon signed-rank test (nonparametric data) was used for intragroup comparison between baseline and follow-up, and comparisons between groups one-way analysis of variance test (parametric data) or Kruskal–Wallis test (nonparametric data). Results At 4 weeks, most of the groups showed statistically significant decreases (p ≤ 0.05) in various clinical and biomolecular parameters. However, there were exceptions: the pocket probing depth (PPD) and clinical attachment loss (CAL) parameter did not significantly decrease for the placebo (p > 0.05) non-DM group, and the IL-10 parameter in the DM HA gel group (p = 0.108). Regarding bacterial proportions, the non-DM and DM placebo group exhibited significant test results for Pg after 4 weeks (p ≤ 0.05). In the case of Fn bacteria proportions, they decreased in all groups, but these results were not statistically significant (p ≥ 0.05). An intergroup analysis revealed no significant differences (p ≤ 0.05) for bleeding on probing (BOP), PPD, and both proinflammatory and anti-inflammatory cytokines. Only clinical attachment loss (CAL) exhibited a statistically significant intergroup difference 0.042. Conclusion The use of 0.2% HA gel into periodontal pockets alongside SRP, for both diabetic and healthy individuals, showed no statistically significant variances in clinical, biomolecular, and microbiological measures.

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Porphyromonas gingivalis association with inflammatory markers and exosomal miRNA-155 in saliva of periodontitis patients with and without diabetes diagnosed with COVID-19

Cited by 10 publications

References 36 publications

Immunoglobulin A response to SARS-CoV-2-N-protein potentially persists in oral fluids of patients with periodontitis six months after mRNA vaccine administration

Immunoglobulin A response to SARS-CoV-2-N-protein potentially persists in oral fluids of patients with periodontitis six months after mRNA vaccine administration

Inhibition of SARS-CoV-2 infection byPorphyromonas gingivalisand the oral microbiome

Effectiveness of 0.2% Hyaluronic Acid on Clinical, Biomolecular and Microbiological Parameters in Type 2 Diabetes Mellitus Patients with Periodontitis

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