Pontibacter niistensis sp. nov., isolated from forest soil

Abstract

A Gram-negative, rod-shaped bacterial strain, NII-0905 T , that was motile by gliding was isolated from soil of a dense forest collected from the Western Ghats of India and its taxonomic position was established. Strain NII-0905 T contained MK-7 as the major menaquinone and anteiso-C 17 : 0 , anteiso-C 15 : 0 , iso-C 16 : 0 and iso-C 15 : 0 as the major cellular fatty acids. The DNA G+C content of strain NII-0905 T was 51.47 mol%. 16S rRNA gene sequence-based phylogenetic analysis confirmed the placement of strain NII-0905 T in the genus Pontibacter and strain NII-0905 T exhibited 93.9-96.3 % 16S rRNA sequence similarity with type strains of species of the genus Pontibacter. On the basis of genotypic and phenotypic evidence, strain NII-0905 T is considered to represent a novel species of the genus Pontibacter, for which the name Pontibacter niistensis sp. nov. is proposed. The type strain is NII-0905 T (5NCIM 5339 T 5CCTCC AA 209057 T ).

Pontibacter diazotrophicus sp. nov., a Novel Nitrogen-Fixing Bacterium of the Family Cytophagaceae

“…niistensis and P . populi from forest soil [29], [30], P . rhizosphera from the rhizosphere soil of Nerium indicum [31], P .…”

Pontibacter chinhatensis sp. nov., isolated from pond sediment containing discarded hexachlorocyclohexane isomer waste

“…β-Galactosidase activity was detected in strain LP51 T , but this activity was absent from P. niistensis NII-0905 T . Hydrolysis of gelatin was observed in strain LP51 T but not P. niistensis NII-0905 T , while hydrolysis of Tween 80 and nitrate reduction were observed in P. niistensis NII-0905 T but not in strain LP51 T (Dastager et al , 2010).…”

“…Species of the genus Pontibacter are distributed widely in nature, and have been isolated from marine actinians, desert soil, muddy water, sea water, forest soil, hexachlorocyclohexane- (HCH) contaminated soil and solar salterns (Nedashkovskaya et al , 2005; Suresh et al , 2006; Zhou et al , 2007; Dastager et al , 2010; Joung et al , 2011, 2013; Dwivedi et al , 2013; Kang et al , 2013; Singh et al , 2013; Zhang et al , 2013; Cao et al , 2014; Subhash et al , 2014). At the time of writing, the genus Pontibacter encompassed 21 species with effectively published names, the names of three of which have not been validly published: Pontibacter actiniarum (the type species; Nedashkovskaya et al , 2005), P. akesuensis (Zhou et al , 2007), P. korlensis (Zhang et al , 2008), P. niistensis (Dastager et al , 2010), P. roseus (Suresh et al , 2006; Wang et al , 2010), P. xinjiangensis (Wang et al , 2010), ‘P. salisaro ’ (Joung et al , 2011), P. populi (Xu et al , 2012), P. lucknowensis (Dwivedi et al , 2013), P. saemangeumensis (Kang et al , 2013), P. ramchanderi (Singh et al , 2013), P. toksunensis (Zhang et al , 2013), P. odishensis (Subhash et al , 2013), ‘P.…”

“…All tests were performed with the single reference strain P. korlensis X14-1 T after taking the opinion of experts in the field. This is because efforts were made to obtain the type strain of P. niistensis from the two culture collections where it was deposited (the NCIM in Pune, India, and the CCTCC in Wuhan, China) and from the authors who described the species (Dastager et al , 2010). The authors were unable to revive the strain, and the two culture collection centres confirmed that the strain was not available.…”

“…Biochemical differences between strain LP51 T and P. niistensis NII-0905 T as originally described include their differential ability to utilize different carbon sources and to grow at different ranges of pH, salinity and temperature. Growth of strain LP51 T occurred at 5–45 °C, 0–9 % (w/v) NaCl and pH 5.0–10, while P. niistensis NII-0905 T was reported to grow at 15–42 °C, 0–10 % NaCl and pH 5–12 (Dastager et al , 2010). P. niistensis NII-0905 T assimilated starch, arabinose and mannitol, while strain LP51 T assimilated inositol, trehalose, rhamnose and xylitol.…”