Polysaccharide fractions of Caesalpinia ferrea pods: Potential anti-inflammatory usage

Pereira, Lívia de Paulo; Silva, Racquel Oliveira da; Bringel, Pedro Henrique de Souza Ferreira; Silva, Kaira Emanuella Sales da; Assreuy, Ana Maria Sampaio; Pereira, Maria Lidiane de Sousa

doi:10.1016/j.jep.2011.12.012

Cited by 72 publications

(61 citation statements)

References 22 publications

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“…belongs to the legume family and is an economically important species with multiple applications in construction, which utilizes its wood, and in folk medicine, where fractions of stem and fruit extracts are used to treat bronchopulmonary and gastrointestinal disorders and diabetes (Lucinda et al, 2010;Vasconcelos et al, 2011). Nakamura et al (2002) and Pereira et al (2012) reported the antitumor and anti-inflammatory properties of its fruit, which is an excellent source of energy with a large carbohydrate content that is used to feed ruminants. Nozaki et al (2007) demonstrated that C. ferrea inhibited human topoisomerase II and induced apoptosis in HL60 human leukemia cells, suggesting that this species has potential as an anti-cancer agent.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

et al. 2014

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ABSTRACT. Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpinia ferrea are rare, it is important to develop and/or adapt a DNA extraction protocol that produces quality DNA samples to enable the design of strategies for the conservation of this threatened species. This study aimed to compare five methods for DNA extraction and to determine the most efficient protocol for C. ferrea. Sufficient genomic DNA was obtained from the leaves of C. ferrea using all the tested protocols to perform techniques DNA extraction from Caesalpinia ferrea involving molecular markers. Two protocols based on the detergent cetyl trimethyl ammonium bromide, as well as a commercial kit, yielded high concentrations of pure DNA. However, when polymerase chain reaction amplifications were performed, DNA was only successfully amplified from extractions performed with the commercial kit, which produced sufficient genomic DNA of good quality from the leaves of C. ferrea to perform techniques involving molecular markers.

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Section: Introductionmentioning

confidence: 99%

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

et al. 2014

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“…Anti-infl ammatory, analgesic and antimicrobial properties have been demonstrated, causing interest in continuing with analysis of its pharmacological and therapeutic characteristics [9][10][11][12] . Studies with L. ferrea have demonstrated promising results, and there is a perspective for its use as a mouth wash for dental biofi lm control, because it has antibacterial activity against the microorganisms present in the oral cavity 10,12 .…”

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confidence: 99%

Herbal mouthwash based on Libidibia ferrea: microbiological control, sensory characteristics, sedimentation, pH and density

Venâncio

Rodrigues

Souza

et al. 2015

Rev. odontol. UNESP

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Introduction Phytotherapy is the study of herbal medicines and their applicability to cure diseases in general, being a therapeutic method which can be used for the prevention and treatment of mouth diseases. Among the herbal studied, the Libidibia ferrea, known as jucá or ironwood, is widely used in folk medicine by presenting anti-inflammatory, analgesic, antimicrobial and antipyretic therapeutic properties. Objective To evaluate in vitro pharmacological stability of the Libidibia ferrea extract’s mouthwash (INPA - 228 022). Material and method It was held the mouthwash microbiological control by determining the total number of microorganisms and Salmonella sp, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus; stability characteristics (color, odor, brightness and consistency), sedimentation test (centrifuge), the pH measurement (pH meter) and density evaluation (pycnometer) were analyzed. Result The mouthwash showed to be absent from microorganisms and no changes were observed in the organoleptics and sedimentation characteristics. The average pH values were 6.21, 6.15 and 5.85 at 0, 30 and 60 days, respectively, and 1.029, 1.033 and 1.035 g/ mL density values, respectively, without interfering with the final characteristic of the formulation. Conclusion The mouthwash presented pharmacological stability and quality conditions.

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“…Previous studies have shown biological activities of the extracts of fruit and stem L. ferrea, such as antiinflammatory (Carvalho et al 1996;Pereira et al 2012), and antimicrobial, whereas fruit extract can inhibit in vitro growth of oral pathogens (Sampaio et al 2009), and antioxidant (Silva et al 2011), but this was the first screening with different extracts obtained from L. ferrea evaluated for Leishmania species. However, previous studies showed that the topical use of L. ferrea ointment was significantly efficient to help healing cutaneous wounds in goats (Oliveira et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Antileishmanial activity of extracts from Libidibia ferrea: development of in vitro and in vivo tests

et al. 2017

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Treatment of cutaneous leishmaniasis (CL) is difficult due to the scarce number of drugs able to eliminate completely the intracellular form of the parasite. In the present study, the aim was to evaluate: i) phytochemical properties of extracts from Libidibia ferrea; ii) antileishmanial activity of extracts from L. ferrea against promastigotes and amastigotes of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) guyanensis; iii) the effects of topical treatment using hydrogel containing active extract of L. ferrea on golden hamsters infected with L. (L.) amazonensis. Extracts from leaves, branches and fruits of L. ferrea were obtained with hexane and methanol and were tested by in vitro assays in promastigotes and murine macrophages J774 experimentally infected with amastigotes of Leishmania. Groups of hamsters with CL received topical treatment with a formulation of extract (10%) hydrogels, 50 mg.day ) e baixa citotoxicidade em células de macrófagos J774. Os resultados destacaram que as lesões cutâneas de animais que receberam tratamento com hidrogel + extrato (GelFrMeOH) apresentaram redução significativa (42,78%), menos úlceração e redução da carga parasitária detectada por RT-qPCR, microscopia e análise em cultura e alterações histopatológicas, como menor infiltrado inflamatório após 40 dias de tratamento. A análise química demonstrou que FrMeOH contém altos níveis de compostos fenólicos. Os resultados apontam para uma possível terapia alternativa para CL utilizando fitoterápicos. PALAVRAS-CHAVE: leishmaniose cutânea, formulações tópicas, produtos naturais, tratamento experimental

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Polysaccharide fractions of Caesalpinia ferrea pods: Potential anti-inflammatory usage

Cited by 72 publications

References 22 publications

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

Herbal mouthwash based on Libidibia ferrea: microbiological control, sensory characteristics, sedimentation, pH and density

Antileishmanial activity of extracts from Libidibia ferrea: development of in vitro and in vivo tests

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