Pomegranate (Punica Granatum L.) Breeding

Holland, Doron; Bar-Ya’akov, Irit

doi:10.1007/978-3-319-91944-7_15

Cited by 16 publications

(7 citation statements)

References 88 publications

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“…PuA is primarily derived from pomegranate seeds. As pomegranate requires a long hot and dry season to thrive, it grows only in the subtropical to tropical climates of the Mediterranean Basin, Asia, America, South Africa, and Australia [14]. With the recent emergence of health benefits associated with PuA, the demand for PSO and pomegranates, in general, has surged over the past decade.…”

Section: Introductionmentioning

confidence: 99%

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Yarrowia lipolytica as a Platform for Punicic Acid Production

Urbanikova,

Park,

Krajciova

et al. 2023

IJMS

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Punicic acid (PuA) is a polyunsaturated fatty acid with significant medical, biological, and nutraceutical properties. The primary source of punicic acid is the pomegranate seed oil obtained from fruits of trees that are mainly cultivated in subtropical and tropical climates. To establish sustainable production of PuA, various recombinant microorganisms and plants have been explored as platforms with limited efficiencies. In this study, the oleaginous yeast Yarrowia lipolytica was employed as a host for PuA production. First, growth and lipid accumulation of Y. lipolytica were evaluated in medium supplemented with pomegranate seed oil, resulting in the accumulation of lipids up to 31.2%, consisting of 22% PuA esterified in the fraction of glycerolipids. In addition, lipid-engineered Y. lipolytica strains, transformed with the bifunctional fatty acid conjugase/desaturase from Punica granatum (PgFADX), showed the ability to accumulate PuA de novo. PuA was detected in both polar and neutral lipid fractions, especially in phosphatidylcholine and triacylglycerols. Promoter optimization for PgFADX expression resulted in improved accumulation of PuA from 0.9 to 1.8 mg/g of dry cell weight. The best-producing strain expressing PgFADX under the control of a strong erythritol-inducible promoter produced 36.6 mg/L PuA. These results demonstrate that the yeast Y. lipolytica is a promising host for PuA production.

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Section: Introductionmentioning

confidence: 99%

“…Sci. 2023, 24, x FOR PEER REVIEW 2 of 17 [14]. With the recent emergence of health benefits associated with PuA, the demand for PSO and pomegranates, in general, has surged over the past decade.…”

Section: Introductionmentioning

confidence: 99%

Yarrowia lipolytica as a Platform for Punicic Acid Production

Urbanikova,

Park,

Krajciova

et al. 2023

IJMS

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“…Recent researches were devoted to the transmission of traits from generation to generation, genetic variability, genome, transcriptome and metabolome studies. Recent researches were also carried to develop new resistant ones 4 .…”

Section: Introductionmentioning

confidence: 99%

Optimization of Development of Pomegranate (Punica granatum L.) Varieties from Microclonal Propagation

Ubaydullae¹,

Bolkiev²,

Abdullaev³

et al. 2023

Asian J. of Plant Sciences

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Background and Objective: Besides, increasing cost-efficiency of MS and WPM nutrient media for the regeneration of explants and rhizogenesis of microshoots was planned by introducing changes in the composition of phytohormones. Studying the effect of a supramolecular complex of glycyrrhizinic and salicylic acids on the rhizogenesis of microshoots was aimed in terms of economic benefits. The research was aimed at optimizing in vitro microclonal propagation of some pomegranate varieties (Punica granatum L.).Materials and Methods: August, Kazake-anar, Achikh-dona and Tuyatish varieties of local pomegranate were selected in the study.Various concentrations of sterilants were used for the surface sterilization of explants. Explants were grown in MS and WPM media containing various concentrations of cytokinin, auxin and gibberellic acid. The supramolecular complex was used for microshoot rhizogenesis. Moreover, optimal conditions for acclimatization in microplants were developed. Results: The addition of gibberellic acid into MS and WPM nutrition media did not result in significant differences in the microshoot length. But the addition speeded up the formation of buds. Only earlier development of buds in some varieties were observed. The supramolecular complex used in this work at 0.15 and 0.17 mg LG 1 led to several-fold improvement in root number and length. No significant changes were observed between MS and WPM culture media. Economically utilizing the supramolecular complex was about 45 times cheaper than the mixture of IBA and IAA that was added to MS and WPM nutrition media. Conclusion: An enhanced cost-efficiency of the technology that enables obtaining pomegranate microshoot during the whole year.

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“…Pomegranates have more than 500 globally distributed varieties, of which 50 are commercially cultivated [ 8 ]. Pomegranate has a medium juvenile period of 3–4 years, and a high number of seeds obtained from a single fruit makes hybridization a very desirable technique [ 9 , 10 , 11 ]. Pomegranate has unique floral biology; flower types are sometimes grouped into two (functional male, hermaphrodite) [ 12 ] and sometimes three (functional male, intermediate, hermaphrodite) categories [ 13 , 14 ]; which significantly vary from genotype to genotype, environmental and agronomic conditions [ 11 , 12 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Pomegranate has a medium juvenile period 3–4 years [ 11 ] and the high number of seeds obtained from a single fruit makes hybridization a very desirable technique [ 9 , 10 , 11 ]. Hybridization is a laborious and time-consuming process.…”

Section: Introductionmentioning

confidence: 99%

Floral Characterization of Pomegranate Genotypes to Improve Hybridization Efficiency

Ikram

Shafqat

Rehman

et al. 2022

Plants

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Pomegranate (Punica granatum) has staminate (male), androgynous (hermaphrodite), and intermediate flower types. Floral characterization is difficult for breeding efficiency across many pomegranate genotypes in Pakistan, which is essential for pomegranate cultivar enhancements. The present research focused on the floral characterization and breeding efficiency of fifteen pomegranate genotypes. Flower sex ratio, floral morphological parameters, i.e., flower length, ovary width, flower notch, flower tip and stigma with style length, and fruit set percentage were examined during the experiment. In terms of sex ratio, male flowers were found to be higher among all genotypes. Due to clear differences in flower length, width, and heterostyly facilitating visual identification of the hermaphrodite flowers, genotype Ternab-2, Kandhari White, and Kandhari Red had higher fruit set (≥70%) among all cross combinations attempted. Genotype Sava had higher flower length and heterostyly of hermaphrodite flower type, but ovary width was not very distinct, leading to average crossing success (85–34%). In conclusion, single or combination of morphological characters can be used for accurate identification of hermaphrodite flowers, which can improve hybrid efficiency and fruit set after artificial cross-pollination.

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Pomegranate (Punica Granatum L.) Breeding

Cited by 16 publications

References 88 publications

Yarrowia lipolytica as a Platform for Punicic Acid Production

Yarrowia lipolytica as a Platform for Punicic Acid Production

Optimization of Development of Pomegranate (Punica granatum L.) Varieties from Microclonal Propagation

Floral Characterization of Pomegranate Genotypes to Improve Hybridization Efficiency

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