Population genetic analysis of insertion–deletion polymorphisms in a Brazilian population using the Investigator DIPplex kit

Study of InDel genetic markers with forensic and ancestry informative interest in PALOP’s immigrant populations in Lisboa

“…Furthermore, InDels combine characteristics of STRs and SNPs, filling some gaps of each one of these markers and introducing a new class of genetic markers for forensic purposes. Advances in forensic genetics are noticed over the past few years and show us the interest for the development of biallelic markers, such as SNPs and InDels [9,16].…”

“…This kit allows the study of 30 InDel markers and the homologous gene of amelogenin as informative of the individual's sexual gender [12,16,17]. This multiplex has the advantage that amplified products can be separated and analyzed by capillary electrophoresis using an automatic sequencer, with similar technology to STR analysis.…”

“…The Investigator® DIPplex kit (Qiagen, Hilden, Germany) has already been employed in some population studies, specially in populations of European, American and Asian origin [8][9][10]12,[16][17][18][19][20][21][22][23][24]. Demonstrating high discrimination power and high power of exclusion, has shown to be proper for forensic cases, specially, in individual identification that require analysis of degraded samples.…”

Evaluation of 30 insertion/deletion polymorphisms as forensic markers in the Kuwaiti population

“…To assess how genetically distant the Kuwaiti population is from other global populations, PCA was conducted based on allele frequencies obtained from published data for 26 different populations: Kuwait (N=150, this study), Iraq (N=101) [4], Turkey (N=93) [4], Lithuania (N=110) [4], Slovenia (N=157) [4], Iran (N=253) [5], Spain (N=71) [6], Poland (N=122) [7], Italy (N=200) [8], Finland (N=151) [9], Greece (N=177) [10], Portugal (N=108) [11], Japan (N=251) [12], Spain (Basque Country) (N=60) [6], Czech Republic (N=55) [13], Somalia (N=175) [9], Taiwan (N=126) [7], Uruguay (N=131) [14], Korea (N=373) [3], Brazil (N=519) [15], Germany (N=80) [16], Han Chinese (N=565) [2], US Caucasian (N=262) [17], US Asian (N=50) [17], US Hispanic (N=140) [17] and US African (N=260) [17]. The final data set consisted of 4,707 individuals typed for the DIPplex loci.…”

A GHEP-ISFG collaborative study on the genetic variation of 38 autosomal indels for human identification in different continental populations

“…When comparing the studied 38 indel set with the 30 indel set commercially available in the Investigator ® DIPplex Kit (Qiagen, Hilden, Germany), accumulated PD values were about two orders of magnitude higher in population samples from Spain, Iraq, Brazil, and Uruguay [33][34][35][36], and three orders of magnitude higher in populations from México, South Africa, and East Asia [17,[37][38][39]. These results support previous findings showing higher forensic information content of the 38 indel panel compared to the Investigator ® DIPplex Kit in US African American, Caucasian, East Asian, and Hispanic samples [40] (see Supplementary Table 8 for details).…”