Polysaccharide electrospun fibers with sulfated poly(fucose) promote endothelial cell migration and VEGF-mediated angiogenesis

Rujitanaroj, Pim-on; Aid‐Launais, Rachida; Chew, Sing Yian; Visage, Catherine Le

doi:10.1039/c3bm60245a

Cited by 36 publications

(36 citation statements)

References 31 publications

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“…If, during the remodeling of the scaffold, angiogenesis is not initiated, the thickness of the tissue formed will be restricted to the diffusion limit of oxygen and nutrients from the surrounding vasculature (~200 μm thick) [245]. To overcome this, scaffolds such as those designed for cardiac or skeletal muscle that require substantial thickness will benefit from the inclusion of angiogenic growth factors or initiators such as vascular endothelial growth factor (VEGF) to promote endogenous vascularization [248–250]. However, thicker scaffolds will not only need to be chemically and biologically designed to initiate angiogenesis, but must also be physically designed to allow for vessel infiltration.…”

Section: Design Challenges and Future Directionsmentioning

confidence: 99%

Fibrous scaffolds for building hearts and heart parts

Capulli

MacQueen

Sheehy

et al. 2016

Advanced Drug Delivery Reviews

110

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Extracellular matrix (ECM) structure and biochemistry provide cell-instructive cues that promote and regulate tissue growth, function, and repair. From a structural perspective, the ECM is a scaffold that guides the self-assembly of cells into distinct functional tissues. The ECM promotes the interaction between individual cells and between different cell types, and increases the strength and resilience of the tissue in mechanically dynamic environments. From a biochemical perspective, factors regulating cell-ECM adhesion have been described and diverse aspects of cell-ECM interactions in health and disease continue to be clarified. Natural ECMs therefore provide excellent design rules for tissue engineering scaffolds. The design of regenerative three-dimensional (3D) engineered scaffolds is informed by the target ECM structure, chemistry, and mechanics, to encourage cell infiltration and tissue genesis. This can be achieved using nanofibrous scaffolds composed of polymers that simultaneously recapitulate 3D ECM architecture, high-fidelity nanoscale topography, and bio-activity. Their high porosity, structural anisotropy, and bio-activity present unique advantages for engineering 3D anisotropic tissues. Here, we use the heart as a case study and examine the potential of ECM-inspired nanofibrous scaffolds for cardiac tissue engineering. We asked: Do we know enough to build a heart? To answer this question, we tabulated structural and functional properties of myocardial and valvular tissues for use as design criteria, reviewed nanofiber manufacturing platforms and assessed their capabilities to produce scaffolds that meet our design criteria. Our knowledge of the anatomy and physiology of the heart, as well as our ability to create synthetic ECM scaffolds have advanced to the point that valve replacement with nanofibrous scaffolds may be achieved in the short term, while myocardial repair requires further study in vitro and in vivo.

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Section: Design Challenges and Future Directionsmentioning

confidence: 99%

Fibrous scaffolds for building hearts and heart parts

Capulli

MacQueen

Sheehy

et al. 2016

Advanced Drug Delivery Reviews

110

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“…Investigations have also proven its efficacy for nutraceutical and cosmetic applications (Kimura et al, 2013;Atashrazm et al, 2015;Felisilda et al, 2017;Majee et al, 2017a). It is also reported to facilitate neovascularization and angiogenesis through mobilization of endothelial progenitor cells, sequestration and enhancement of the activity of vascular endothelial growth factor (VEGF) (Rujitanaroj et al, 2014). Owing to its characteristic molecular weight, sugar composition and unique charge distribution, ulvan shows great promise with respect to bioactivities and nutritional value (Fernández Diaz et al, 2017).…”

Section: Therapeutic Benefitsmentioning

confidence: 99%

“…Acquired knowledge will also enable in improving product quality and characteristics and also design of novel strains capable of yielding new molecules with modified composition and chain length (Ates, 2015). Alternatively, a list of structural characteristics essential to their optimal performances can be prepared for each of the polymer, against which matching can be done, when isolated from a new source or same source but under different conditions (Rouzet et al, 2011;Rujitanaroj et al, 2014;Salek and Gutierrez, 2016).…”

Section: Future Directionmentioning

confidence: 99%

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Absorption, distribution, metabolism and elimination (ADME) and toxicity profile of marine sulfated polysaccharides used in bionanotechnology

Majee¹,

Avlani²,

Ghosh³

et al. 2018

Afr. J. Pharm. Pharmacol.

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Sulfated polysaccharides extracted from marine algae and bacteria constitute an important class of biomacromolecules as they are characterized by biocompatibility, biodegradability and low immunogenicity. Recent advances in bionanotechnology are attributed to identification of marine sulfated polysaccharides of unique composition and functional properties. Promising results obtained so far justify the need for additional research in the study of absorption, distribution, metabolism and elimination (ADME) of these novel biopolymer-based nanomaterials in human body after administration by oral or parenteral route for therapeutic or diagnostic purpose. In vitro enzymatic degradation pathways should be investigated in order to yield commercially valuable oligomers. The goal of the present review is to enlighten on the ADME, cytotoxicity and in vitro enzymatic degradation of three marine sulfated polysaccharides, fucoidan, ulvan and mauran, obtained from brown seaweeds or macroalgae in the class of Phaeophyceae, members of Ulvales (green algae) and halophilic bacteria, respectively. They are presently being exploited in fabrication of nanoplatforms with novel applications in the field of controlled drug delivery, tissue regeneration scaffolds, cancer therapy, and bioimaging. However, significant research still needs to be carried out to characterize ADME of mauran and to improve production of the biopolymers on a large scale in order to find out clinically relevant solutions to establish these sulfated polysachharide-based nanotools as novel bionanotechnology strategies in future.

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“…Native ECM structure can be mimicked through many ways, such as drawing [7], template synthesis [8], temperature-induced phase separation [9], molecular self-assembly [10], and electrospinning [11][12][13][14][15]. Among these strategies, electrospinning technology is regarded as an efficient preparation method because it can produce nanofibrous scaffolds with interconnected pores, high porosity, and large surface area to mimic native ECM [16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) Nanofibrous Scaffolds for Tissue Engineering

et al. 2016

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Biomimetic scaffolds have been investigated in vascular tissue engineering for many years. Excellent biodegradable materials are desired as temporary scaffolds to support cell growth and disappear gradually with the progress of guided tissue regeneration. In the present paper, a series of biodegradable copolymers were synthesized and used to prepared micro/nanofibrous scaffolds for vascular tissue engineering. Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) [P(LA-co-GA-co-MMD)] copolymers with different L-lactide (LA), glycolide (GA), and 3(S)-methyl-2,5-morpholinedione (MMD) contents were synthesized using stannous octoate as a catalyst. Moreover, the P(LA-co-GA-co-MMD) nanofibrous scaffolds were prepared by electrospinning technology. The morphology of scaffolds was analyzed by scanning electron microscopy (SEM), and the results showed that the fibers are smooth, regular, and randomly oriented with diameters of 700˘100 nm. The weight loss of scaffolds increased significantly with the increasing content of MMD, indicating good biodegradable property of the scaffolds. In addition, the cytocompatibility of electrospun nanofibrous scaffolds was tested by human umbilical vein endothelial cells. It is demonstrated that the cells could attach and proliferate well on P(LA-co-GA-co-MMD) scaffolds and, consequently, form a cell monolayer fully covering on the scaffold surface. Furthermore, the P(LA-co-GA-co-MMD) scaffolds benefit to excellent cell infiltration after subcutaneous implantation. These results indicated that the P(LA-co-GA-co-MMD) nanofibrous scaffolds could be potential candidates for vascular tissue engineering.

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Polysaccharide electrospun fibers with sulfated poly(fucose) promote endothelial cell migration and VEGF-mediated angiogenesis

Abstract: This study demonstrates the potential of fucoidan-incorporated pullulan–dextran fibers as tunable reservoirs for VEGF delivery to promote angiogenesis.

Cited by 36 publications

References 31 publications

Fibrous scaffolds for building hearts and heart parts

Fibrous scaffolds for building hearts and heart parts

Absorption, distribution, metabolism and elimination (ADME) and toxicity profile of marine sulfated polysaccharides used in bionanotechnology

Electrospun Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) Nanofibrous Scaffolds for Tissue Engineering

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