Polyploidy, B chromosomes, and heterochromatin characterization of Mimosa caesalpiniifolia Benth. (Fabaceae-Mimosoideae)

Sousa, Saulo Marçal de; Reis, Aryane Campos; Viccini, Lyderson Facio

doi:10.1007/s11295-012-0567-7

Cited by 12 publications

(5 citation statements)

References 42 publications

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“…Farinosae Barneby had larger chromosomes than other species, and these differences could be associated with variations in the environment and geographic distribution of the studied taxa. Marc ¸al de Sousa et al (2013) arrived to similar conclusions regarding for the karyotype parameters by studying M. caesalpiniifoli Benth., which exhibits B chromosomes.…”

Section: Introductionsupporting

confidence: 58%

Chromosome studies in southern species of Mimosa (Fabaceae, Mimosoideae) and their taxonomic and evolutionary inferences

et al. 2013

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In this work, chromosome numbers and karyotype parameters of 36 taxa of the genus Mimosa were studied, especially from the southern South America center of diversification. Results support that x = 13 is the basic chromosome number in the genus. Polyploidy is very frequent, ca. 56 % of the total of the studied species here are polyploid, confirming that polyploids are more frequent at higher latitudes. The most common ploidy levels found are 2x and 4x, but some species studied exhibit 6x and 8x. In different groups, several ploidy levels were found. Parameters of chromosome size show statistically significant differences between close species, and asymmetry index A 2 exhibited low variation between them. It is possible to infer variations of chromosome size between diploids and tetraploids and between basal and derived taxa. The present studies confirm or reveal polyploidy in several groups of South America which are highly diversified in the southernmost area of distribution of the genus, such as sect. Batocaulon ser. Stipellares and sect. Calothamnos. Our data are discussed in a taxonomic context, making inferences about the origin of some polyploid taxa. Polyploidy could be an important phenomenon that increases the morphologic diversity and specific richness in southern South America. On basis of our data, it is possible to hypothesize hybridization between same-ploidy level or different ploidy level taxa. As already shown in the literature, our results confirm the importance of the polyploidy in the speciation of the genus.

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Section: Introductionsupporting

confidence: 58%

Chromosome studies in southern species of Mimosa (Fabaceae, Mimosoideae) and their taxonomic and evolutionary inferences

et al. 2013

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“…(syn. Mimosa caesalpiniaefolia ) is a native plant to northeastern Brazil, popularly known as “unha-de-gato”, “sabiá”, “angiquinho-sabiá” and “sansão do campo” [19,20]. Due to their therapeutic effects, the stem bark and flowers have been used in traditional medicine for the treatment of bronchitis, skin infections and injuries [21] and for inflammation and hypertension [22], respectively.…”

Section: Introductionmentioning

confidence: 99%

Assessing Chemical Constituents of Mimosa caesalpiniifolia Stem Bark: Possible Bioactive Components Accountable for the Cytotoxic Effect of M. caesalpiniifolia on Human Tumour Cell Lines

et al. 2015

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Mimosa caesalpiniifolia is a native plant of the Brazilian northeast, and few studies have investigated its chemical composition and biological significance. This work describes the identification of the first chemical constituents in the ethanolic extract and fractions of M. caesalpiniifolia stem bark based on NMR, GC-qMS and HRMS analyses, as well as an assessment of their cytotoxic activity. GC-qMS analysis showed fatty acid derivatives, triterpenes and steroid substances and confirmed the identity of the chemical compounds isolated from the hexane fraction. Metabolite biodiversity in M. caesalpiniifolia stem bark revealed the differentiated accumulation of pentacyclic triterpenic acids, with a high content of betulinic acid and minor amounts of 3-oxo and 3β-acetoxy derivatives. OPEN ACCESSMolecules 2015, 20 4205Bioactive analysis based on total phenolic and flavonoid content showed a high amount of these compounds in the ethanolic extract, and ESI-(−)-LTQ-Orbitrap-MS identified caffeoyl hexose at high intensity, as well as the presence of phenolic acids and flavonoids. Furthermore, the evaluation of the ethanolic extract and fractions, including betulinic acid, against colon (HCT-116), ovarian (OVCAR-8) and glioblastoma (SF-295) tumour cell lines showed that the crude extract, hexane and dichloromethane fractions possessed moderate to high inhibitory activity, which may be related to the abundance of betulinic acid. The phytochemical and biological study of M. caesalpiniifolia stem bark thus revealed a new alternative source of antitumour compounds, possibly made effective by the presence of betulinic acid and by chemical co-synergism with other compounds.

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“…However, the karyotype characterization on more population can relieve the interspecific variation in cytogenetic markers. In the Leguminosae family, various patterns of AT-rich and GC-poor heterochromatin were also observed for the Mimosa [ 25 ] and Erythrina [ 26 ] genera. These variations reinforce the need to characterize heterochromatin in other species so as to understand the distribution pattern and evolution of this class of DNA, which is variously colored within the Caesalpinia group.…”

Section: Discussionmentioning

confidence: 99%

Karyotype diversity and 2C DNA content in species of the Caesalpinia group

et al. 2018

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BackgroundThe Leguminosae family is the third-largest family of angiosperms, and Caesalpinioideae is its second-largest subfamily. A great number of species (approximately 205) are found in the Caesalpinia group within this subfamily; together with these species’ phenotypic plasticity and the similarities in their morphological descriptors, make this a complex group for taxonomic and phylogenetic studies. The objective of the present work was to evaluate the karyotypic diversity and the 2C DNA content variation in 10 species of the Caesalpinia group, representing six genera: Paubrasilia, Caesalpinia, Cenostigma, Poincianella, Erythrostemon and Libidibia. The GC-rich heterochromatin and 45S rDNA sites (which are used as chromosome markers) were located to evaluate the karyotype diversity in the clade. The variation in the 2C DNA content was determined through flow cytometry.ResultsThe fluorochrome banding indicated that the chromomycin A3+/4′,6-diamidino-2-phenylindole− blocks were exclusively in the terminal regions of the chromosomes, coinciding with 45S rDNA sites in all analyzed species. Physical mapping of the species (through fluorescence in situ hybridization) revealed variation in the size of the hybridization signals and in the number and distribution of the 45S rDNA sites. All hybridization sites were in the terminal regions of the chromosomes. In addition, all species had a hybridization site in the fourth chromosome pair. The 2C DNA content ranged from 1.54 pg in Erythrostemon calycina to 2.82 pg in the Paubrasilia echinata large-leaf variant. The Pa. echinata small-leaf variant was isolated from the other leaf variants through Scoot-Knott clustering.ConclusionsThe chromosome diversity and the variation in the 2C DNA content reinforce that the actual taxonomy and clustering of the analyzed taxa requires more genera that were previously proposed. This fact indicates that taxonomy, phylogeny and cytoevolutionary inference related to the complex Caesalpinia group have to be done through integrative evaluation.

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Polyploidy, B chromosomes, and heterochromatin characterization of Mimosa caesalpiniifolia Benth. (Fabaceae-Mimosoideae)

Cited by 12 publications

References 42 publications

Chromosome studies in southern species of Mimosa (Fabaceae, Mimosoideae) and their taxonomic and evolutionary inferences

Chromosome studies in southern species of Mimosa (Fabaceae, Mimosoideae) and their taxonomic and evolutionary inferences

Assessing Chemical Constituents of Mimosa caesalpiniifolia Stem Bark: Possible Bioactive Components Accountable for the Cytotoxic Effect of M. caesalpiniifolia on Human Tumour Cell Lines

Karyotype diversity and 2C DNA content in species of the Caesalpinia group

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