Pomegranate extracts on larval inhibition of Haemonchus spp and Cooperia spp obtained from cattle

Abstract

Consumers increasingly demand natural food and absent of toxic waste and medicated. The aim of this study was to evaluate the aqueous extract of dried and in natura stem from pomegranate on the larval inhibition of Haemonchus spp. and Cooperia spp. obtained from cattle. Dried and in natura aqueous extract from pomegranate stem (AEPS), negative control (autoclaved distilled water) and positive control (levamisole hydrochloride 0.05 mg / mL) were evaluated. Four mL of each treatment were used, and the results were evaluated in triplicate by egg counts per gram of feces (EPG), larval culture and counting of the larvae. The mean of the larvae that developed per gram of feces was submitted to logarithimic transformation log. (X + 10). The EPG had an average value of 3.620 eggs, identified in larval culture from Haemonchus spp. and Cooperia spp. Dried AEPS presented, by the reduction of larvae, effectiveness of 92.29% for Haemonchus spp. and 96.97% for Cooperia spp. In natura, this extract showed 80% of efficacy against Cooperia larvae, being considered moderately effective; however, for Haemonchus spp. this extract was not effective. These results are specially important for organic and agroecological systems of production, that not allow the use of chemical parasiticides. In conclusion, the use of dried AEPS showed potential anthelmintic and may represent important natural therapeutic option.

In Vitro Anthelminthic Efficacy of Aqueous Pomegranate (Punica granatum L.) Extracts against Gastrointestinal Nematodes of Sheep

“…The exhibited ovicidal effectiveness is comparable with the TBZ drug. These findings are in line with the in vitro and in vivo studies done by Hassan et al (2020) [ 28 ] and Anjos et al (2016) [ 48 ] that confirmed the efficacy of the P. granatum extract against Haemonchus contortus in goats and Haemonchus spp. and Cooperia spp.…”

Clinical effectiveness of autohemotherapy as an adjuvant in the control of gastrointestinal nematodes in naturally infected sheep

“…Parasitism caused by GIN species is primarily due to the family Trichostrongylidae (3,4) , and results in losses to the national economy (5) . There are several proposals for alternatives to control GIN, such as the selection of resistant breeds (6) , phytotherapy and biological therapy (7,8,9) , nematophagous fungi (10,11) , vaccines against nematodes (12) , and tannin-containing fodder (13) . Isotherapy was initially used in the 1930s by François Lamson, a French homeopath physician, for the treatment of intestinal parasitoses.…”

Anthelmintic and antioxidant potential of banana bracts (Musa paradisiaca) extract in ruminants

“…Medicinal plants have shown promising results in the control of gastrointestinal nematodes of ruminants (ANJOS et al, 2016;MEENAKSHISUNDARAM;HARIKRISHNAN;ANNA, 2016;MUKHERJEE et al, 2016;RAZA, 2015, ROMERO-BENAVIDES et al, 2017SPIEGLER;LIEBAU;HENSEL, 2017). The antiparasitic properties of some tanniniferous plants have been recorded by different research groups (GONÇALVES et al, 2016;U et 7 U et 5 U et al, 2018), specifically including Musa spp.…”