volume 55, issue 5, P819-826 2009
DOI: 10.1111/j.1755-3768.1977.tb08281.x
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HENRIK DAA SCHRøDER, STEFFEN SPERLING

Abstract: Electron microscopy revealed the presence of a 600-1500 A thick layer of polysaccharide on the surface of human corneal endothelial cells. The surface layer was visualized by combined fixation and staining in a mixture of ruthenium red and osmium tetroxide. The coating material was stable for at least 39 h post mortem and was retained on disintegrating cells.