Population structure and genetic diversity in bottle gourd [Lagenaria siceraria (Mol.) Standl.] germplasm from India assessed by ISSR markers

Bhawna,; Abdin, M. Z.; Arya, Lalit; Saha, Dipnarayan; Sureja, Amish K; Pandey, Chitra Devi; Verma, Manjusha

doi:10.1007/s00606-014-1000-5

Cited by 35 publications

(17 citation statements)

References 10 publications

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“…This could be attributed to higher cross pollination of bottle gourd or long-term selection by farmers. Furthermore, bottle gourd is a cross-pollinated crop, where recurrent exchange of gametes occurs between different genotypes (Bhawna et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Mashilo

Shimelis²,

Odindo³

et al. 2016

horts

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Bottle gourd [Lagenaria siceraria (Molina) Standl.] landraces are widely grown in South Africa, and genetic diversity analysis is necessary to identify promising genotypes for breeding or systematic conservation. Sixty-seven diverse bottle gourd landraces were genotyped using 14 selected simple sequence repeat (SSR) markers. The number of alleles detected per marker ranged from 4 to 11, with a total of 86 putative alleles being amplified. Allele sizes ranged from 145 to 330 base pair (bp). Number of effective alleles (Ne) ranged from 1.58 to 6.14 with a mean of 3.10. Allelic richness varied from 3.00 to 8.90 with a mean of 5.23. Expected heterozygosity (He) values ranged from 0.37 to 0.84 with a mean of 0.65. The mean polymorphic information content (PIC) was 0.57. Jaccard’s coefficient of similarity values ranged from 0.00 to 1.00, with a mean of 0.63. Analysis of molecular variance (AMOVA) revealed that 79%, 17%, and 4% of the variation in bottle gourd landraces was attributable to among landraces, within landraces, and between populations, respectively. The study established the existence of considerable genetic diversity among South African bottle gourd landraces. Unique landraces such as BG-4, BG-6, BG-8, BG-9, and BG-15 from cluster I; BG-55, BG-42, BG-57, and BG-58 from cluster II; BG-28, BG-23, BG-29, and BG-34 from cluster III were selected based on their highest dissimilarity index. These could be useful for bottle gourd breeding and systematic conservation.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Mashilo

Shimelis²,

Odindo³

et al. 2016

horts

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“…For instance, the genetic differentiation between Capricorn and Waterberg districts was 0.014 probably due to the high level of gene flow (17.43). The high level of gene flow observed may be attributed to an exchange of seed materials (Bhawna et al, 2014) between farmers in the two districts leading to low levels of genetic differentiation (Bhawna et al, 2015). This was supported by a low value of genetic distance (0.002) and high genetic identity (0.973).…”

Section: Discussionmentioning

confidence: 88%

Simple Sequence Repeat Markers Reveal Genetic Diversity Within and among Landrace Collections of Citron and Dessert Watermelon from South Africa

Mashilo¹,

Shimelis²,

Odindo³

et al. 2016

J. Amer. Soc. Hort. Sci.

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Genetic diversity analysis is fundamental for effective breeding and genetic conservation. The objective of this study was to determine the genetic diversity present among dessert watermelon (Citrullus lanatus var. lanatus) and citron watermelon (C. lanatus var. citroides) landraces widely grown in South Africa and to select genetically diverse and complimentary genotypes for strategic breeding or conservation. Thirty-one dessert watermelon and 34 citron watermelon landraces were genotyped using 10 polymorphic simple sequence repeat markers. The number of alleles detected per marker ranged from 2 to 23 alleles, with a mean of 13.5 alleles. A total of 135 putative alleles were amplified from sampled watermelon populations. Number of effective alleles ranged from 1.99 to 10.88 alleles with a mean of 5.83 alleles. The mean observed and expected heterozygosity were 0.50 and 0.79, respectively. The mean polymorphic information content was 0.79. Cluster and principal coordinate analyses grouped the two watermelon populations into two separate clusters. The two populations were genetically differentiated with low gene flow, suggesting the presence of high genetic differences between the two populations. Overall, the study established the existence of considerable genetic diversity among South African grown dessert and citron watermelon landraces. Unique dessert watermelon landraces such as SWM-39, SWM-24, SWM-01, SWM-40, SWM-18, SWM-36, and SWM-26; and citron watermelon genotypes including WWM-24, WWM-37, WWM-28, WWM-34, WWM-02, WWM-22, WWM-50, and WWM-36 were selected based on their high dissimilarity index. These could be useful for breeding and systematic conservation.

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“…The ten primers used were: BECKY (CA)7-YC P = 50; CHRYS (CG)7-YG P = 50; TERRY (GTC)4-RC P = 70; UBC 810 (GA)8-C P = 100; UBC 812 (GA) 8-A P = 100; UBC 820 GT)8-C P = 52; UBC 830 (TC)8-G P = 75; UBC 864 (CT) 7-VDV P = 52 e UBC 890 (GT)-VHV P = 90, respectively. The criterion of the primers is according to Manica-Cattani et al (2009) that obtained 65% of polymorfic bands for the Lippia alba for 17 primers ISSR, being considered reproducible and of high quality (Bhawna et al, 2014). These primers were generated 253 polymorphic bands in the analyzed individuals ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity of <i>Lippia rotundifolia</i> Cham. in Minas Gerais, Brazil

Meira¹,

Martins²,

Resende³

2019

Acta Sci. Biol. Sci.

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Lippia rotundifolia is an aromatic species, endemic from Campos rupestres and isolated by mountains. We aimed with the research to study the genetic variability of chá-de-pedestre (Lippia rotundifolia Cham.) with natural occurrence in ten places of the State of Minas Gerais, from molecular markers of ISSR type. The places of collection were: Parque Estadual de Serra Nova; Parque Estadual Veredas do Peruaçu; Abóboras community; Gigante community; edge of Rio do Peixe; environmental preservation area of Olhos d´água; private property in Joaquim Felício; Parque Estadual do Rio Preto; São Gonçalo do Rio das Pedras and brook of Rio Tigre. After the establishment of the extraction and amplification of DNA fragments with primers ISSR, an array of genetic distance was built. From this matrix, we analyzed the genetic diversity index as the allelic frequency (Na and Ne), Shannos´s index (H´), polymorphism (PLP), heterozygosity (He) and gene flow (Nm). The result showed higher genetic variability within the population (93%). The genetic diversity index was low (He = 0.132; H´= 0.214; Na = 1.111; Ne = 1.183; PLP = 56.67%). In general the species have low genetic diversity. The greatest diversity in populations occurred with an average temperature of 20°C. The Rio Tigre showed higher genetic distance and isolation.

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Population structure and genetic diversity in bottle gourd [Lagenaria siceraria (Mol.) Standl.] germplasm from India assessed by ISSR markers

Cited by 35 publications

References 10 publications

Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Simple Sequence Repeat Markers Reveal Genetic Diversity Within and among Landrace Collections of Citron and Dessert Watermelon from South Africa

Genetic diversity of <i>Lippia rotundifolia</i> Cham. in Minas Gerais, Brazil

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