Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov., isolated from the desert

Abstract

Two strains of bacteria, JC213 T and JC215 T , were isolated from desert soil. Colonies were red to pink and cells Gram-stain-negative. Both strains were oxidase-and catalase-positive and hydrolysed casein. In both strains, phosphatidylethanolamine was the major polar lipid, iso-C 15 : 0 was the major fatty acid and the bacteriohopane derivative, BHD1, was the major hopanoid. The genomic DNA G+C contents of strains JC213 T and JC215 T were 52.7 and 46.3 mol%, respectively. 16S rRNA gene sequence comparisons indicated that both strains belong to the genus Pontibacter within the family Cytophagaceae and the phylum Bacteroidetes. Strain JC213 T showed the highest sequence similarity to Pontibacter populi HLY7-15 T (96.6 %) and with other species of the genus Pontibacter sequence similarity was less than 96 %. Strain JC215 T exhibited highest sequence similarity with Pontibacter lucknowensis DM9 T (95.1 %) and shared 95 % or less sequence similarity with other species of the genus Pontibacter. The sequence similarity between strains JC213 T and JC215 T was 95.8 %. Distinct morphological, physiological and genotypic differences from previously described taxa support JC213 T and JC215 T being representatives of two novel species of the genus Pontibacter, for which the names Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov. are proposed and the type strains are JC213 T (5KCTC 32442 T 5LMG 27669 T ) and JC215 T (5KCTC 32443 T 5LMG 27670 T ), respectively.

Pontibacter ummariensis sp. nov., isolated from a hexachlorocyclohexane-contaminated soil

“…rhizosphera' (Raichand et al, 2011) are not yet validly published (http://www.bacterio.net/pontibacter.html). Type strains of this genus are widely distributed and have been isolated mainly from soil samples, collected from deserts (Zhou et al, 2007;Cao et al, 2014;Subhash et al, 2014), pond sediment (Singh et al, 2013, solar salterns (Joung et al, 2011Subhash et al, 2013), forests (Dastager et al, 2010;Xu et al, 2012), mountains (Srinivasan et al, 2014), the plant rhizosphere (Raichand et al, 2011;Dai et al, 2014) and hexachlorocyclohexane (HCH) dumpsites (Dwivedi et al, 2013;Singh et al, 2014). Apart from soil samples, strains have also been isolated from a marine coelenterate (Nedashkovskaya et al, 2005), desert sand (Zhang et al, 2008;Xu et al, 2014), sea water and muddy water (Suresh et al, 2006;Wang et al, 2010).…”

Pontibacter amylolyticus sp. nov., isolated from a deep-sea sediment hydrothermal vent field

“…At the time of writing, the genus Pontibacter comprised 20 species with validly published names. Members of the genus Pontibacter have been isolated from an actinian (Nedashkovskaya et al, 2005), seawater (Kang et al, 2013), a solar saltern (Subhash et al, 2013), a drainage system (Suresh et al, 2006), soils (Dastager et al, 2010;Wang et al, 2010;Xu et al, 2012;Cao et al, 2014;Dai et al, 2014;Srinivasan et al, 2014), hexachlorocyclohexanecontaminated soils (Dwivedi et al, 2013;Singh et al, 2013Singh et al, , 2014Singh et al, , 2015, and desert soils or sands Zhang et al, 2008Zhang et al, , 2013Subhash et al, 2014;Xu et al, 2014). Three additional species, 'Pontibacter salisaro' (Joung et al, 2011), 'Pontibacter rhizosphera' (Raichand et al, 2011) and 'Pontibacter jeungdoensis' (Joung et al, 2013) have been described; however, the names had not validly published as of September 2015.…”

“…The major pigment constituents of strain 9-2 T were represented by peak 1 (retention time 14.55 min) and peak 3 (retention time 16.68 min) (data not shown). The HPLC elution profile indicated absence of astaxanthin in strain 9-2 T , while some members of the genus Pontibacter, such as Pontibacter odishensis JC130 T , Pontibacter ruber JC213 T and Pontibacter deserti JC215 T , were reported to contain astaxanthin (Subhash et al, 2013(Subhash et al, , 2014.…”

“…Pontibacter ruber JC213 T and Pontibacter deserti JC215 T , were reported to contain astaxanthin (Subhash et al, 2013(Subhash et al, , 2014.…”

Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment

“…The species belonging to the genus Pontibacter are Gram-stain-negative, heterotrophic, aerobic, rod-shaped, motile by means of gliding or nonmotile, have MK-7 (menaquinone 7) as the predominant respiratory quinone and phosphatidylethanolamine as the major polar lipid. At the time of writing, 24 species of the genus Pontibacter have been isolated and classified from diverse niches such as desert soils, muddy water, seawater, hexachlorocyclohexane (HCH)-contaminated soil and pond sediments, forest soils, solar salterns, mountain soils, rhizosphere soils and also from marine actinians (Nedashkovskaya et al, 2005;Suresh et al, 2006;Zhou et al, 2007;Zhang et al, 2008;Dastager et al, 2010;Raichand et al, 2011;Xu et al, 2012;Dwivedi et al, 2013;Kang et al, 2013;Subhash et al, 2013Subhash et al, , 2014Singh et al, 2014Singh et al, , 2015Srinivasan et al, 2014;Mahato et al, 2015). However, out of the 24 known species of the genus Pontibacter, the names of three species, namely 'Pontibacter salisaro' (Joung et al, 2011), 'Pontibacter jeungdoensis' and 'Pontibacter rhizosphera' (Raichand et al, 2011), have yet to be validly published according to the List of Prokaryotic names with Standing in Nomenclature (http://www.…”