Ponicidin and Oridonin Are Responsible for the Antiangiogenic Activity of Rabdosia rubescens, a Constituent of the Herbal Supplement PC SPES

Meade-Tollin, Linda C.; Wijeratne, E. M. Kithsiri; Cooper, D.B.; Guild, Mischa; Jon, Edlyn; Fritz, Anne; Zhou, Guang Xiong; Whitesell, Luke; Liang, Jing; Gunatilaka, A. A. Leslie

doi:10.1021/np0304114

Cited by 65 publications

(47 citation statements)

References 16 publications

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“…It exhibited an even pseudomolecular ion peak at m/z 514 [M+Na] + in the ESIMS spectrum, suggesting that it might be a N-containing compound. The HRESIMS data (m/z 514.2430 [M+Na] + , calcd for 514.2416) further confirmed this assumption, giving rise to the molecular formula C 26 H 37 NO 8 . Its strong IR absorptions at 3387 and 1711 cm À1 suggested the presence of hydroxyl and carbonyl groups.…”

Section: Resultsmentioning

confidence: 59%

“…7 Recently, pharmaceutical study showed that oridonin and ponicidin had significant antiangiogenic activity. 8 More recently, these two compounds were found to be potent inhibitors of NF-kB transcription activity and the expression of its downstream targets, COX-2 and inducible nitric-oxide synthase. 9 With an aim to isolate more potent and selective NF-kB inhibitors, we further systematically reinvestigated the chemical constituents of I. rubescens, and 31 7,20-epoxy-ent-kauranoids, including 12 new ones, hebeirubescensins A-L (1-12), were isolated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytotoxic ent-kauranoid derivatives from Isodon rubescens

Huang

Zhou

et al. 2006

Tetrahedron

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Section: Resultsmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Cytotoxic ent-kauranoid derivatives from Isodon rubescens

Huang

Zhou

et al. 2006

Tetrahedron

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“…Research showed that oridonin was one of the most important antitumor active ingredient of Rabdosia rubescens (33)(34)(35)(36). Our previous studies showed that oridonin could arrest the cell cycle in G 2 /M phase in human gastric cancer SGC-7901 cells (3).…”

Section: Discussionmentioning

confidence: 99%

Oridonin induces apoptosis through the mitochondrial pathway in human gastric cancer SGC-7901 cells

Gao

Tan

Zhu

et al. 2016

International Journal of Oncology

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Abstract.Oridonin is one of the most important antitumor active ingredients of Rabdosia rubescens. Recently published studies from our laboratory have demonstrated that oridonin was able to arrest human gastric cancer SGC-7901 cells at G 2 /M phase. However, little is known about inducing apoptosis in gastric cancer. The aim of this study was to investigate the effect of oridonin on antineoplastic capability of SGC-7901 cells and the detailed molecular mechanism of oridoninmediated intrinsic pathway of apoptosis. Cell proliferation was assessed by MTT assay while apoptosis induced by oridonin was determined by Hoechst 33342 staining assay and Annexin V/PI double staining assay. Early apoptotic rate was stained by Annexin V/PI and detected by flow cytometry. Apoptosis pathway was analyzed by western blot analysis of Bcl-2, Bax, cytochrome c and caspase-3 expression. The results showed that oridonin was able to inhibit the SGC-7901 cell proliferation, the 50% growth inhibition (IC 50 ) was 22.74 µM. Oridonin could induce cell apoptosis of SGC-7901 cells and the early apoptotic rates induced by 0, 20, 40, 80 µmol/l oridonin were 1.53±0.67, 3.33±0.29, 84.80±0.82 and 96.43±0.51%, respectively. Western blot analysis revealed that oridonin downregulated Bcl-2 protein (the anti-apoptotic factor) and upregulated Bax protein (pro-apoptotic factor), eventually leading to a reduction in the ratio of Bcl-2/Bax proteins. Furthermore, oridonin induced the release of cytochrome c from the mitochondria to the cytosol and the activation of caspase-3. Taken together, the current study suggested that oridonin induced apoptosis in SGC-7901 cells via the mitochondrial signal pathway, which may represent one of the major mechanisms of oridonin-mediated apoptosis in SGC-7901 cells.

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“…Thus baicalin, the main agent of Scutellaria baicalensis, has been demonstrated to inhibit proliferation in prostate and myeloblastic/promyelocytic leukaemia cell lines (15) or breast cancer cell lines (16), respectively. Rabdosia rubescens inhibits growth of breast cancer cells as well as angiogenesis (17), the active agents have been identified as ponicidin and oridonin (18).…”

Section: Discussionmentioning

confidence: 99%

The effects of PC-Spes on chemosensitive and chemoresistant head and neck cancer cells and primary mucosal keratinocytes

Schmidt

Polednik²,

Gruensfelder³

et al. 2009

Oncol Rep

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Abstract. PC-Spes is a preparation of eight Chinese herbs, which exhibits antiproliferative and antitumour activity in diverse cancer types in vivo and in vitro. We exposed the head and neck squamous carcinoma cell lines (HNSCC) FADU, HLaC79 and HLaC79-clone1, which is a paclitaxelresistant descendant of HLaC79, as well as primary cultured mucosal keratinocytes to increasing concentrations of paclitaxel and/or PC-Spes. Growth inhibition was measured using the MTT assay. While FADU and HLaC79 were growth inhibited by paclitaxel, HLaC79-clone1 cells proved to be resistant against paclitaxel up to doses of 100 nM, whereas all three cell lines were growth inhibited by PC-Spes. Interestingly primary keratinocytes were less sensitive to PC-Spes, they even showed better survivel at low PC-Spes doses. Furthermore, we analyzed cell cycle distribution, apoptosis and tubulin expression level and polymerization status in the HNSCC cell lines. PC-Spes caused a slight decrease of cells in S/G2 phase in HLaC79-clone1. In FADU and HLaC79 cells the cell cycle was shifted towards S/G2 phase as expected. Apoptosis was initiated in all three cell lines by PC-Spes, in mucosal keratinocytes, however, it was triggered less distinctively. In summary, PC-Spes revealed distinct growth inhibition in a paclitaxel-resistant cell line, whereas primary mucosal keratinocytes were less sensitive. PC-Spes might therefore provide a therapeutical approach in chemoresistant head and neck cancer.

show abstract

Ponicidin and Oridonin Are Responsible for the Antiangiogenic Activity of Rabdosia rubescens, a Constituent of the Herbal Supplement PC SPES

Cited by 65 publications

References 16 publications

Cytotoxic ent-kauranoid derivatives from Isodon rubescens

Cytotoxic ent-kauranoid derivatives from Isodon rubescens

Oridonin induces apoptosis through the mitochondrial pathway in human gastric cancer SGC-7901 cells

The effects of PC-Spes on chemosensitive and chemoresistant head and neck cancer cells and primary mucosal keratinocytes

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